| dc.contributor.author |
Cheng, K |
en |
| dc.contributor.author |
Kisaalita, WS |
en |
| dc.date.accessioned |
2014-06-06T06:49:46Z |
|
| dc.date.available |
2014-06-06T06:49:46Z |
|
| dc.date.issued |
2010 |
en |
| dc.identifier.issn |
87567938 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1002/btpr.391 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/4776 |
|
| dc.subject |
Adhesion |
en |
| dc.subject |
Micro-/nano-fabrication |
en |
| dc.subject |
Scaffold |
en |
| dc.subject |
Stem cell |
en |
| dc.subject |
Tissue engineering |
en |
| dc.subject.other |
Architectural features |
en |
| dc.subject.other |
Cell infiltration |
en |
| dc.subject.other |
Cellular activities |
en |
| dc.subject.other |
Cellular adhesion |
en |
| dc.subject.other |
Extracellular matrices |
en |
| dc.subject.other |
Hybrid polymers |
en |
| dc.subject.other |
matrix |
en |
| dc.subject.other |
Micro-/nano-fabrication |
en |
| dc.subject.other |
Micro-scales |
en |
| dc.subject.other |
Micropores |
en |
| dc.subject.other |
Nano scale |
en |
| dc.subject.other |
Nanofabrication |
en |
| dc.subject.other |
Nanoscale structure |
en |
| dc.subject.other |
Particulate leaching |
en |
| dc.subject.other |
Polymer scaffolds |
en |
| dc.subject.other |
Proliferation rate |
en |
| dc.subject.other |
Stem cell |
en |
| dc.subject.other |
Stem cell tissue engineering |
en |
| dc.subject.other |
Adhesion |
en |
| dc.subject.other |
Cell culture |
en |
| dc.subject.other |
Fabrication |
en |
| dc.subject.other |
Leaching |
en |
| dc.subject.other |
Nanofibers |
en |
| dc.subject.other |
Nanostructured materials |
en |
| dc.subject.other |
Nanotechnology |
en |
| dc.subject.other |
Phase separation |
en |
| dc.subject.other |
Stem cells |
en |
| dc.subject.other |
Three dimensional |
en |
| dc.subject.other |
Tissue |
en |
| dc.subject.other |
Tissue engineering |
en |
| dc.subject.other |
Scaffolds |
en |
| dc.subject.other |
nanomaterial |
en |
| dc.subject.other |
tissue scaffold |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
cell adhesion |
en |
| dc.subject.other |
cell culture |
en |
| dc.subject.other |
cell differentiation |
en |
| dc.subject.other |
chemistry |
en |
| dc.subject.other |
comparative study |
en |
| dc.subject.other |
cytology |
en |
| dc.subject.other |
fibroblast |
en |
| dc.subject.other |
fluorescence microscopy |
en |
| dc.subject.other |
human |
en |
| dc.subject.other |
materials testing |
en |
| dc.subject.other |
methodology |
en |
| dc.subject.other |
nerve cell |
en |
| dc.subject.other |
porosity |
en |
| dc.subject.other |
scanning electron microscopy |
en |
| dc.subject.other |
shear strength |
en |
| dc.subject.other |
stem cell |
en |
| dc.subject.other |
tissue engineering |
en |
| dc.subject.other |
ultrastructure |
en |
| dc.subject.other |
Cell Adhesion |
en |
| dc.subject.other |
Cell Differentiation |
en |
| dc.subject.other |
Cells, Cultured |
en |
| dc.subject.other |
Fibroblasts |
en |
| dc.subject.other |
Humans |
en |
| dc.subject.other |
Materials Testing |
en |
| dc.subject.other |
Microscopy, Electron, Scanning |
en |
| dc.subject.other |
Microscopy, Fluorescence |
en |
| dc.subject.other |
Nanostructures |
en |
| dc.subject.other |
Neurons |
en |
| dc.subject.other |
Porosity |
en |
| dc.subject.other |
Shear Strength |
en |
| dc.subject.other |
Stem Cells |
en |
| dc.subject.other |
Tissue Engineering |
en |
| dc.subject.other |
Tissue Scaffolds |
en |
| dc.title |
Exploring cellular adhesion and differentiation in a micro-/nano-hybrid polymer scaffold |
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| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1002/btpr.391 |
en |
| heal.publicationDate |
2010 |
en |
| heal.abstract |
Polymer scaffolds play an important role in three dimensional (3-D) cell culture and tissue engineering. To best mimic the archiecture of natural extracellular matrix (ECM), a nano-fibrous and micro-porous combined (NFMP) scaffold was fabricated by combining phase separation and particulate leaching techniques. The NFMP scaffold possesses architectural features at two levels, including the micro-scale pores and nano-scale fibers. To evaluate the advantages of micro/nano combination, control scaffolds with only micro-pores or nano-fibers were fabricated. Cell grown in NFMP and control scaffolds were characterized with respect to morphology, proliferation rate, diffentiation and adhesion. The NFMP scaffold combined the advantages of micro- and nano-scale structures. The NFMP scaffold nano-fibers promoted neural differentiation and induced ""3-D matrix adhesion"", while the NFMP scaffold micro-pores facilitated cell infiltration. This study represents a systematic comparison of cellular activities on micro-only, nano-only and micro/nano combined scaffolds, and demonstrates the unique advantages of the later. © 2010 American Institute of Chemical Engineers. |
en |
| heal.journalName |
Biotechnology Progress |
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| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
26 |
en |
| dc.identifier.doi |
10.1002/btpr.391 |
en |
| dc.identifier.spage |
838 |
en |
| dc.identifier.epage |
846 |
en |