| dc.contributor.author |
Kanakis, CD |
en |
| dc.contributor.author |
Tarantilis, PA |
en |
| dc.contributor.author |
Pappas, C |
en |
| dc.contributor.author |
Bariyanga, J |
en |
| dc.contributor.author |
Tajmir-Riahi, HA |
en |
| dc.contributor.author |
Polissiou, MG |
en |
| dc.date.accessioned |
2014-06-06T06:49:11Z |
|
| dc.date.available |
2014-06-06T06:49:11Z |
|
| dc.date.issued |
2009 |
en |
| dc.identifier.issn |
10111344 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.jphotobiol.2009.03.006 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/4481 |
|
| dc.subject |
Antioxidant |
en |
| dc.subject |
Conformation |
en |
| dc.subject |
DNA |
en |
| dc.subject |
FT-IR |
en |
| dc.subject |
Saffron |
en |
| dc.subject |
tRNA |
en |
| dc.subject.other |
1,1 diphenyl 2 picrylhydrazyl |
en |
| dc.subject.other |
butylcresol |
en |
| dc.subject.other |
crocetin |
en |
| dc.subject.other |
Crocus sativus extract |
en |
| dc.subject.other |
dimethylcrocetin |
en |
| dc.subject.other |
DNA |
en |
| dc.subject.other |
RNA |
en |
| dc.subject.other |
safranal |
en |
| dc.subject.other |
transfer RNA |
en |
| dc.subject.other |
trolox C |
en |
| dc.subject.other |
unclassified drug |
en |
| dc.subject.other |
antineoplastic activity |
en |
| dc.subject.other |
antioxidant activity |
en |
| dc.subject.other |
aqueous solution |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
binding kinetics |
en |
| dc.subject.other |
cattle |
en |
| dc.subject.other |
concentration (parameters) |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
Crocus |
en |
| dc.subject.other |
crocus sativus |
en |
| dc.subject.other |
DNA adduct |
en |
| dc.subject.other |
DNA structure |
en |
| dc.subject.other |
flower |
en |
| dc.subject.other |
IC 50 |
en |
| dc.subject.other |
priority journal |
en |
| dc.subject.other |
protein conformation |
en |
| dc.subject.other |
RNA structure |
en |
| dc.subject.other |
spectroscopy |
en |
| dc.subject.other |
spice |
en |
| dc.subject.other |
stigma |
en |
| dc.subject.other |
thymus |
en |
| dc.subject.other |
traditional medicine |
en |
| dc.subject.other |
Antioxidants |
en |
| dc.subject.other |
Carotenoids |
en |
| dc.subject.other |
Crocus |
en |
| dc.subject.other |
Cyclohexenes |
en |
| dc.subject.other |
DNA |
en |
| dc.subject.other |
DNA Adducts |
en |
| dc.subject.other |
Models, Chemical |
en |
| dc.subject.other |
Nucleic Acid Conformation |
en |
| dc.subject.other |
RNA, Transfer |
en |
| dc.subject.other |
Spectroscopy, Fourier Transform Infrared |
en |
| dc.subject.other |
Terpenes |
en |
| dc.subject.other |
Crocus sativus |
en |
| dc.title |
An overview of structural features of DNA and RNA complexes with saffron compounds: Models and antioxidant activity |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.jphotobiol.2009.03.006 |
en |
| heal.publicationDate |
2009 |
en |
| heal.abstract |
Saffron is the red dried stigmas of Crocus sativus L. flowers and used both as a spice and as a drug in traditional medicine. Its numerous applications as an antioxidant and anticancer agent are due to its secondary metabolites and their derivatives (safranal, crocetin, dimethylcrocetin). In this work we are comparing the spectroscopic results and antioxidant activities of saffron components safranal, crocetin (CRT) and dimethylcrocetin (DMCRT) complexes with calf-thymus DNA (ctDNA) and transfer RNA (tRNA) in aqueous solution at physiological conditions Intercalative and external binding modes of saffron compounds to DNA and RNA were observed with overall binding constants of Ksafranal = 1.24 × 103 M-1, KCRT = 6.20 × 103 M-1 and KDMCRT = 1.85 × 105 M-1, for DNA adducts and Ksafranal = 6.80 × 103 M-1, KCRT = 1.40 × 104 M-1 and KDMCRT = 3.40 × 104 M-1 for RNA complexes. A partial B- to A-DNA transition occurred at high ligand concentrations, while tRNA remained in A-conformation in saffron-RNA complexes. The antioxidant activity of CRT, DMCRT and safranal was also tested by the DPPḢ (2,2-diphenyl-1-picrylhydrazyl) antioxidant activity assay and their IC50 values were compared to that of well known antioxidants such as Trolox and Butylated Hydroxy Toluene (BHT). The IC50 values were 95 ± 1 μg/mL for safranal and 18 ± 1 μg/mL for crocetin. The inhibition of DMCRT reached a point of 38.8%, which corresponds to a concentration of 40 μg/mL. © 2009 Elsevier B.V. All rights reserved. |
en |
| heal.journalName |
Journal of Photochemistry and Photobiology B: Biology |
en |
| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
95 |
en |
| dc.identifier.doi |
10.1016/j.jphotobiol.2009.03.006 |
en |
| dc.identifier.spage |
204 |
en |
| dc.identifier.epage |
212 |
en |