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Sheep and goats differences in CLA and fatty acids milk fat content in relation with mRNA stearoyl-CoA desaturase and lipogenic genes expression in their mammary gland

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dc.contributor.author Tsiplakou, E en
dc.contributor.author Flemetakis, E en
dc.contributor.author Kalloniati, C en
dc.contributor.author Papadomichelakis, G en
dc.contributor.author Katinakis, P en
dc.contributor.author Zervas, G en
dc.date.accessioned 2014-06-06T06:49:31Z
dc.date.available 2014-06-06T06:49:31Z
dc.date.issued 2009 en
dc.identifier.issn 00220299 en
dc.identifier.uri http://dx.doi.org/10.1017/S0022029909990100 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/4649
dc.subject δ-9-desaturase en
dc.subject Animal species en
dc.subject Conjugated linoleic acid en
dc.subject Lipogenic genes en
dc.subject.other acyl coenzyme A desaturase en
dc.subject.other conjugated linoleic acid en
dc.subject.other fatty acid en
dc.subject.other messenger RNA en
dc.subject.other animal en
dc.subject.other animal food en
dc.subject.other article en
dc.subject.other chemistry en
dc.subject.other clinical trial en
dc.subject.other controlled clinical trial en
dc.subject.other controlled study en
dc.subject.other diet en
dc.subject.other female en
dc.subject.other gene expression regulation en
dc.subject.other genetics en
dc.subject.other goat en
dc.subject.other metabolism en
dc.subject.other milk en
dc.subject.other physiology en
dc.subject.other sheep en
dc.subject.other udder en
dc.subject.other Animal Feed en
dc.subject.other Animals en
dc.subject.other Diet en
dc.subject.other Fatty Acids en
dc.subject.other Female en
dc.subject.other Gene Expression Regulation en
dc.subject.other Goats en
dc.subject.other Linoleic Acids, Conjugated en
dc.subject.other Mammary Glands, Animal en
dc.subject.other Milk en
dc.subject.other RNA, Messenger en
dc.subject.other Sheep en
dc.subject.other Stearoyl-CoA Desaturase en
dc.subject.other Animalia en
dc.subject.other Capra hircus en
dc.subject.other Ovis aries en
dc.title Sheep and goats differences in CLA and fatty acids milk fat content in relation with mRNA stearoyl-CoA desaturase and lipogenic genes expression in their mammary gland en
heal.type journalArticle en
heal.identifier.primary 10.1017/S0022029909990100 en
heal.publicationDate 2009 en
heal.abstract An experiment was conducted with 12 lactating dairy ewes and 12 goats with the objective to determine whether, under the same dietary treatments, the differences in their fatty acid (FA) profile with emphasis on cis-9 trans-11 CLA milk fat content, are reflected in the transcript levels of genes involved in FA and cis-9, trans-11 CLA biosynthesis. The animals were fed with two diets (A, B) in different days of milk (DIM) due to the different milk yield, body weight etc, in order to have the same food intake and to avoid dietary effects. Diet A was fed to the animals on a group basis as it is traditionally used in practice, while diet B was chosen to avoid individual feed intake variation which is usually observed in group feeding. The results showed that there are significantly lower mRNA levels of acetyl-CoA carboxylase (ACC) in sheep mammary gland compared with those of goats, independently from the diet fed. The same trend was observed with the mRNA level of FA synthase (FAS), but the results were significant only for diet A. The mRNA level of lipoprotein lipase (LPL) in the mammary gland did not differ between sheep and goats fed with diet A. In addition, the concentration of cis-9 trans-11 CLA content was significantly higher in sheep milk fat compared with those of goats. This is in accordance with the significant higher levels on mRNA of stearoyl-CoA desaturase (SCD) which were observed in their mammary adipocytes of sheep compared with those of goats, independently of the fed diet (A or B). In conclusion, these findings demonstrate that the differences between sheep and goats, concerning cis-9, trans-11 CLA and FA milk fat content, under the same dietary treatments could be explained in part by the differences in mRNA of SCD and lipogenic genes in their mammary gland. © Proprietors of Journal of Dairy Research 2009. en
heal.journalName Journal of Dairy Research en
dc.identifier.issue 4 en
dc.identifier.volume 76 en
dc.identifier.doi 10.1017/S0022029909990100 en
dc.identifier.spage 392 en
dc.identifier.epage 401 en


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