Impact of nitrogen and sulfur fertilization on the composition of glucosinolates in relation to sulfur assimilation in different plant organs of broccoli

dc.contributor.author	Omirou, MD	en
dc.contributor.author	Papadopoulou, KK	en
dc.contributor.author	Papastylianou, I	en
dc.contributor.author	Constantinou, M	en
dc.contributor.author	Karpouzas, DG	en
dc.contributor.author	Asimakopoulos, I	en
dc.contributor.author	Ehaliotis, C	en
dc.date.accessioned	2014-06-06T06:49:26Z
dc.date.available	2014-06-06T06:49:26Z
dc.date.issued	2009	en
dc.identifier.issn	00218561	en
dc.identifier.uri	http://dx.doi.org/10.1021/jf901440n	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4594
dc.subject	Broccoli	en
dc.subject	Glucosinolates	en
dc.subject	HPLC	en
dc.subject	Nitrogen fertilization	en
dc.subject	Sulfur	en
dc.subject.other	glucosinolate	en
dc.subject.other	nitrogen	en
dc.subject.other	sulfur	en
dc.subject.other	article	en
dc.subject.other	Brassica	en
dc.subject.other	chemistry	en
dc.subject.other	histology	en
dc.subject.other	metabolism	en
dc.subject.other	plant leaf	en
dc.subject.other	plant root	en
dc.subject.other	Brassica	en
dc.subject.other	Glucosinolates	en
dc.subject.other	Nitrogen	en
dc.subject.other	Plant Leaves	en
dc.subject.other	Plant Roots	en
dc.subject.other	Sulfur	en
dc.subject.other	Brassica oleracea var. capitata	en
dc.subject.other	Brassica oleracea var. italica	en
dc.title	Impact of nitrogen and sulfur fertilization on the composition of glucosinolates in relation to sulfur assimilation in different plant organs of broccoli	en
heal.type	journalArticle	en
heal.identifier.primary	10.1021/jf901440n	en
heal.publicationDate	2009	en
heal.abstract	Broccoli (Brassica oleracea var. italica) is one of the most important winter season vegetables and a rich source of chemoprotective molecules, including glucosinolates (GSL). The aim of this study was to investigate the impact of nitrogen (N) and sulfur (S) fertilization on GSL concentration and composition in different parts of broccoli plants. A greenhouse experiment was performed, with four different treatments of sulfur (10, 30, 70, and 150 kg/ha) and three treatments of nitrogen (50, 250, and 600 kg/ha). GSL concentrations and plant growth responded to the N supply, but this was not observed above the 250 kg N/ha dose. On the contrary, plant growth did not respond to the S supply, whereas GSL concentrations showed a sharp response to the whole range of S applications (from 10 to 150 kg/ha). Glucosinolate composition was altered differentially in the examined plant parts. Aliphatic GSL were more abundant in the florets and leaves, whereas indolyl GSLs were dominant in roots, in which aromatic GSL were also observed. High nitrogen fertilization had a higher impact on indolyl compared to aliphatic GSLs concentration. More importantly, a high concentration of aliphatic GSL, >2.4 μmol/g dry weight (dw), and high S assimilation into aliphatic GSL were consistently observed in the florets compared to other broccoli parts, indicating adaptable processes for nitrogen and sulfur regarding synthesis and transport of aliphatic GSL for these organs. © 2009 American Chemical Society.	en
heal.journalName	Journal of Agricultural and Food Chemistry	en
dc.identifier.issue	20	en
dc.identifier.volume	57	en
dc.identifier.doi	10.1021/jf901440n	en
dc.identifier.spage	9408	en
dc.identifier.epage	9417	en