γ-Linolenic acid production by Cunninghamella echinulata growing on complex organic nitrogen sources

dc.contributor.author	Fakas, S	en
dc.contributor.author	Certik, M	en
dc.contributor.author	Papanikolaou, S	en
dc.contributor.author	Aggelis, G	en
dc.contributor.author	Komaitis, M	en
dc.contributor.author	Galiotou-Panayotou, M	en
dc.date.accessioned	2014-06-06T06:48:47Z
dc.date.available	2014-06-06T06:48:47Z
dc.date.issued	2008	en
dc.identifier.issn	09608524	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.biortech.2007.10.016	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4274
dc.subject	GLA production	en
dc.subject	Lipid fractions	en
dc.subject	Proteases	en
dc.subject	Tomato waste	en
dc.subject.other	Biomass	en
dc.subject.other	Catalyst activity	en
dc.subject.other	Lipids	en
dc.subject.other	Nitrogen	en
dc.subject.other	GLA production	en
dc.subject.other	Lipid fractions	en
dc.subject.other	Tomato waste	en
dc.subject.other	Organic acids	en
dc.subject.other	gamma linolenic acid	en
dc.subject.other	glucose	en
dc.subject.other	gluten	en
dc.subject.other	glycolipid	en
dc.subject.other	lipid	en
dc.subject.other	organic nitrogen	en
dc.subject.other	phospholipid	en
dc.subject.other	protein hydrolysate	en
dc.subject.other	sphingolipid	en
dc.subject.other	Biomass	en
dc.subject.other	Catalyst activity	en
dc.subject.other	Lipids	en
dc.subject.other	Nitrogen	en
dc.subject.other	Organic acids	en
dc.subject.other	biomass	en
dc.subject.other	enzyme activity	en
dc.subject.other	fatty acid	en
dc.subject.other	fruit	en
dc.subject.other	fungus	en
dc.subject.other	glucose	en
dc.subject.other	growth rate	en
dc.subject.other	lipid	en
dc.subject.other	organic nitrogen	en
dc.subject.other	article	en
dc.subject.other	biomass	en
dc.subject.other	biosynthesis	en
dc.subject.other	corn	en
dc.subject.other	Cunninghamella echinulata	en
dc.subject.other	fatty acid synthesis	en
dc.subject.other	fungus growth	en
dc.subject.other	mycelium	en
dc.subject.other	priority journal	en
dc.subject.other	protein degradation	en
dc.subject.other	quantitative analysis	en
dc.subject.other	tomato	en
dc.subject.other	whey	en
dc.subject.other	yeast	en
dc.subject.other	Bioreactors	en
dc.subject.other	Culture Media	en
dc.subject.other	Cunninghamella	en
dc.subject.other	Fermentation	en
dc.subject.other	Fungal Proteins	en
dc.subject.other	gamma-Linolenic Acid	en
dc.subject.other	Lycopersicon esculentum	en
dc.subject.other	Nitrogen	en
dc.subject.other	Organic Chemicals	en
dc.subject.other	Peptide Hydrolases	en
dc.subject.other	Cunninghamella echinulata	en
dc.subject.other	Lycopersicon esculentum	en
dc.subject.other	Zea mays	en
dc.title	γ-Linolenic acid production by Cunninghamella echinulata growing on complex organic nitrogen sources	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.biortech.2007.10.016	en
heal.publicationDate	2008	en
heal.abstract	Growth of two strains of Cunninghamella echinulata on various nitrogen containing raw materials (corn gluten, corn steep, whey concentrate, yeast extract and tomato waste hydrolysate) yielded important amounts of biomass containing various quantities of γ-linolenic acid (GLA) rich cellular lipids. Especially, growth on tomato waste hydrolysate (TWH) yielded 17.6 g/l of biomass containing 39.6% oil and significant quantities of GLA corresponding to 800 mg/l GLA. Mycelium-bounded proteolytic activity was detected during early growth stages on TWH and declined thereafter, increasing the concentration of assimilable nitrogen in the medium. However, addition of glucose in the medium during the stationary phase triggered the biosynthesis of reserve lipid, since an increase of the proportion of neutral lipids from 45% to 79% in total lipids was observed, while polar lipids decreased from 35% to 12% and from 20% to 9% for glycolipids plus sphingolipids and phospholipids, respectively. © 2007 Elsevier Ltd. All rights reserved.	en
heal.journalName	Bioresource Technology	en
dc.identifier.issue	13	en
dc.identifier.volume	99	en
dc.identifier.doi	10.1016/j.biortech.2007.10.016	en
dc.identifier.spage	5986	en
dc.identifier.epage	5990	en