Immobilization of electroporated cells for fabrication of cellular biosensors: Physiological effects of the shape of calcium alginate matrices and foetal calf serum

dc.contributor.author	Katsanakis, N	en
dc.contributor.author	Katsivelis, A	en
dc.contributor.author	Kintzios, S	en
dc.date.accessioned	2014-06-06T06:49:26Z
dc.date.available	2014-06-06T06:49:26Z
dc.date.issued	2009	en
dc.identifier.issn	14248220	en
dc.identifier.uri	http://dx.doi.org/10.3390/s90100378	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4593
dc.subject	Calcium alginate	en
dc.subject	Cell immobilization	en
dc.subject	Electroporation	en
dc.subject	Foetal calf serum (FCS)	en
dc.subject	Monkey African green kidney (Vero) cells	en
dc.subject.other	Calcium alginate	en
dc.subject.other	Calcium alginate gels	en
dc.subject.other	Culture atmospheres	en
dc.subject.other	Culture conditions	en
dc.subject.other	Electroporation	en
dc.subject.other	Foetal calf serums	en
dc.subject.other	Physiological effects	en
dc.subject.other	Suspension cultures	en
dc.subject.other	Alginate	en
dc.subject.other	Biosensors	en
dc.subject.other	Body fluids	en
dc.subject.other	Calcium	en
dc.subject.other	Carbon dioxide	en
dc.subject.other	Cell proliferation	en
dc.subject.other	Cells	en
dc.subject.other	Cytology	en
dc.subject.other	Gels	en
dc.subject.other	Physiology	en
dc.subject.other	Cell immobilization	en
dc.title	Immobilization of electroporated cells for fabrication of cellular biosensors: Physiological effects of the shape of calcium alginate matrices and foetal calf serum	en
heal.type	journalArticle	en
heal.identifier.primary	10.3390/s90100378	en
heal.publicationDate	2009	en
heal.abstract	In order to investigate the physiological effect of transfected cell immobilization in calcium alginate gels, we immobilized electroporated Vero cells in gels shaped either as spherical beads or as thin membrane layers. In addition, we investigated whether serum addition had a positive effect on cell proliferation and viability in either gel configuration. The gels were stored for four weeks in a medium supplemented or not with 20% (v/v) foetal calf serum. Throughout a culture period of four weeks, cell proliferation and cell viability were assayed by optical microscopy after provision of Trypan Blue. Non-elaborate culture conditions (room temperature, non-CO2 enriched culture atmosphere) were applied throughout the experimental period in order to evaluate cell viability under less than optimal storage conditions. Immobilization of electroporated cells was associated with an initially reduced cell viability, which was gradually increased. Immobilization was associated with maintenance of cell growth for the duration of the experimental period, whereas electroporated cells essentially died after a week in suspension culture. Considerable proliferation of immobilized cells was observed in spherical alginate beads. In both gel configurations, addition of serum was associated with increased cell proliferation. The results of the present study could contribute to an improvement of the storability of biosensors based on electroporated, genetically or membrane-engineered cells.	en
heal.journalName	Sensors	en
dc.identifier.issue	1	en
dc.identifier.volume	9	en
dc.identifier.doi	10.3390/s90100378	en
dc.identifier.spage	378	en
dc.identifier.epage	385	en