| dc.contributor.author |
Minioti, KS |
en |
| dc.contributor.author |
Georgiou, CA |
en |
| dc.date.accessioned |
2014-06-06T06:48:50Z |
|
| dc.date.available |
2014-06-06T06:48:50Z |
|
| dc.date.issued |
2008 |
en |
| dc.identifier.issn |
03088146 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.foodchem.2007.12.077 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/4291 |
|
| dc.subject |
Flow injection |
en |
| dc.subject |
Food quality control |
en |
| dc.subject |
Olive oil |
en |
| dc.subject |
Total antioxidant capacity |
en |
| dc.subject.other |
4 iodophenol |
en |
| dc.subject.other |
antioxidant |
en |
| dc.subject.other |
gallic acid |
en |
| dc.subject.other |
horseradish peroxidase |
en |
| dc.subject.other |
hydrogen peroxide |
en |
| dc.subject.other |
luminol |
en |
| dc.subject.other |
methanol |
en |
| dc.subject.other |
olive oil |
en |
| dc.subject.other |
phenol derivative |
en |
| dc.subject.other |
phosphate |
en |
| dc.subject.other |
unclassified drug |
en |
| dc.subject.other |
water |
en |
| dc.subject.other |
accuracy |
en |
| dc.subject.other |
antioxidant activity |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
automation |
en |
| dc.subject.other |
bioluminescence |
en |
| dc.subject.other |
calculation |
en |
| dc.subject.other |
concentration response |
en |
| dc.subject.other |
cultivar |
en |
| dc.subject.other |
flow injection analysis |
en |
| dc.subject.other |
flow rate |
en |
| dc.subject.other |
food quality |
en |
| dc.subject.other |
high throughput screening |
en |
| dc.subject.other |
hydrophilicity |
en |
| dc.subject.other |
infrared radiation |
en |
| dc.subject.other |
oxidation |
en |
| dc.subject.other |
quality control |
en |
| dc.subject.other |
Armoracia rusticana |
en |
| dc.title |
High throughput flow injection bioluminometric method for olive oil antioxidant capacity |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.foodchem.2007.12.077 |
en |
| heal.publicationDate |
2008 |
en |
| heal.abstract |
This paper describes a rapid flow injection automated method for the determination of olive oil total antioxidant capacity. The chemistry involved is the horseradish peroxidase (HRP) catalysed oxidation of luminol by hydrogen peroxide. Oxidation results in light emission (bioluminescence) that is enhanced using p-iodophenol sensitizer. Olive oil (0.7 mL) is extracted with two 0.7 mL aliquots of 80-20% (v/v) methanol-water solvent. A 17 μL aliquot of the extract containing hydrophilic antioxidants is injected in a phosphate buffer channel that subsequently merges with a luminol-HRP-p-iodophenol reagent stream. Bioluminescence resulting after merging the mixture with a hydrogen peroxide stream is suppressed upon increasing antioxidants' concentration resulting in negative peaks due to hydrogen peroxide consumption by antioxidants. The method has been optimized on (a) number of manifold channels, (b) flow rates, (c) coil length and (d) HRP, hydrogen peroxide and p-iodophenol concentrations. Detection limit is calculated at 1.5 × 10-7 M gallic acid, linear range is between 1.0 × 10-6 and 1 × 10-4 M and precision is better than 2.8% RSD (n = 4). The fully automated method is achieving a rate of sampling equal 180 probes per hour. The proposed method is applied for the assessment of 50 extra-virgin olive oil samples of different Greek cultivars and regions. © 2008 Elsevier Ltd. All rights reserved. |
en |
| heal.journalName |
Food Chemistry |
en |
| dc.identifier.issue |
2 |
en |
| dc.identifier.volume |
109 |
en |
| dc.identifier.doi |
10.1016/j.foodchem.2007.12.077 |
en |
| dc.identifier.spage |
455 |
en |
| dc.identifier.epage |
461 |
en |