| dc.contributor.author |
Satakarni, M |
en |
| dc.contributor.author |
Koutinas, AA |
en |
| dc.contributor.author |
Webb, C |
en |
| dc.contributor.author |
Curtis, R |
en |
| dc.date.accessioned |
2014-06-06T06:49:23Z |
|
| dc.date.available |
2014-06-06T06:49:23Z |
|
| dc.date.issued |
2009 |
en |
| dc.identifier.issn |
00063592 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1002/bit.22114 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/4563 |
|
| dc.subject |
Fusion protein |
en |
| dc.subject |
SUMO |
en |
| dc.subject |
Wheat-based biorefinery |
en |
| dc.subject.other |
Bacterial proteins |
en |
| dc.subject.other |
Complex medias |
en |
| dc.subject.other |
Expression vectors |
en |
| dc.subject.other |
Fermentation medias |
en |
| dc.subject.other |
Fusion partners |
en |
| dc.subject.other |
Fusion protein |
en |
| dc.subject.other |
Peptide fusions |
en |
| dc.subject.other |
Self-assembling peptides |
en |
| dc.subject.other |
Shake-flask cultures |
en |
| dc.subject.other |
SUMO |
en |
| dc.subject.other |
Ubiquitin |
en |
| dc.subject.other |
Wheat-based biorefinery |
en |
| dc.subject.other |
Amines |
en |
| dc.subject.other |
Biochemical engineering |
en |
| dc.subject.other |
Bioconversion |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
Fermentation |
en |
| dc.subject.other |
Glucose |
en |
| dc.subject.other |
Proteins |
en |
| dc.subject.other |
Grain (agricultural product) |
en |
| dc.subject.other |
bacterial protein |
en |
| dc.subject.other |
EAK16 SUMO 1 fusion protein |
en |
| dc.subject.other |
glucose |
en |
| dc.subject.other |
gluten |
en |
| dc.subject.other |
hexahistidine |
en |
| dc.subject.other |
hybrid protein |
en |
| dc.subject.other |
plasmid vector |
en |
| dc.subject.other |
protein hydrolysate |
en |
| dc.subject.other |
unclassified drug |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
Aspergillus awamori |
en |
| dc.subject.other |
biomass fermentation |
en |
| dc.subject.other |
bioreactor |
en |
| dc.subject.other |
cellular distribution |
en |
| dc.subject.other |
cost |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
expression vector |
en |
| dc.subject.other |
fermentation medium |
en |
| dc.subject.other |
fermentation optimization |
en |
| dc.subject.other |
fungus |
en |
| dc.subject.other |
protein expression |
en |
| dc.subject.other |
protein synthesis |
en |
| dc.subject.other |
wheat |
en |
| dc.subject.other |
Bioreactors |
en |
| dc.subject.other |
Culture Media |
en |
| dc.subject.other |
Electrophoresis, Polyacrylamide Gel |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
Exopeptidases |
en |
| dc.subject.other |
Fermentation |
en |
| dc.subject.other |
Glucose |
en |
| dc.subject.other |
Isopropyl Thiogalactoside |
en |
| dc.subject.other |
Nitrogen |
en |
| dc.subject.other |
Oligopeptides |
en |
| dc.subject.other |
Recombinant Fusion Proteins |
en |
| dc.subject.other |
SUMO-1 Protein |
en |
| dc.subject.other |
Triticum |
en |
| dc.subject.other |
Bacteria (microorganisms) |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
Triticum aestivum |
en |
| dc.title |
Enrichment of fermentation media and optimization of expression conditions for the production of EAK16 peptide as fusions with sumo |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1002/bit.22114 |
en |
| heal.publicationDate |
2009 |
en |
| heal.abstract |
EAK16 (AEAEAKAKAEAKAEAK) belongs to a novel class of self-assembling peptides, which is being investigated in research and industry. SUMO belongs to the ubiquitin class of proteins and is a promising fusion partner currently in use. In this study, EAK16 peptide fusions with hexa-histidine tagged SUMO have been constructed using Escherichia coli based pET expression vector. Intracellular expression of the SUMO-EAK16 fusion using LB media has been optimized. Low-cost complex media (fungal autolysates, wheat and gluten hydrolysates) produced via a novel wheat-based biorefinery have been used as alternative fermentation media to LB. Shake flask cultures using either enriched LB or complex wheat-derived media containing 2 g/L of glucose resulted in intracellular SUMO-EAK16 fusion protein production of approximately 250 mg/L fermentation volume which corresponded to 30-35% of the total bacterial protein expressed being the fusion protein. Fusion protein productivities up to five times higher were achieved when using a bioreactor. © 2008 Wiley Periodicals, Inc. |
en |
| heal.journalName |
Biotechnology and Bioengineering |
en |
| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
102 |
en |
| dc.identifier.doi |
10.1002/bit.22114 |
en |
| dc.identifier.spage |
725 |
en |
| dc.identifier.epage |
735 |
en |