| dc.contributor.author |
Ray, LE |
en |
| dc.contributor.author |
Scherzer, J |
en |
| dc.contributor.author |
Graves, WM |
en |
| dc.contributor.author |
Heusner, GL |
en |
| dc.date.accessioned |
2014-06-06T06:49:41Z |
|
| dc.date.available |
2014-06-06T06:49:41Z |
|
| dc.date.issued |
2010 |
en |
| dc.identifier.issn |
0043535X |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/4726 |
|
| dc.relation.uri |
http://www.scopus.com/inward/record.url?eid=2-s2.0-77953863880&partnerID=40&md5=3bba5c9cbbb50953ced4d975e8987af4 |
en |
| dc.subject |
Blastocoelic micromanipulation |
en |
| dc.subject |
Cryopreservation |
en |
| dc.subject |
Porcine embryo |
en |
| dc.subject |
Vitrification |
en |
| dc.subject.other |
Sus |
en |
| dc.title |
A novel approach to cryopreservation of porcine embryos by vitrification after blastocoelic micromanipulation |
en |
| heal.type |
journalArticle |
en |
| heal.publicationDate |
2010 |
en |
| heal.abstract |
In this study, the effects of the reduction of blastocoelic fluid prior to vitrification were examined on porcine embryos (n=44; 160-540 μm in size; graded excellent (1) according to IETS guidelines). The embryos were either assigned to a control group (n=24) or to the experimental group (n=20) which differed in that blastocoelic fluid was aspirated before microinfusion of vitrification solution 1 (VS1; 1.4 M glycerol in PBS). During the vitrification procedure, the embryos were exposed to 2 cryopreservation solutions, VS1 and VS2 (1.4 M glycerol plus 3.6 M ethylene glycol in PBS), for 5 minutes each, and then to a third cryopreservative, VS3 (3.4 M glycerol and 6.6 M ethylene glycol in PBS), for 1 minute. The straws with embryos were placed in a cooled plastic goblet surrounded by liquid nitrogen vapors for 1 minute and then immersed into the liquid nitrogen for rapid freezing through the phase transition point. The embryos were thawed at 30 °C for 15 s in a water bath and cultured for a period of 24 hrs in NCSU media. Morphological evaluation of the embryos using a stereomicroscope revealed, that initial re-expansion was complete in 16 of the treated and 7 of the control embryos. This demonstrated a significant difference between treatment and control groups at thaw in the recovery of apparently functional embryos (p < 0.001). All embryos from both treatments were degenerated after 24 hrs of culture. |
en |
| heal.journalName |
Wiener Tierarztliche Monatsschrift |
en |
| dc.identifier.issue |
5-6 |
en |
| dc.identifier.volume |
97 |
en |
| dc.identifier.spage |
135 |
en |
| dc.identifier.epage |
140 |
en |