Efficacy evaluation of a neem (Azadirachta indica A. Juss) formulation against root-knot nematodes Meloidogyne incognita

dc.contributor.author	Ntalli, NG	en
dc.contributor.author	Menkissoglu-Spiroudi, U	en
dc.contributor.author	Giannakou, IO	en
dc.contributor.author	Prophetou-Athanasiadou, DA	en
dc.date.accessioned	2014-06-06T06:49:22Z
dc.date.available	2014-06-06T06:49:22Z
dc.date.issued	2009	en
dc.identifier.issn	02612194	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.cropro.2009.01.011	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4556
dc.subject	Azadirachtin	en
dc.subject	AzaNema	en
dc.subject	HPLC	en
dc.subject	Motility	en
dc.subject	Root-knot nematodes	en
dc.subject.other	bioassay	en
dc.subject.other	biocontrol agent	en
dc.subject.other	experimental study	en
dc.subject.other	insecticide	en
dc.subject.other	juvenile	en
dc.subject.other	liquid chromatography	en
dc.subject.other	mortality	en
dc.subject.other	neem	en
dc.subject.other	nematode	en
dc.subject.other	pest control	en
dc.subject.other	Azadirachta indica	en
dc.subject.other	Meloidogyne	en
dc.subject.other	Meloidogyne incognita	en
dc.subject.other	Meloidogyne javanica	en
dc.subject.other	Nematoda	en
dc.title	Efficacy evaluation of a neem (Azadirachta indica A. Juss) formulation against root-knot nematodes Meloidogyne incognita	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.cropro.2009.01.011	en
heal.publicationDate	2009	en
heal.abstract	The nematicidal-nematostatic activity of AzaNema, 1% azadirachtin, against Meloidogyne incognita was evaluated both with motility and pot bioassays at different rates equal or greater than that recommended for nematode control (6 L ha-1). To avoid issues that could have interfered with efficacy, such as formulation inconsistency and storage instability, technical grade azadirachtin 11.8% was used to verify the test product's active ingredient (azadirachtin A) and to study the direct motility effect of azadirachtin on second stage juveniles (J2). Finally, the stability of both azadirachtin formulation types was studied under experimental conditions. Azadirachtin did not provide adequate control of nematodes, at the recommended or higher doses. In pot bioassays, a compensatory effect was achieved with 30.72 μg a.i g-1 of soil but, in general, one application per biological cycle failed to protect the plants from Meloidogyne incognita. In motility experiments, only exposure of J2 to doses higher than 12.8 μg a.i ml-1 resulted in a significant increase of immotile J2 for both AzaNema and azadirachtin technical grade. Nevertheless, the test product was found, after HPLC analysis, consistent with active ingredient content. Moreover, azadirachtin A concentration remained stable during the experiments, regardless of the formulation type tested. In conclusion, further research will be necessary to improve the efficacy of azadirachtin against Meloidogyne incognita. © 2009 Elsevier Ltd. All rights reserved.	en
heal.journalName	Crop Protection	en
dc.identifier.issue	6	en
dc.identifier.volume	28	en
dc.identifier.doi	10.1016/j.cropro.2009.01.011	en
dc.identifier.spage	489	en
dc.identifier.epage	494	en