| dc.contributor.author |
Rogato, A |
en |
| dc.contributor.author |
D'Apuzzo, E |
en |
| dc.contributor.author |
Barbulova, A |
en |
| dc.contributor.author |
Omrane, S |
en |
| dc.contributor.author |
Stedel, C |
en |
| dc.contributor.author |
Simon-Rosin, U |
en |
| dc.contributor.author |
Katinakis, P |
en |
| dc.contributor.author |
Flemetakis, M |
en |
| dc.contributor.author |
Udvardi, M |
en |
| dc.contributor.author |
Chiurazzi, M |
en |
| dc.date.accessioned |
2014-06-06T06:48:47Z |
|
| dc.date.available |
2014-06-06T06:48:47Z |
|
| dc.date.issued |
2008 |
en |
| dc.identifier.issn |
01674412 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1007/s11103-008-9394-5 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/4267 |
|
| dc.subject |
Ammonium transport |
en |
| dc.subject |
Nitrogen fixation |
en |
| dc.subject |
Nodule |
en |
| dc.subject |
Symbiosis |
en |
| dc.subject.other |
primer DNA |
en |
| dc.subject.other |
vegetable protein |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
down regulation |
en |
| dc.subject.other |
in situ hybridization |
en |
| dc.subject.other |
Lotus |
en |
| dc.subject.other |
nitrogen fixation |
en |
| dc.subject.other |
nucleotide sequence |
en |
| dc.subject.other |
physiology |
en |
| dc.subject.other |
reverse transcription polymerase chain reaction |
en |
| dc.subject.other |
transgenic plant |
en |
| dc.subject.other |
Base Sequence |
en |
| dc.subject.other |
DNA Primers |
en |
| dc.subject.other |
Down-Regulation |
en |
| dc.subject.other |
In Situ Hybridization |
en |
| dc.subject.other |
Lotus |
en |
| dc.subject.other |
Nitrogen Fixation |
en |
| dc.subject.other |
Plant Proteins |
en |
| dc.subject.other |
Plants, Genetically Modified |
en |
| dc.subject.other |
Reverse Transcriptase Polymerase Chain Reaction |
en |
| dc.subject.other |
Lotus corniculatus var. japonicus |
en |
| dc.title |
Tissue-specific down-regulation of LjAMT1;1 compromises nodule function and enhances nodulation in Lotus japonicus |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1007/s11103-008-9394-5 |
en |
| heal.publicationDate |
2008 |
en |
| heal.abstract |
Plant ammonium transporters of the AMT1 family are involved in N-uptake from the soil and ammonium transport, and recycling within the plant. Although AMT1 genes are known to be expressed in nitrogen-fixing nodules of legumes, their precise roles in this specialized organ remain unknown. We have taken a reverse-genetic approach to decipher the physiological role of LjAMT1;1 in Lotus japonicus nodules. LjAMT1;1 is normally expressed in both the infected zone and the vascular tissue of Lotus nodules. Inhibition of LjAMT1;1 gene expression, using an antisense gene construct driven by a leghemoglobin promoter resulted in a substantial reduction of LjAMT1;1 transcript in the infected tissue but not the vascular bundles of transgenic plants. As a result, the nitrogen-fixing activity of nodules was partially impaired and nodule number increased compared to control plants. Expression of LjAMT1;1-GFP fusion protein in plant cells indicated a plasma-membrane location for the LjAMT1;1 protein. Taken together, the results are consistent with a role of LjAMT1;1 in retaining ammonium derived from symbiotic nitrogen fixation in plant cells prior to its assimilation. © 2008 The Author(s). |
en |
| heal.journalName |
Plant Molecular Biology |
en |
| dc.identifier.issue |
6 |
en |
| dc.identifier.volume |
68 |
en |
| dc.identifier.doi |
10.1007/s11103-008-9394-5 |
en |
| dc.identifier.spage |
585 |
en |
| dc.identifier.epage |
595 |
en |