A cytosolic invertase is required for normal growth and cell development in the model legume, Lotus japonicus

dc.contributor.author	Welham, T	en
dc.contributor.author	Pike, J	en
dc.contributor.author	Horst, I	en
dc.contributor.author	Flemetakis, E	en
dc.contributor.author	Katinakis, P	en
dc.contributor.author	Kaneko, T	en
dc.contributor.author	Sato, S	en
dc.contributor.author	Tabata, S	en
dc.contributor.author	Perry, J	en
dc.contributor.author	Parniske, M	en
dc.contributor.author	Wang, TL	en
dc.date.accessioned	2014-06-06T06:49:10Z
dc.date.available	2014-06-06T06:49:10Z
dc.date.issued	2009	en
dc.identifier.issn	00220957	en
dc.identifier.uri	http://dx.doi.org/10.1093/jxb/erp169	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4474
dc.subject	Cellular development	en
dc.subject	Legume	en
dc.subject	Lotus japonicus	en
dc.subject	Mutants	en
dc.subject	Neutral/alkaline invertase	en
dc.subject	Plant development	en
dc.subject	Sucrose metabolism	en
dc.subject	TILLING	en
dc.subject.other	beta fructofuranosidase	en
dc.subject.other	isoprotein	en
dc.subject.other	vegetable protein	en
dc.subject.other	article	en
dc.subject.other	biological model	en
dc.subject.other	cytosol	en
dc.subject.other	enzymology	en
dc.subject.other	genetics	en
dc.subject.other	growth, development and aging	en
dc.subject.other	legume	en
dc.subject.other	Lotus	en
dc.subject.other	metabolism	en
dc.subject.other	nodulation	en
dc.subject.other	beta-Fructofuranosidase	en
dc.subject.other	Cytosol	en
dc.subject.other	Fabaceae	en
dc.subject.other	Lotus	en
dc.subject.other	Models, Biological	en
dc.subject.other	Plant Proteins	en
dc.subject.other	Protein Isoforms	en
dc.subject.other	Root Nodules, Plant	en
dc.subject.other	Cyanobacteria	en
dc.subject.other	Lotus corniculatus var. japonicus	en
dc.title	A cytosolic invertase is required for normal growth and cell development in the model legume, Lotus japonicus	en
heal.type	journalArticle	en
heal.identifier.primary	10.1093/jxb/erp169	en
heal.publicationDate	2009	en
heal.abstract	Neutral/alkaline invertases are a subgroup, confined to plants and cyanobacteria, of a diverse family of enzymes. A family of seven closely-related genes, LjINV1-LjINV7, is described here and their expression in the model legume, Lotus japonicus, is examined. LjINV1 previously identified as encoding a nodule-enhanced isoform is the predominant isoform present in all parts of the plant. Mutants for two isoforms, LjINV1 and LjINV2, were isolated using TILLING. A premature stop codon allele of LjINV2 had no effect on enzyme activity nor did it show a visible phenotype. For LjINV1, premature stop codon and missense mutations were obtained and the phenotype of the mutants examined. Recovery of homozygous mutants was problematic, but their phenotype showed a severe reduction in growth of the root and the shoot, a change in cellular development, and impaired flowering. The cellular organization of both roots and leaves was altered; leaves were smaller and thicker with extra layers of cells and roots showed an extended and broader zone of cell division. Moreover, anthers contained no pollen. Both heterozygotes and homozygous mutants showed decreased amounts of enzyme activity in nodules and shoot tips. Shoot tips also contained up to a 9-fold increased level of sucrose. However, mutants were capable of forming functional root nodules. LjINV1 is therefore crucial to whole plant development, but is clearly not essential for nodule formation or function.	en
heal.journalName	Journal of Experimental Botany	en
dc.identifier.issue	12	en
dc.identifier.volume	60	en
dc.identifier.doi	10.1093/jxb/erp169	en
dc.identifier.spage	3353	en
dc.identifier.epage	3365	en