Effects of soil application of olive mill wastewaters on the structure and function of the community of arbuscular mycorrhizal fungi

dc.contributor.author	Ipsilantis, I	en
dc.contributor.author	Karpouzas, DG	en
dc.contributor.author	Papadopoulou, KK	en
dc.contributor.author	Ehaliotis, C	en
dc.date.accessioned	2014-06-06T06:49:22Z
dc.date.available	2014-06-06T06:49:22Z
dc.date.issued	2009	en
dc.identifier.issn	00380717	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.soilbio.2009.09.003	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4553
dc.subject	Arbuscular mycorrhizal fungi	en
dc.subject	DGGE	en
dc.subject	Olive mill wastewater	en
dc.subject	Vicia faba	en
dc.subject.other	Agricultural soils	en
dc.subject.other	Am fungus	en
dc.subject.other	Arbuscular mycorrhizal fungi	en
dc.subject.other	Cloning and sequencing	en
dc.subject.other	DGGE	en
dc.subject.other	Dose levels	en
dc.subject.other	Fungal colonization	en
dc.subject.other	Fungal community	en
dc.subject.other	Fungal diversity	en
dc.subject.other	Green manures	en
dc.subject.other	Growth response	en
dc.subject.other	In-plants	en
dc.subject.other	Indirect effects	en
dc.subject.other	Olive mill wastewater	en
dc.subject.other	Olive mill wastewaters	en
dc.subject.other	Phytoxicity	en
dc.subject.other	Plant growth	en
dc.subject.other	Plant growth promoting	en
dc.subject.other	Root colonization	en
dc.subject.other	Root dry weight	en
dc.subject.other	Short-term effects	en
dc.subject.other	Soil applications	en
dc.subject.other	Soil fertility	en
dc.subject.other	Soil plant systems	en
dc.subject.other	Transient changes	en
dc.subject.other	Transient effect	en
dc.subject.other	Tree plantations	en
dc.subject.other	Vicia faba	en
dc.subject.other	Cloning	en
dc.subject.other	Forestry	en
dc.subject.other	Fungi	en
dc.subject.other	Manures	en
dc.subject.other	Plant life extension	en
dc.subject.other	Railroad tunnels	en
dc.subject.other	Soils	en
dc.subject.other	Wastewater	en
dc.subject.other	Wastewater treatment	en
dc.subject.other	Amplitude modulation	en
dc.subject.other	agricultural soil	en
dc.subject.other	arbuscular mycorrhiza	en
dc.subject.other	community structure	en
dc.subject.other	ecosystem function	en
dc.subject.other	ecotoxicology	en
dc.subject.other	fungus	en
dc.subject.other	germination	en
dc.subject.other	growth response	en
dc.subject.other	industrial waste	en
dc.subject.other	inhibition	en
dc.subject.other	legume	en
dc.subject.other	manure	en
dc.subject.other	mill	en
dc.subject.other	nutrient availability	en
dc.subject.other	pollution effect	en
dc.subject.other	recycling	en
dc.subject.other	root colonization	en
dc.subject.other	soil nutrient	en
dc.subject.other	soil-vegetation interaction	en
dc.subject.other	wastewater	en
dc.subject.other	Clones	en
dc.subject.other	Effluent Treatment	en
dc.subject.other	Forestry	en
dc.subject.other	Fungi	en
dc.subject.other	Manure	en
dc.subject.other	Railroads	en
dc.subject.other	Soil	en
dc.subject.other	Tunnels	en
dc.subject.other	Waste Waters	en
dc.subject.other	Arbuscular	en
dc.subject.other	Fungi	en
dc.subject.other	Glomus	en
dc.subject.other	Vicia faba	en
dc.title	Effects of soil application of olive mill wastewaters on the structure and function of the community of arbuscular mycorrhizal fungi	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.soilbio.2009.09.003	en
heal.publicationDate	2009	en
heal.abstract	Recycling of olive mill wastewaters (OMW) into agricultural soils is a controversial issue since benefits to soil fertility should counterbalance potential short-term toxicity effects. We investigated the short-term effects of OMW on the soil-plant system, regarding the diversity, structure and root colonization capacity of arbuscular mycorrhizal (AM) fungi and the respective growth response of Vicia faba L, commonly used as green manure in olive-tree plantations. A compartmentalized pot system was used that allowed the establishment of an AM fungal community in one compartment (feeder) and the application of three OMW dose levels in an adjacent second compartment (receiver). At 0, 10, and 30 days after OMW treatment (DAT), V. faba pre-germinated seeds were seeded in the receiver compartment. At harvest, shoot and root dry weights, AM fungal root colonization, soil hyphal length and P availability were recorded in the receiver compartment. In addition, OMW effects on AM fungal diversity in plant roots were studied by DGGE. A transient effect of OMW application was observed; plant growth and AM fungal colonization were initially inhibited, whereas soil hyphal length was stimulated, but in most cases differences were absent when seeding was performed 30 DAT. Similarly, changes induced in the structure of the root AM fungal community were of transient nature. Cloning and sequencing of all the major DGGE bands showed that roots were colonized by Glomus spp. The transient effects of OMW on the structure and function of AM fungi could be attributed to OMW-derived phytoxicity to V. faba plants or to an indirect effect via alteration of soil nutritional status. The high OMW dose significantly increased soil P availability in the presence of AM fungi, suggesting efficient involvement of AM fungi in organic-P minerilization. Overall our results indicate that soil application of OMW would cause transient changes in the AM fungal colonization of V. faba plants, which, would not impair their long-term plant growth promoting ability. © 2009 Elsevier Ltd. All rights reserved.	en
heal.journalName	Soil Biology and Biochemistry	en
dc.identifier.issue	12	en
dc.identifier.volume	41	en
dc.identifier.doi	10.1016/j.soilbio.2009.09.003	en
dc.identifier.spage	2466	en
dc.identifier.epage	2476	en