| dc.contributor.author |
Kamnev, AA |
en |
| dc.contributor.author |
Sadovnikova, JN |
en |
| dc.contributor.author |
Tarantilis, PA |
en |
| dc.contributor.author |
Polissiou, MG |
en |
| dc.contributor.author |
Antonyuk, LP |
en |
| dc.date.accessioned |
2014-06-06T06:48:51Z |
|
| dc.date.available |
2014-06-06T06:48:51Z |
|
| dc.date.issued |
2008 |
en |
| dc.identifier.issn |
00953628 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1007/s00248-008-9381-z |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/4301 |
|
| dc.subject.other |
hydroxybutyric acid |
en |
| dc.subject.other |
nitrogen |
en |
| dc.subject.other |
poly(3 hydroxybutyric acid) |
en |
| dc.subject.other |
poly-beta-hydroxybutyrate |
en |
| dc.subject.other |
polyester |
en |
| dc.subject.other |
wheat germ agglutinin |
en |
| dc.subject.other |
aerobic metabolism |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
Azospirillum brasilense |
en |
| dc.subject.other |
drug effect |
en |
| dc.subject.other |
growth, development and aging |
en |
| dc.subject.other |
infrared spectroscopy |
en |
| dc.subject.other |
metabolism |
en |
| dc.subject.other |
methodology |
en |
| dc.subject.other |
Aerobiosis |
en |
| dc.subject.other |
Azospirillum brasilense |
en |
| dc.subject.other |
Hydroxybutyrates |
en |
| dc.subject.other |
Nitrogen |
en |
| dc.subject.other |
Polyesters |
en |
| dc.subject.other |
Spectroscopy, Fourier Transform Infrared |
en |
| dc.subject.other |
Wheat Germ Agglutinins |
en |
| dc.subject.other |
Azospirillum brasilense |
en |
| dc.subject.other |
Bacteria (microorganisms) |
en |
| dc.subject.other |
Rhizobium |
en |
| dc.subject.other |
Triticum aestivum |
en |
| dc.title |
Responses of Azospirillum brasilense to nitrogen deficiency and to wheat lectin: A diffuse reflectance infrared fourier transform (DRIFT) spectroscopic study |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1007/s00248-008-9381-z |
en |
| heal.publicationDate |
2008 |
en |
| heal.abstract |
For the rhizobacterium Azospirillum brasilense, the optimal nutritional range of C:N ratios corresponds to the presence of malate (ca. 3 to 5 g l -1 of its sodium salt) and ammonium (ca. 0.5 to 3 g l -1 of NH 4Cl) as preferred carbon and nitrogen sources, respectively. This microaerophilic aerotactic bacterium is known to have a narrow optimal oxygen concentration range of ca. 3 to 5 μM, which is 1.2% to 2% of oxygen solubility in air-saturated water under normal conditions. In this work, the effects of stress conditions (bound-nitrogen deficiency related to a high C:N ratio in the medium; excess of oxygen) on aerobically grown A. brasilense Sp245, a native wheat-associated endophyte, were investigated in the absence and presence of wheat germ agglutinin (WGA, plant stress protein and a molecular host-plant signal for the bacterium) using FTIR spectroscopy of whole cells in the diffuse reflectance mode (DRIFT). The nutritional stress resulted in the appearance of prominent spectroscopic signs of poly-3-hydroxybutyrate (PHB) accumulation in the bacterial cells; in addition, splitting of the amide I band related to bacterial cellular proteins indicated some stress-induced alterations in their secondary structure components. Similar structural changes were observed in the presence of nanomolar WGA both in stressed A. brasilense cells and under normal nutritional conditions. Comparative analysis of the data obtained and the relevant literature data indicated that the stress conditions applied (which resulted in the accumulation of PHB involved in stress tolerance) and/or the presence of nanomolar concentrations of WGA induced synthesis of bacterial cell-surface (glyco)proteins rich in β-structures, that could be represented by hemagglutinin and/or porin. © 2008 Springer Science+Business Media, LLC. |
en |
| heal.journalName |
Microbial Ecology |
en |
| dc.identifier.issue |
4 |
en |
| dc.identifier.volume |
56 |
en |
| dc.identifier.doi |
10.1007/s00248-008-9381-z |
en |
| dc.identifier.spage |
615 |
en |
| dc.identifier.epage |
624 |
en |