| dc.contributor.author |
Lents, CA |
en |
| dc.contributor.author |
Farmerie, TA |
en |
| dc.contributor.author |
Cherrington, BD |
en |
| dc.contributor.author |
Clay, CM |
en |
| dc.date.accessioned |
2014-06-06T06:49:00Z |
|
| dc.date.available |
2014-06-06T06:49:00Z |
|
| dc.date.issued |
2009 |
en |
| dc.identifier.issn |
1355008X |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1007/s12020-009-9167-1 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/4374 |
|
| dc.subject |
Gene regulation |
en |
| dc.subject |
GnRH receptor |
en |
| dc.subject |
Homeodomain |
en |
| dc.subject |
Pituitary |
en |
| dc.subject |
Transcription |
en |
| dc.subject.other |
gonadorelin receptor |
en |
| dc.subject.other |
homeodomain protein |
en |
| dc.subject.other |
octamer transcription factor 1 |
en |
| dc.subject.other |
gonadorelin receptor |
en |
| dc.subject.other |
octamer transcription factor 1 |
en |
| dc.subject.other |
animal cell |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
binding site |
en |
| dc.subject.other |
DNA binding |
en |
| dc.subject.other |
DNA binding motif |
en |
| dc.subject.other |
gene sequence |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
priority journal |
en |
| dc.subject.other |
promoter region |
en |
| dc.subject.other |
site directed mutagenesis |
en |
| dc.subject.other |
transcription initiation |
en |
| dc.subject.other |
transcription regulation |
en |
| dc.subject.other |
animal |
en |
| dc.subject.other |
cell culture |
en |
| dc.subject.other |
gene expression regulation |
en |
| dc.subject.other |
genetics |
en |
| dc.subject.other |
metabolism |
en |
| dc.subject.other |
molecular genetics |
en |
| dc.subject.other |
mouse |
en |
| dc.subject.other |
nucleotide sequence |
en |
| dc.subject.other |
physiology |
en |
| dc.subject.other |
promoter region |
en |
| dc.subject.other |
protein binding |
en |
| dc.subject.other |
protein domain |
en |
| dc.subject.other |
protein motif |
en |
| dc.subject.other |
protein multimerization |
en |
| dc.subject.other |
transcription initiation |
en |
| dc.subject.other |
Amino Acid Motifs |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Base Sequence |
en |
| dc.subject.other |
Binding Sites |
en |
| dc.subject.other |
Cells, Cultured |
en |
| dc.subject.other |
Gene Expression Regulation, Developmental |
en |
| dc.subject.other |
Homeodomain Proteins |
en |
| dc.subject.other |
Mice |
en |
| dc.subject.other |
Molecular Sequence Data |
en |
| dc.subject.other |
Octamer Transcription Factor-1 |
en |
| dc.subject.other |
Promoter Regions, Genetic |
en |
| dc.subject.other |
Protein Binding |
en |
| dc.subject.other |
Protein Interaction Domains and Motifs |
en |
| dc.subject.other |
Protein Multimerization |
en |
| dc.subject.other |
Receptors, LHRH |
en |
| dc.subject.other |
Transcriptional Activation |
en |
| dc.title |
Multiple core homeodomain binding motifs differentially contribute to transcriptional activity of the murine gonadotropin-releasing hormone receptor gene promoter |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1007/s12020-009-9167-1 |
en |
| heal.publicationDate |
2009 |
en |
| heal.abstract |
Multiple homeodomain (Hbox) proteins have been shown to organize expression of key markers of gonadotropes. Nine putative Hbox-binding sites, characterized by the homeospecific TAAT motif, are located within the proximal 600 bp of the murine GnRHR promoter. Homeoproteins bind separate Hbox sites within this promoter, supporting basal- and endocrine-directed transcription. The function of the most proximal sites (Hbox1 and Hbox2) in the murine GnRHR is unknown; thus, understanding of the global contribution of homeospecific TAAT sites to promoter function is incomplete. Site-directed mutagenesis revealed that loss of Hbox2 reduced promoter activity in a cell-specific manner, having no effect in αT3-1 cells but reducing promoter function in LβT2 cells, another gonadotrope-derived cell line representing a later developmental stage. In contrast, eliminating Hbox1 reduced basal activity in both lines. This region displayed specific binding to homeoprotein Oct-1. Mutagenesis of a previously identified Oct-1-binding site in concert with Hbox1 led to further reduction in activity. We suggest that the two most proximal homeodomain-binding sites in the murine GnRHR promoter may regulate the promoter in a developmentally dependent fashion and that Oct-1 acts at multiple but distinct TAAT sites to support basal transcription. © 2009 Humana Press. |
en |
| heal.journalName |
Endocrine |
en |
| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
35 |
en |
| dc.identifier.doi |
10.1007/s12020-009-9167-1 |
en |
| dc.identifier.spage |
356 |
en |
| dc.identifier.epage |
364 |
en |