| dc.contributor.author |
Scherzer, J |
en |
| dc.contributor.author |
Fayrer-Hosken, RA |
en |
| dc.contributor.author |
Aceves, M |
en |
| dc.contributor.author |
Hurley, DJ |
en |
| dc.contributor.author |
Ray, LE |
en |
| dc.contributor.author |
Jones, L |
en |
| dc.contributor.author |
Heusner, GL |
en |
| dc.date.accessioned |
2014-06-06T06:48:57Z |
|
| dc.date.available |
2014-06-06T06:48:57Z |
|
| dc.date.issued |
2009 |
en |
| dc.identifier.issn |
00050423 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1111/j.1751-0813.2009.00452.x |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/4358 |
|
| dc.subject |
Cryopreservation |
en |
| dc.subject |
Extender |
en |
| dc.subject |
Glycerol |
en |
| dc.subject |
Horses |
en |
| dc.subject |
Semen |
en |
| dc.subject.other |
cryoprotective agent |
en |
| dc.subject.other |
glycerol |
en |
| dc.subject.other |
animal |
en |
| dc.subject.other |
animal disease |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
comparative study |
en |
| dc.subject.other |
cryopreservation |
en |
| dc.subject.other |
horse |
en |
| dc.subject.other |
male |
en |
| dc.subject.other |
methodology |
en |
| dc.subject.other |
physiology |
en |
| dc.subject.other |
randomization |
en |
| dc.subject.other |
sperm |
en |
| dc.subject.other |
sperm preservation |
en |
| dc.subject.other |
spermatozoon motility |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Cryopreservation |
en |
| dc.subject.other |
Cryoprotective Agents |
en |
| dc.subject.other |
Glycerol |
en |
| dc.subject.other |
Horses |
en |
| dc.subject.other |
Male |
en |
| dc.subject.other |
Random Allocation |
en |
| dc.subject.other |
Semen |
en |
| dc.subject.other |
Semen Preservation |
en |
| dc.subject.other |
Sperm Motility |
en |
| dc.subject.other |
Equidae |
en |
| dc.title |
Freezing equine semen: The effect of combinations of semen extenders and glycerol on post-thaw motility |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1111/j.1751-0813.2009.00452.x |
en |
| heal.publicationDate |
2009 |
en |
| heal.abstract |
Objective We evaluated combinations of two commercial semen extenders and three concentrations of glycerol to determine the combination that yielded the highest post-thaw sperm motility. Design A randomised 2 × 3 block design was used. Procedure Semen was collected from four stallions (6 collections per stallion). The sample was diluted with either a dried skim-milk glucose extender (EZ Mixin Original Formula) or a chemically defined, milk-free diluent (INRA 96), and each was used in combination with 2%, 3% or 4% glycerol in standard commercial freezing medium. Sperm motility was assessed by microscopy in fresh and post-thaw semen. Results There was a significant difference between the two extenders in the motility of spermatozoa after cryopreservation (48.9% for INRA 96; 38.6% for EZ Mixin OF; P < 0.0001). Glycerol at 4% in freezing medium yielded the highest post-thaw motility, significantly better than 2% (P < 0.05). Three of four stallions had significantly higher post-thaw motility using INRA 96 relative to EZ Mixin OF (P < 0.01), and two of four stallions had significantly higher post-thaw motility using 4% glycerol (P < 0.05). The combination of INRA 96 and 4% glycerol in freezing medium gave the highest average post-thaw motility of 51.5%. Conclusion In this study, INRA 96 combined with 4% glycerol yielded an average recovery of progressively motile sperm consistently above the 35% target. © 2009 Australian Veterinary Association. |
en |
| heal.journalName |
Australian Veterinary Journal |
en |
| dc.identifier.issue |
7 |
en |
| dc.identifier.volume |
87 |
en |
| dc.identifier.doi |
10.1111/j.1751-0813.2009.00452.x |
en |
| dc.identifier.spage |
275 |
en |
| dc.identifier.epage |
279 |
en |