| dc.contributor.author |
Mountzouris, KC |
en |
| dc.contributor.author |
Kotzampassi, K |
en |
| dc.contributor.author |
Tsirtsikos, P |
en |
| dc.contributor.author |
Kapoutzis, K |
en |
| dc.contributor.author |
Fegeros, K |
en |
| dc.date.accessioned |
2014-06-06T06:49:21Z |
|
| dc.date.available |
2014-06-06T06:49:21Z |
|
| dc.date.issued |
2009 |
en |
| dc.identifier.issn |
02615614 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.clnu.2009.01.009 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/4549 |
|
| dc.subject |
Enzymes |
en |
| dc.subject |
Fasting |
en |
| dc.subject |
Fermentation |
en |
| dc.subject |
Microflora |
en |
| dc.subject |
Probiotics |
en |
| dc.subject |
Rats |
en |
| dc.subject.other |
acetic acid |
en |
| dc.subject.other |
alpha galactosidase |
en |
| dc.subject.other |
alpha glucosidase |
en |
| dc.subject.other |
arylsulfatase |
en |
| dc.subject.other |
azo reductase |
en |
| dc.subject.other |
bacterial enzyme |
en |
| dc.subject.other |
beta galactosidase |
en |
| dc.subject.other |
beta glucosidase |
en |
| dc.subject.other |
biological marker |
en |
| dc.subject.other |
butyric acid |
en |
| dc.subject.other |
glycocholic acid |
en |
| dc.subject.other |
hydrolase |
en |
| dc.subject.other |
probiotic agent |
en |
| dc.subject.other |
volatile fatty acid |
en |
| dc.subject.other |
animal experiment |
en |
| dc.subject.other |
animal tissue |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
cecum |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
diet restriction |
en |
| dc.subject.other |
enzyme activity |
en |
| dc.subject.other |
feeding |
en |
| dc.subject.other |
fermentation |
en |
| dc.subject.other |
intestine flora |
en |
| dc.subject.other |
intestine function |
en |
| dc.subject.other |
Lactobacillus acidophilus |
en |
| dc.subject.other |
male |
en |
| dc.subject.other |
microbial metabolism |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
nutrition |
en |
| dc.subject.other |
rat |
en |
| dc.subject.other |
refeeding |
en |
| dc.subject.other |
Wistar rat |
en |
| dc.subject.other |
alpha-Galactosidase |
en |
| dc.subject.other |
alpha-Glucosidases |
en |
| dc.subject.other |
Amidohydrolases |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
beta-Galactosidase |
en |
| dc.subject.other |
beta-Glucosidase |
en |
| dc.subject.other |
Biological Markers |
en |
| dc.subject.other |
Cecum |
en |
| dc.subject.other |
Colon |
en |
| dc.subject.other |
Fasting |
en |
| dc.subject.other |
Fatty Acids, Volatile |
en |
| dc.subject.other |
Fermentation |
en |
| dc.subject.other |
Lactobacillus acidophilus |
en |
| dc.subject.other |
Male |
en |
| dc.subject.other |
NADH, NADPH Oxidoreductases |
en |
| dc.subject.other |
Probiotics |
en |
| dc.subject.other |
Random Allocation |
en |
| dc.subject.other |
Rats |
en |
| dc.subject.other |
Rats, Wistar |
en |
| dc.title |
Effects of Lactobacillus acidophilus on gut microflora metabolic biomarkers in fed and fasted rats |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.clnu.2009.01.009 |
en |
| heal.publicationDate |
2009 |
en |
| heal.abstract |
Background & aims: Little is known about fasting effects on gut bacterial metabolism. As probiotics are purported to be beneficial for health, this study aimed to investigate the response of gut microbial metabolism on fasting with or without probiotic administration. Methods: Sixty male adult Wistar rats were allocated to six experimental treatments, for 6 days, arranged under three nutritional schemes namely: (a) ad libitum feeding (control), (b) fasting for 3 days and re-feeding for the remainder (re-fed) and (c) fasting for 6 days combined with parenteral liquid treatment during the last 3 days (starved). Each nutritional scheme had one non-probiotic and one probiotic treatment receiving orally Lactobacillus acidophilus. Rat caecal digesta were analyzed for bacterial enzyme activities and volatile fatty acids (VFA). Results: Fasted rats had significantly lower activities of α-galactosidase, α-glucosidase and β-glucosidase and higher activities of β-galactosidase and azoreductase compared to control and re-fed rats, irrespective of probiotic administration. Results were variable regarding cholylglycine hydrolase (CGH), while there were no differences between treatments regarding β-glucuronidase and arylsulfatase activity. Fasted rats had significantly lower caecal VFA concentration and different fermentation patterns. L. acidophilus resulted in significantly reduced azoreductase activity and increased caecal acetate levels in fasted rats. Re-feeding appeared to restore most enzyme activities, fermentation intensity and to some extent fermentation patterns at control treatment levels. L. acidophilus resulted in significantly reduced CGH activity and increased butyrate levels in re-fed rats. Conclusion: The results indicate a health beneficial potential of L. acidophilus in fasted and re-fed nutritional states via reduction of harmful azoreductase and CGH activities and promotion of useful VFA components for colonic function and health. © 2009 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. |
en |
| heal.journalName |
Clinical Nutrition |
en |
| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
28 |
en |
| dc.identifier.doi |
10.1016/j.clnu.2009.01.009 |
en |
| dc.identifier.spage |
318 |
en |
| dc.identifier.epage |
324 |
en |