Cloning and functional characterization of the ovine malic enzyme promoter

dc.contributor.author	Stefos, GC	en
dc.contributor.author	Becker, W	en
dc.contributor.author	Lampidonis, AD	en
dc.contributor.author	Rogdakis, E	en
dc.date.accessioned	2014-06-06T06:49:15Z
dc.date.available	2014-06-06T06:49:15Z
dc.date.issued	2009	en
dc.identifier.issn	03781119	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.gene.2008.09.027	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4504
dc.subject	Adipose	en
dc.subject	Lipogenesis	en
dc.subject	Ruminants	en
dc.subject	Sheep	en
dc.subject	T3RE	en
dc.subject.other	liothyronine	en
dc.subject.other	luciferase	en
dc.subject.other	malate dehydrogenase (decarboxylating)	en
dc.subject.other	nucleotide	en
dc.subject.other	adipocyte	en
dc.subject.other	animal cell	en
dc.subject.other	article	en
dc.subject.other	bovids	en
dc.subject.other	cell differentiation	en
dc.subject.other	cell line	en
dc.subject.other	CpG island	en
dc.subject.other	DNA flanking region	en
dc.subject.other	DNA responsive element	en
dc.subject.other	embryo	en
dc.subject.other	enzyme activity	en
dc.subject.other	GC rich sequence	en
dc.subject.other	gene deletion	en
dc.subject.other	gene expression	en
dc.subject.other	genetic transcription	en
dc.subject.other	hepatoma cell	en
dc.subject.other	human	en
dc.subject.other	human cell	en
dc.subject.other	lipogenesis	en
dc.subject.other	molecular cloning	en
dc.subject.other	mouse	en
dc.subject.other	nonhuman	en
dc.subject.other	nucleotide sequence	en
dc.subject.other	priority journal	en
dc.subject.other	promoter region	en
dc.subject.other	sheep	en
dc.subject.other	TATA box	en
dc.subject.other	Adipose Tissue	en
dc.subject.other	Animals	en
dc.subject.other	Base Sequence	en
dc.subject.other	Carcinoma, Hepatocellular	en
dc.subject.other	Cells, Cultured	en
dc.subject.other	Cloning, Molecular	en
dc.subject.other	Gene Expression Regulation, Enzymologic	en
dc.subject.other	Humans	en
dc.subject.other	Liver Neoplasms	en
dc.subject.other	Luciferases	en
dc.subject.other	Malate Dehydrogenase	en
dc.subject.other	Mice	en
dc.subject.other	Molecular Sequence Data	en
dc.subject.other	Promoter Regions, Genetic	en
dc.subject.other	Response Elements	en
dc.subject.other	Sequence Deletion	en
dc.subject.other	Sequence Homology, Nucleic Acid	en
dc.subject.other	Sheep	en
dc.subject.other	Species Specificity	en
dc.subject.other	Transcription, Genetic	en
dc.subject.other	Transfection	en
dc.subject.other	Triiodothyronine	en
dc.subject.other	Ovis	en
dc.subject.other	Ovis aries	en
dc.subject.other	Rodentia	en
dc.title	Cloning and functional characterization of the ovine malic enzyme promoter	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.gene.2008.09.027	en
heal.publicationDate	2009	en
heal.abstract	While in human and rodents lipogenesis occurs predominantly in the liver, adipose tissue is the major site in ruminants. Here we report the nucleotide sequence of the 5′-flanking region of the ovine malic enzyme gene (ME1). The ME1 promoter is located within a GC-rich region fulfilling the criteria of CpG islands and lacks a TATA-box. Deletion analysis identified a region (-231/-170) that suppressed promoter activity in luciferase assays in HepG2 hepatoma cells but not in 3T3-L1 adipocytes. This region contains a putative triiodothyronine response element (T3RE) that differs from the human ME1 T3RE by two nucleotides. When the human ME1 T3RE was introduced into the ovine ME1 promoter context, transcriptional activity was increased in the hepatic cell lines HepG2 and H4IIE but not in differentiated 3T3-L1 cells. Our results suggest that the sequence of the T3RE in the ME1 promoter determines differences in the tissue/species activity of malic enzyme in ruminants and human. © 2008 Elsevier B.V. All rights reserved.	en
heal.journalName	Gene	en
dc.identifier.issue	1-2	en
dc.identifier.volume	428	en
dc.identifier.doi	10.1016/j.gene.2008.09.027	en
dc.identifier.spage	36	en
dc.identifier.epage	40	en