| dc.contributor.author |
Tsagkarakou, A |
en |
| dc.contributor.author |
Van Leeuwen, T |
en |
| dc.contributor.author |
Khajehali, J |
en |
| dc.contributor.author |
Ilias, A |
en |
| dc.contributor.author |
Grispou, M |
en |
| dc.contributor.author |
Williamson, MS |
en |
| dc.contributor.author |
Tirry, L |
en |
| dc.contributor.author |
Vontas, J |
en |
| dc.date.accessioned |
2014-06-06T06:49:26Z |
|
| dc.date.available |
2014-06-06T06:49:26Z |
|
| dc.date.issued |
2009 |
en |
| dc.identifier.issn |
09621075 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1111/j.1365-2583.2009.00900.x |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/4592 |
|
| dc.subject |
Bifenthrin |
en |
| dc.subject |
Inheritance of resistance |
en |
| dc.subject |
Kdr mutations |
en |
| dc.subject |
Pyrethroids |
en |
| dc.subject |
Tetranychus urticae |
en |
| dc.subject |
Voltage-gated sodium channel |
en |
| dc.subject.other |
bifenthrin |
en |
| dc.subject.other |
butyl phosphorotrithioate |
en |
| dc.subject.other |
messenger RNA |
en |
| dc.subject.other |
phosphorothioic acid derivative |
en |
| dc.subject.other |
piperonyl butoxide |
en |
| dc.subject.other |
pyrethroid |
en |
| dc.subject.other |
sodium channel |
en |
| dc.subject.other |
alternative RNA splicing |
en |
| dc.subject.other |
amino acid sequence |
en |
| dc.subject.other |
animal |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
chemistry |
en |
| dc.subject.other |
cross breeding |
en |
| dc.subject.other |
drug effect |
en |
| dc.subject.other |
female |
en |
| dc.subject.other |
gene |
en |
| dc.subject.other |
genetics |
en |
| dc.subject.other |
inheritance |
en |
| dc.subject.other |
insecticide resistance |
en |
| dc.subject.other |
male |
en |
| dc.subject.other |
metabolism |
en |
| dc.subject.other |
molecular cloning |
en |
| dc.subject.other |
molecular genetics |
en |
| dc.subject.other |
mutation |
en |
| dc.subject.other |
nucleotide sequence |
en |
| dc.subject.other |
phylogeny |
en |
| dc.subject.other |
sequence alignment |
en |
| dc.subject.other |
survival |
en |
| dc.subject.other |
Tetranychidae |
en |
| dc.subject.other |
Alternative Splicing |
en |
| dc.subject.other |
Amino Acid Sequence |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Cloning, Molecular |
en |
| dc.subject.other |
Crosses, Genetic |
en |
| dc.subject.other |
Female |
en |
| dc.subject.other |
Genes, Insect |
en |
| dc.subject.other |
Inheritance Patterns |
en |
| dc.subject.other |
Insecticide Resistance |
en |
| dc.subject.other |
Male |
en |
| dc.subject.other |
Molecular Sequence Data |
en |
| dc.subject.other |
Mutation |
en |
| dc.subject.other |
Phylogeny |
en |
| dc.subject.other |
Piperonyl Butoxide |
en |
| dc.subject.other |
Pyrethrins |
en |
| dc.subject.other |
RNA, Messenger |
en |
| dc.subject.other |
Sequence Alignment |
en |
| dc.subject.other |
Sodium Channels |
en |
| dc.subject.other |
Survival Analysis |
en |
| dc.subject.other |
Tetranychidae |
en |
| dc.subject.other |
Thiophosphoric Acid Esters |
en |
| dc.subject.other |
Acari |
en |
| dc.subject.other |
Arachnida |
en |
| dc.subject.other |
Araneae |
en |
| dc.subject.other |
Psoroptes cervinus |
en |
| dc.subject.other |
Sarcoptes scabiei |
en |
| dc.subject.other |
Tetranychidae |
en |
| dc.subject.other |
Tetranychus urticae |
en |
| dc.title |
Identification of pyrethroid resistance associated mutations in the para sodium channel of the two-spotted spider mite Tetranychus urticae (Acari: Tetranychidae) |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1111/j.1365-2583.2009.00900.x |
en |
| heal.publicationDate |
2009 |
en |
| heal.abstract |
We investigated pyrethroid resistance mechanisms in Tetranychus urticae strains from Greece. Combined bioassay, biochemical and synergistic data indicated that although P450 mono-oxygenase activities were associated with the trait, target site insensitivity was the major resistance component. A 3.3 kb cDNA fragment of the T. urticae para sodium channel gene encompassing segment 4 of domain II to segment 6 of domain IV was obtained by a degenerate PCR strategy. The T. urticae sequence showed highest identity (56%) to the scabies mite, Sarcoptes scabiei, and was phylogenetically classified within the divergent group of Arachnida. Comparison of resistant and susceptible strains identified the point mutation F1538I in segment 6 of domain III, which is known to confer strong resistance to pyrethroids, along with a second mutation (A1215D) in the intracellular linker connecting domains II and III with an unknown role. Three transcripts were identified corresponding to the k and l alternative exons. The mode of inheritance of resistance was confirmed as incompletely recessive, which is consistent with a target site mechanism for pyrethroids. © 2009 The Royal Entomological Society. |
en |
| heal.journalName |
Insect Molecular Biology |
en |
| dc.identifier.issue |
5 |
en |
| dc.identifier.volume |
18 |
en |
| dc.identifier.doi |
10.1111/j.1365-2583.2009.00900.x |
en |
| dc.identifier.spage |
583 |
en |
| dc.identifier.epage |
593 |
en |