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Survival of Bacillus cereus vegetative cells during Spanish-style fermentation of conservolea green olives

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dc.contributor.author Panagou, EZ en
dc.contributor.author Tassou, CC en
dc.contributor.author Vamvakoula, P en
dc.contributor.author Saravanos, EKA en
dc.contributor.author Nychas, G-JE en
dc.date.accessioned 2014-06-06T06:48:42Z
dc.date.available 2014-06-06T06:48:42Z
dc.date.issued 2008 en
dc.identifier.issn 0362028X en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/4243
dc.relation.uri http://www.scopus.com/inward/record.url?eid=2-s2.0-46949095447&partnerID=40&md5=f9ef2ae1521beee64290a5dd4d20aa1f en
dc.subject.other lactic acid en
dc.subject.other article en
dc.subject.other Bacillus cereus en
dc.subject.other bacterial count en
dc.subject.other fermentation en
dc.subject.other food contamination en
dc.subject.other food control en
dc.subject.other food handling en
dc.subject.other growth, development and aging en
dc.subject.other Lactobacillus en
dc.subject.other Lactobacillus plantarum en
dc.subject.other metabolism en
dc.subject.other methodology en
dc.subject.other microbiology en
dc.subject.other multivariate analysis en
dc.subject.other olive tree en
dc.subject.other pH en
dc.subject.other principal component analysis en
dc.subject.other safety en
dc.subject.other species difference en
dc.subject.other time en
dc.subject.other Bacillus cereus en
dc.subject.other Colony Count, Microbial en
dc.subject.other Consumer Product Safety en
dc.subject.other Fermentation en
dc.subject.other Food Contamination en
dc.subject.other Food Handling en
dc.subject.other Food Microbiology en
dc.subject.other Hydrogen-Ion Concentration en
dc.subject.other Lactic Acid en
dc.subject.other Lactobacillus en
dc.subject.other Lactobacillus plantarum en
dc.subject.other Multivariate Analysis en
dc.subject.other Olea en
dc.subject.other Principal Component Analysis en
dc.subject.other Species Specificity en
dc.subject.other Time Factors en
dc.subject.other Bacillus cereus en
dc.subject.other Lactobacillus en
dc.subject.other Lactobacillus pentosus en
dc.subject.other Lactobacillus plantarum en
dc.subject.other Oleaceae en
dc.title Survival of Bacillus cereus vegetative cells during Spanish-style fermentation of conservolea green olives en
heal.type journalArticle en
heal.publicationDate 2008 en
heal.abstract The aim of this research was to investigate the survival of artificially inoculated Bacillus cereus during Spanish-style green olive fermentation. Olives were initially treated with lye and subjected to different fermentation procedures including (i) heat shock (85°C for 10 min) and inoculation with Lactobacillus plantarum ACA-DC 287, (ii) heat shock and inoculation with L. plantarum ACA-DC 146, (iii) heat shock and inoculation with Lactobacillus pentosus isolated during previous studies, and (iv) fermentation by indigenous flora (control process). Microbial growth and survival, pH, titratable acidity, and organic acid evolution were monitored. Inactivation of B. cereus was observed during all processes. The pathogen population declined during all fermentations, but a tailing effect was observed in the brines when the population reached 2 log CFU/ml, at which point the pathogen does not pose a risk to human health. The rate of inactivation was higher in heat-shocked inoculated olives (mean of -2.21 log CFU/day) compared with control olives (-1.26 log/day), indicating an advantage of heat shock and inoculation over spontaneous fermentation. The production of organic acids (primarily lactic acid) during fermentation seemed to be the main factor that determined the behavior of the pathogen under stress conditions prevailing in the brine. Principal components analysis was useful for distinguishing among the different fermentation processes on the basis of the relevant organic acid profile. Copyright ©, International Association for Food Protection. en
heal.journalName Journal of Food Protection en
dc.identifier.issue 7 en
dc.identifier.volume 71 en
dc.identifier.spage 1393 en
dc.identifier.epage 1400 en


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