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Genetic diversity analysis of Mediterranean faba bean (Vicia faba L.) with ISSR markers

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dc.contributor.author Terzopoulos, PJ en
dc.contributor.author Bebeli, PJ en
dc.date.accessioned 2014-06-06T06:48:50Z
dc.date.available 2014-06-06T06:48:50Z
dc.date.issued 2008 en
dc.identifier.issn 03784290 en
dc.identifier.uri http://dx.doi.org/10.1016/j.fcr.2008.02.015 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/4288
dc.subject Faba bean en
dc.subject Gene pool en
dc.subject Inter-Simple Sequence Repeat en
dc.subject Mediterranean en
dc.subject Synthetic varieties en
dc.subject.other cluster analysis en
dc.subject.other comparative study en
dc.subject.other genetic marker en
dc.subject.other genetic variation en
dc.subject.other germplasm en
dc.subject.other legume en
dc.subject.other Mediterranean environment en
dc.subject.other morphology en
dc.subject.other polymorphism en
dc.subject.other population genetics en
dc.subject.other Eurasia en
dc.subject.other Europe en
dc.subject.other Greece en
dc.subject.other Southern Europe en
dc.subject.other Vicia faba en
dc.title Genetic diversity analysis of Mediterranean faba bean (Vicia faba L.) with ISSR markers en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.fcr.2008.02.015 en
heal.publicationDate 2008 en
heal.abstract Crosses among inbred lines from different Mediterranean-type faba bean gene pools appear promising for the production of high yielding synthetic varieties. The purpose of the present study was to (1) describe the genetic diversity of Greek faba bean local populations, using Inter-Simple Sequence Repeats (ISSRs), and (2) classify the local populations and compare the classification results with those derived from morphological/agronomic data. Four ISSR primers were used on 57 bulked samples of 20 faba bean local populations, five minor-type and 15 Mediterranean-type populations. A total of 192 DNA bands were obtained, of which 190 were polymorphic. Analysis of molecular variance (AMOVA) revealed high levels of within population genetic variation. Based on Nei's standard genetic distances, both cluster analysis using unweighted pair-group method with arithmetic mean (UPGMA) and principal coordinate analysis (PCoA) separated four minor-type populations from the Mediterranean-type populations. The Mediterranean-type populations were further classified into two clusters, with the resultant groups being less diverse than the initial Mediterranean-type gene pool. Mantel test showed that the ISSR markers classification using Nei's genetic distances was statistically different from that derived from morphological/agronomic data using Manhattan distances. Conclusively, the present study supported the suggestion that Mediterranean-type faba beans can be subdivided into at least two different germplasm pools. The groups studied are promising for the production of synthetic varieties. © 2008 Elsevier B.V. All rights reserved. en
heal.journalName Field Crops Research en
dc.identifier.issue 1 en
dc.identifier.volume 108 en
dc.identifier.doi 10.1016/j.fcr.2008.02.015 en
dc.identifier.spage 39 en
dc.identifier.epage 44 en


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