Effect of biochemical stimulants on biomass productivity and metabolite content of the microalga, Chlorella sorokiniana

dc.contributor.author	Hunt, RW	en
dc.contributor.author	Chinnasamy, S	en
dc.contributor.author	Bhatnagar, A	en
dc.contributor.author	Das, KC	en
dc.date.accessioned	2014-06-06T06:49:45Z
dc.date.available	2014-06-06T06:49:45Z
dc.date.issued	2010	en
dc.identifier.issn	02732289	en
dc.identifier.uri	http://dx.doi.org/10.1007/s12010-010-9012-2	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4763
dc.subject	Auxins	en
dc.subject	Bioenergy	en
dc.subject	Biofuels	en
dc.subject	Biomass	en
dc.subject	Biostimulants	en
dc.subject	Microalgae	en
dc.subject	Phytohormones	en
dc.subject.other	Auxins	en
dc.subject.other	Bio-energy	en
dc.subject.other	Biostimulants	en
dc.subject.other	Micro-algae	en
dc.subject.other	Phytohormones	en
dc.subject.other	Algae	en
dc.subject.other	Biofuels	en
dc.subject.other	Biomass	en
dc.subject.other	Chlorination	en
dc.subject.other	Chlorine compounds	en
dc.subject.other	Chlorophyll	en
dc.subject.other	Cost reduction	en
dc.subject.other	Fatty acids	en
dc.subject.other	Metabolism	en
dc.subject.other	Metabolites	en
dc.subject.other	Methanol	en
dc.subject.other	Microorganisms	en
dc.subject.other	Organic acids	en
dc.subject.other	Porphyrins	en
dc.subject.other	Productivity	en
dc.subject.other	Ecology	en
dc.subject.other	1 naphthylacetic acid	en
dc.subject.other	2 phenylacetic acid	en
dc.subject.other	auxin	en
dc.subject.other	biofuel	en
dc.subject.other	chlorophyll	en
dc.subject.other	ferric chloride	en
dc.subject.other	gibberellic acid	en
dc.subject.other	humic acid	en
dc.subject.other	indolebutyric acid	en
dc.subject.other	kelp extract	en
dc.subject.other	methanol	en
dc.subject.other	phytohormone	en
dc.subject.other	plant extract	en
dc.subject.other	polyamine	en
dc.subject.other	putrescine	en
dc.subject.other	spermidine	en
dc.subject.other	thidiazuron	en
dc.subject.other	trace element	en
dc.subject.other	unclassified drug	en
dc.subject.other	zeatin	en
dc.subject.other	article	en
dc.subject.other	biomass production	en
dc.subject.other	Chlorella sorokiniana	en
dc.subject.other	metabolite	en
dc.subject.other	nonhuman	en
dc.subject.other	plant growth	en
dc.subject.other	Biomass	en
dc.subject.other	Biotechnology	en
dc.subject.other	Chlorella	en
dc.subject.other	Industry	en
dc.subject.other	Organic Chemicals	en
dc.subject.other	Time Factors	en
dc.subject.other	Chlorella sorokiniana	en
dc.subject.other	Chlorophyta	en
dc.subject.other	Paa	en
dc.title	Effect of biochemical stimulants on biomass productivity and metabolite content of the microalga, Chlorella sorokiniana	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s12010-010-9012-2	en
heal.publicationDate	2010	en
heal.abstract	The influence of 12 biochemical stimulants, namely 2-phenylacetic acid (PAA; 30 ppm), indole-3 butyric acid (IBA; 10 ppm), 1-naphthaleneacetic acid (NAA; 2.5, 5 and 10 ppm ), gibberellic acid (GA3, 10 ppm), zeatin (ZT; 0.002 ppm), thidiazuron (0.22 ppm), humic acid (20 ppm), kelp extract (250 ppm), methanol (500 ppm), ferric chloride (3.2 ppm ), putrescine (0.09 ppm), spermidine (1.5 ppm) were prescreened for their impact on growth and chlorophyll for the green alga-Chlorella sorokiniana. C. sorokiniana responded best to phytohormones in the auxin family, particularly NAA. Thereafter, two studies were conducted on combinations of phytohormones to compare blends from within the auxin family as well as against other families. These treatments were NAA5 ppm+PAA30 ppm, NAA2.5 ppm+PAA 15 ppm, NAA5 ppm+IBA10 ppm, NAA 5 ppm+GA310 ppm, NAA5 ppm+ZT1 ppm, and NAA5 ppm+GA310 ppm+ZT1 ppm. Combinations of NAA with other auxins did not have synergistic or antagonistic effects on the growth. However, combinations of compounds from different phytohormone families, such as NAA5 ppm+GA310 ppm+ZT1 ppm, dramatically increased the biomass productivity by 170% over the control followed by the treatments: NAA 5 ppm+GA310 ppm (138%), NAA 5 ppm+ZT1 ppm (136%), and NAA 5 ppm ( 133%). The effect of biochemical stimulants were also measured on metabolites such as chlorophyll, protein, and lipids in C. sorokiniana. Renewed interest in microalgae for biotechnology and biofuel applications may warrant the use of biochemical stimulants for cost reduction in large-scale cultivation through increased biomass productivity. © 2010 Springer Science+Business Media, LLC.	en
heal.journalName	Applied Biochemistry and Biotechnology	en
dc.identifier.issue	8	en
dc.identifier.volume	162	en
dc.identifier.doi	10.1007/s12010-010-9012-2	en
dc.identifier.spage	2400	en
dc.identifier.epage	2414	en