Biotechnological conversions of bio-dieselderived crude glycerol by Yarrowia lipolytica strains

dc.contributor.author	Andre, A	en
dc.contributor.author	Chatzifragkou, A	en
dc.contributor.author	Diamantopoulou, P	en
dc.contributor.author	Sarris, D	en
dc.contributor.author	Philippoussis, A	en
dc.contributor.author	Galiotou-Panayotou, M	en
dc.contributor.author	Komaitis, M	en
dc.contributor.author	Papanikolaou, S	en
dc.date.accessioned	2014-06-06T06:49:12Z
dc.date.available	2014-06-06T06:49:12Z
dc.date.issued	2009	en
dc.identifier.issn	16180240	en
dc.identifier.uri	http://dx.doi.org/10.1002/elsc.200900063	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4492
dc.subject	Citric acid	en
dc.subject	Crude glycerol	en
dc.subject	Mannitol	en
dc.subject	Single cell oil	en
dc.subject	Yarrowia lipolytica	en
dc.subject.other	Carbon substrates	en
dc.subject.other	Cellular lipid	en
dc.subject.other	Concentration of	en
dc.subject.other	Crude glycerol	en
dc.subject.other	Culture conditions	en
dc.subject.other	Dry weight	en
dc.subject.other	Early growth	en
dc.subject.other	Glycerol concentration	en
dc.subject.other	Lipid concentration	en
dc.subject.other	Metabolic products	en
dc.subject.other	Microbial growth	en
dc.subject.other	Microbial lipids	en
dc.subject.other	Neutral fractions	en
dc.subject.other	Neutral lipid	en
dc.subject.other	Saturated fatty acid	en
dc.subject.other	Shake-flask experiments	en
dc.subject.other	Single cell oil	en
dc.subject.other	Stationary phase	en
dc.subject.other	Storage lipids	en
dc.subject.other	Y. lipolytica	en
dc.subject.other	Yarrowia lipolytica	en
dc.subject.other	Acids	en
dc.subject.other	Adhesive joints	en
dc.subject.other	Cell culture	en
dc.subject.other	Citric acid	en
dc.subject.other	Concentration (process)	en
dc.subject.other	Fatty acids	en
dc.subject.other	Nitrogen	en
dc.subject.other	Phospholipids	en
dc.subject.other	Glycerol	en
dc.subject.other	biodiesel	en
dc.subject.other	citric acid	en
dc.subject.other	fat	en
dc.subject.other	glycerol	en
dc.subject.other	lipid	en
dc.subject.other	mannitol	en
dc.subject.other	saturated fatty acid	en
dc.subject.other	alcohol	en
dc.subject.other	bioengineering	en
dc.subject.other	biofuel	en
dc.subject.other	biotransformation	en
dc.subject.other	citric acid	en
dc.subject.other	experimental study	en
dc.subject.other	fungus	en
dc.subject.other	industrial waste	en
dc.subject.other	lipid	en
dc.subject.other	metabolite	en
dc.subject.other	microbial activity	en
dc.subject.other	substrate	en
dc.subject.other	article	en
dc.subject.other	biotechnology	en
dc.subject.other	culture medium	en
dc.subject.other	fungal strain	en
dc.subject.other	fungus growth	en
dc.subject.other	industrial waste	en
dc.subject.other	nonhuman	en
dc.subject.other	Yarrowia lipolytica	en
dc.subject.other	Yarrowia lipolytica	en
dc.title	Biotechnological conversions of bio-dieselderived crude glycerol by Yarrowia lipolytica strains	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/elsc.200900063	en
heal.publicationDate	2009	en
heal.abstract	In the present report, crude glycerol, waste discharged from bio-diesel production, was used as carbon substrate for three natural Yarrowia lipolytica strains (LFMB 19, LFMB 20 and ACA-YC 5033) during growth in nitrogen-limited submerged shake-flask experiments. In media with initial glycerol concentration of 30 g/L, all strains presented satisfactory microbial growth and complete glycerol uptake. Although culture conditions favored the secretion of citric acid (and potentially the accumulation of storage lipid), for the strains LFMB 19 and LFMB 20, polyol mannitol was the principal metabolic product synthesized (maximum quantity 6.0 g/L, yield 0.20-0.26 g per g of glycerol consumed). The above strains produced small quantities of lipids and citric acid. In contrast, Y. lipolytica ACA-YC 5033 produced simultaneously higher quantities of lipid and citric acid and was further grown on crude glycerol in nitrogen-limited experiments, with constant nitrogen and increasing glycerol concentrations (70-120 g/L). Citric acid and lipid concentrations increased with increment of glycerol; maximum total citric acid 50.1 g/L was produced (yield 0.44 g per g of glycerol) while simultaneously 2.0 g/L of fat were accumulated inside the cells (0.31 g of lipid per g of dry weight). Cellular lipids were mainly composed of neutral fraction, the concentration of which substantially increased with time. Moreover, in any case, the phospholipid fraction was more unsaturated compared with total and neutral lipids, while at the early growth step, microbial lipid was more rich in saturated fatty acids (e.g. C16:0 and C18:0) compared with the stationary phase. © 2009 Wiley-VCH Verlag GmbH & Co.	en
heal.journalName	Engineering in Life Sciences	en
dc.identifier.issue	6	en
dc.identifier.volume	9	en
dc.identifier.doi	10.1002/elsc.200900063	en
dc.identifier.spage	468	en
dc.identifier.epage	478	en