The effect of substrate, ADP and uncoupler on the respiration of tomato pollen during incubation in vitro at moderately high temperature

dc.contributor.author	Karapanos, IC	en
dc.contributor.author	Akoumianakis, KA	en
dc.contributor.author	Olympios, CM	en
dc.contributor.author	Passam, HC	en
dc.date.accessioned	2014-06-06T06:49:36Z
dc.date.available	2014-06-06T06:49:36Z
dc.date.issued	2009	en
dc.identifier.issn	09340882	en
dc.identifier.uri	http://dx.doi.org/10.1007/s00497-009-0098-z	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4680
dc.subject	CCCP	en
dc.subject	High-temperature stress	en
dc.subject	In vitro incubation	en
dc.subject	NADH	en
dc.subject	Succinate	en
dc.subject.other	adenosine diphosphate	en
dc.subject.other	carbon dioxide	en
dc.subject.other	carbonyl cyanide chlorophenylhydrazone	en
dc.subject.other	nicotinamide adenine dinucleotide	en
dc.subject.other	succinic acid	en
dc.subject.other	article	en
dc.subject.other	cell respiration	en
dc.subject.other	heat	en
dc.subject.other	metabolism	en
dc.subject.other	physiology	en
dc.subject.other	pollen	en
dc.subject.other	tomato	en
dc.subject.other	Adenosine Diphosphate	en
dc.subject.other	Carbon Dioxide	en
dc.subject.other	Carbonyl Cyanide m-Chlorophenyl Hydrazone	en
dc.subject.other	Cell Respiration	en
dc.subject.other	Hot Temperature	en
dc.subject.other	Lycopersicon esculentum	en
dc.subject.other	NAD	en
dc.subject.other	Pollen	en
dc.subject.other	Succinic Acid	en
dc.subject.other	Lycopersicon esculentum	en
dc.title	The effect of substrate, ADP and uncoupler on the respiration of tomato pollen during incubation in vitro at moderately high temperature	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00497-009-0098-z	en
heal.publicationDate	2009	en
heal.abstract	Pollen of tomato cv. Supermarmande was collected from greenhouse-grown plants at various intervals throughout the year and arbitrarily classified as of high, medium or low respiratory activity on the basis of CO2 production during 8 h incubation in vitro at 30°C, a temperature that is considered to be moderately high for tomato fruit set. After an initial burst of respiration during the first stage of hydration at 30°C (>1 h), the respiration rate of pollen of all three categories declined, the decrease being greater in the lots with a low or medium respiratory activity than in the high category. During hydration (10 min after the start of incubation), the addition of succinate or reduced β-nicotinamide adenine dinucleotide (NADH) to the substrate increased the respiratory rate of slowly-respiring pollen more than that of fast-respiring pollen, but carbonyl cyanide 3-chlorophenylhydrazone (CCCP) and adenosine 5′-diphosphate (ADP) had less effect. After 1-4 h incubation, the respiration rate of the slow- or medium-respiring pollen lots had decreased, but was stimulated by succinate or NADH, and to a lesser degree by ADP. By 7 h, the respiration rate of all pollen lots had declined and was stimulated less by substrate, ADP or CCCP. The oxidation of NADH by tomato pollen contrasts with the failure of other pollen species to utilize this substrate; moreover, a synergistic effect of NADH and succinate was consistently observed. We conclude that the decline in respiration during incubation for up to 4 h at 30°C may reflect a lack of respiratory substrate. After 7 h, however, the decreased response to substrate indicates a loss of mitochondrial integrity or an accumulation of metabolic inhibitors. It is concluded that at 30°C (a moderately high temperature for tomato pollen), the initially high rate of respiration leads to exhaustion of the endogenous respiratory substrates (particularly in pollen with low to medium respiratory activity), but subsequently to ageing and a loss of mitochondrial activity. © Springer-Verlag 2009.	en
heal.journalName	Sexual Plant Reproduction	en
dc.identifier.issue	3	en
dc.identifier.volume	22	en
dc.identifier.doi	10.1007/s00497-009-0098-z	en
dc.identifier.spage	133	en
dc.identifier.epage	140	en