Evaluating renewable carbon sources as substrates for single cell oil production by Cunninghamella echinulata and Mortierella isabellina

dc.contributor.author	Fakas, S	en
dc.contributor.author	Papanikolaou, S	en
dc.contributor.author	Batsos, A	en
dc.contributor.author	Galiotou-Panayotou, M	en
dc.contributor.author	Mallouchos, A	en
dc.contributor.author	Aggelis, G	en
dc.date.accessioned	2014-06-06T06:49:23Z
dc.date.available	2014-06-06T06:49:23Z
dc.date.issued	2009	en
dc.identifier.issn	09619534	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.biombioe.2008.09.006	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4567
dc.subject	γ-Linolenic acid	en
dc.subject	Cunninghamella echinulata	en
dc.subject	Mortierella isabellina	en
dc.subject	Raw glycerol	en
dc.subject	Single cell oil	en
dc.subject	Xylose	en
dc.subject.other	Acids	en
dc.subject.other	Biological materials	en
dc.subject.other	Biomass	en
dc.subject.other	Fatty acids	en
dc.subject.other	Glucose	en
dc.subject.other	Glycerol	en
dc.subject.other	Microorganisms	en
dc.subject.other	Biomass productions	en
dc.subject.other	Carbon sources	en
dc.subject.other	Cunninghamella echinulata	en
dc.subject.other	Dry matters	en
dc.subject.other	Fatty acid compositions	en
dc.subject.other	Metabolic pathways	en
dc.subject.other	Mortierella isabellina	en
dc.subject.other	Raw glycerol	en
dc.subject.other	Renewable carbon sources	en
dc.subject.other	Single cell oil	en
dc.subject.other	Storage lipids	en
dc.subject.other	Substrate uptakes	en
dc.subject.other	Total biomass	en
dc.subject.other	Total lipids	en
dc.subject.other	Substrates	en
dc.subject.other	biochemistry	en
dc.subject.other	biomass	en
dc.subject.other	cultivation	en
dc.subject.other	fungus	en
dc.subject.other	growth response	en
dc.subject.other	metabolism	en
dc.subject.other	oil production	en
dc.subject.other	renewable resource	en
dc.subject.other	substrate	en
dc.subject.other	Cunninghamella echinulata	en
dc.subject.other	Fungi	en
dc.subject.other	Mortierella	en
dc.subject.other	Mucorales	en
dc.subject.other	Umbelopsis isabellina	en
dc.title	Evaluating renewable carbon sources as substrates for single cell oil production by Cunninghamella echinulata and Mortierella isabellina	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.biombioe.2008.09.006	en
heal.publicationDate	2009	en
heal.abstract	The biochemical behavior (biomass production, accumulation of total lipid, substrate uptake, fatty acid composition of fungal oil) of two oleaginous Mucorales strains, namely Mortierella isabellina ATHUM 2935 and Cunninghamella echinulata ATHUM 4411, was studied when the aforementioned microorganisms were cultivated on xylose, raw glycerol and glucose under nitrogen-limited conditions. Significant differences in the process of lipid accumulation as related to the carbon sources used were observed for both microorganisms. These differences were attributed to the different metabolic pathways involved in the assimilation of the above substrates. Therefore, the various carbon sources were channeled, at different extent, to storage lipid or to lipid-free biomass formation. Although glucose containing media favored the production of mycelial mass (15 g L-1 of total biomass in the case of C. echinulata and 27 g L-1 in the case of M. isabellina), the accumulated lipid in dry matter was 46.0% for C. echinulata and 44.6% for M. isabellina. Lipid accumulation was induced on xylose containing media (M. isabellina accumulated 65.5% and C. echinulata 57.7% of lipid, wt wt-1, in dry mycelial mass). In these conditions, lipids of C. echinulata contained significant quantities of γ-linolenic acid (GLA). This fungus, when cultivated on xylose, produced 6.7 g L-1 of single cell oil and 1119 mg L-1 of GLA. Finally, the growth of both C. echinulata and M. isabellina on raw glycerol resulted in lower yields in terms of both biomass and oil produced than the growth on xylose. © 2008 Elsevier Ltd. All rights reserved.	en
heal.journalName	Biomass and Bioenergy	en
dc.identifier.issue	4	en
dc.identifier.volume	33	en
dc.identifier.doi	10.1016/j.biombioe.2008.09.006	en
dc.identifier.spage	573	en
dc.identifier.epage	580	en