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Juvenile hormone induces the expression of the SnoSP2 gene encoding a methionine-rich hexamerin in Sesamia nonagrioides (Lepidoptera)

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dc.contributor.author Gkouvitsas, T en
dc.contributor.author Kourti, A en
dc.date.accessioned 2014-06-06T06:49:27Z
dc.date.available 2014-06-06T06:49:27Z
dc.date.issued 2009 en
dc.identifier.issn 10964959 en
dc.identifier.uri http://dx.doi.org/10.1016/j.cbpb.2009.02.017 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/4604
dc.subject Diapause en
dc.subject Insect en
dc.subject Juvenile hormone induced protein en
dc.subject Lepidoptera en
dc.subject Methionine-rich storage protein en
dc.subject mRNA developmental expression en
dc.subject Noctuidae en
dc.subject Sesamia nonagrioides en
dc.subject.other hexamerin en
dc.subject.other juvenile hormone en
dc.subject.other juvenile hormone induced protein en
dc.subject.other messenger RNA en
dc.subject.other methionine en
dc.subject.other protein en
dc.subject.other signal peptide en
dc.subject.other unclassified drug en
dc.subject.other amino acid sequence en
dc.subject.other animal tissue en
dc.subject.other article en
dc.subject.other gene en
dc.subject.other gene expression en
dc.subject.other hemolymph en
dc.subject.other insect en
dc.subject.other larva en
dc.subject.other Lepidoptera en
dc.subject.other molecular cloning en
dc.subject.other nonhuman en
dc.subject.other nucleotide sequence en
dc.subject.other phylogeny en
dc.subject.other priority journal en
dc.subject.other real time polymerase chain reaction en
dc.subject.other reverse transcription polymerase chain reaction en
dc.subject.other Sesamia nogrioides en
dc.subject.other SnoSP2 gene en
dc.subject.other Amino Acid Sequence en
dc.subject.other Animals en
dc.subject.other Cloning, Molecular en
dc.subject.other Insect Proteins en
dc.subject.other Juvenile Hormones en
dc.subject.other Larva en
dc.subject.other Lepidoptera en
dc.subject.other Methionine en
dc.subject.other Molecular Sequence Data en
dc.subject.other Phylogeny en
dc.subject.other Pupa en
dc.subject.other RNA, Messenger en
dc.subject.other Hexapoda en
dc.subject.other Lepidoptera en
dc.subject.other Noctuidae en
dc.subject.other Papaipema nebris en
dc.subject.other Sesamia nonagrioides en
dc.subject.other Spodoptera litura en
dc.subject.other Zea mays en
dc.title Juvenile hormone induces the expression of the SnoSP2 gene encoding a methionine-rich hexamerin in Sesamia nonagrioides (Lepidoptera) en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.cbpb.2009.02.017 en
heal.publicationDate 2009 en
heal.abstract A gene encoding a methionine-rich storage protein, SnoSP2, was cloned and its complete cDNA sequence was determined in the corn stalk borer, Sesamia nonagrioides (Lepidoptera: Noctuidae). Potentially, SnoSP2 encoded a 748-amino acid protein, with a calculated molecular weight of 87.9 kDa and an isoelectric point pI = 9.41. Signal peptide of 15 amino acids is present at the N-terminus and the protein contained conserved insect larval storage protein signature sequence patterns. The deduced amino acid sequence of SnoSP2 showed the highest identity to the methionine-rich storage protein from Spodoptera litura (77%) and other methionine-rich storage proteins. SnoSP2 belongs to the subfamily of methionine-rich storage proteins (6.8% methionine, 9.5% aromatic amino acid), according to criteria of amino acid composition and phylogenetic analysis. Expression of SnoSP2 mRNA was determined using semi-quantitative RT-PCR and real-time PCR. When larvae were treated with juvenile-hormone analog, methroprene, SnoSP2 transcripts were induced. In non-diapausing conditions, the SnoSP2 mRNA presents in the beginning of fifth instar, increased dramatically during the sixth instar, peaked in the end of sixth instar, decreased in the early pupae and were very low at the late pupae. In diapausing conditions, SnoSP2 remain abundant through the pre-diapause, persists through deep diapause and disappear in the end of post-diapause phase. © 2009 Elsevier Inc. All rights reserved. en
heal.journalName Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology en
dc.identifier.issue 2 en
dc.identifier.volume 153 en
dc.identifier.doi 10.1016/j.cbpb.2009.02.017 en
dc.identifier.spage 206 en
dc.identifier.epage 215 en


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