RNA arbitrarily primed PCR and fourier transform infrared spectroscopy reveal plasticity in the acid tolerance response of Streptococcus macedonicus

dc.contributor.author	Papadimitriou, K	en
dc.contributor.author	Boutou, E	en
dc.contributor.author	Zoumpopoulou, G	en
dc.contributor.author	Tarantilis, PA	en
dc.contributor.author	Polissiou, M	en
dc.contributor.author	Vorgias, CE	en
dc.contributor.author	Tsakalidou, E	en
dc.date.accessioned	2014-06-06T06:48:40Z
dc.date.available	2014-06-06T06:48:40Z
dc.date.issued	2008	en
dc.identifier.issn	00992240	en
dc.identifier.uri	http://dx.doi.org/10.1128/AEM.00315-08	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4228
dc.subject.other	ABS resins	en
dc.subject.other	Acids	en
dc.subject.other	Biopolymers	en
dc.subject.other	Cell membranes	en
dc.subject.other	Cells	en
dc.subject.other	Chemical analysis	en
dc.subject.other	Chromatographic analysis	en
dc.subject.other	Cosmic rays	en
dc.subject.other	Cytology	en
dc.subject.other	Elasticity	en
dc.subject.other	Fatty acids	en
dc.subject.other	Fourier transform infrared spectroscopy	en
dc.subject.other	Fourier transforms	en
dc.subject.other	Gene expression	en
dc.subject.other	Glycerol	en
dc.subject.other	Nucleic acids	en
dc.subject.other	Plasticity	en
dc.subject.other	Plastics	en
dc.subject.other	Polysaccharides	en
dc.subject.other	Principal component analysis	en
dc.subject.other	Programming theory	en
dc.subject.other	Proteins	en
dc.subject.other	RNA	en
dc.subject.other	Spectroscopic analysis	en
dc.subject.other	Spectrum analysis	en
dc.subject.other	Theorem proving	en
dc.subject.other	Acid adaptations	en
dc.subject.other	Acid tolerance responses	en
dc.subject.other	Acidic phs	en
dc.subject.other	Acyl carrier proteins	en
dc.subject.other	Cell walls	en
dc.subject.other	Cellular compositions	en
dc.subject.other	Cellular constituents	en
dc.subject.other	Chemical compositions	en
dc.subject.other	Diacylglycerol	en
dc.subject.other	Fourier transform infrared	en
dc.subject.other	Genes coding	en
dc.subject.other	Glucosyltransferase	en
dc.subject.other	Hypothetical proteins	en
dc.subject.other	Ir-spectra	en
dc.subject.other	Principal components	en
dc.subject.other	Spectral regions	en
dc.subject.other	Stationary phases	en
dc.subject.other	Stress conditions	en
dc.subject.other	Synthase	en
dc.subject.other	Transcriptional profiles	en
dc.subject.other	Infrared spectroscopy	en
dc.subject.other	3 oxoacyl acyl carrier protein synthase	en
dc.subject.other	fatty acid	en
dc.subject.other	polysaccharide	en
dc.subject.other	RNA	en
dc.subject.other	acid	en
dc.subject.other	bacterial DNA	en
dc.subject.other	bacterial polysaccharide	en
dc.subject.other	bacterial protein	en
dc.subject.other	bacterial RNA	en
dc.subject.other	acidity	en
dc.subject.other	adsorption	en
dc.subject.other	bacterium	en
dc.subject.other	comparative study	en
dc.subject.other	enzyme activity	en
dc.subject.other	FTIR spectroscopy	en
dc.subject.other	phenotypic plasticity	en
dc.subject.other	polymerase chain reaction	en
dc.subject.other	principal component analysis	en
dc.subject.other	protein	en
dc.subject.other	RNA	en
dc.subject.other	tolerance	en
dc.subject.other	article	en
dc.subject.other	cell membrane	en
dc.subject.other	cell wall	en
dc.subject.other	controlled study	en
dc.subject.other	gene expression	en
dc.subject.other	gene sequence	en
dc.subject.other	genetic code	en
dc.subject.other	infrared spectroscopy	en
dc.subject.other	nonhuman	en
dc.subject.other	nucleotide sequence	en
dc.subject.other	phenotype	en
dc.subject.other	polymerase chain reaction	en
dc.subject.other	RNA fingerprinting	en
dc.subject.other	Streptococcus	en
dc.subject.other	streptococcus macedonicus	en
dc.subject.other	adaptation	en
dc.subject.other	chemistry	en
dc.subject.other	DNA sequence	en
dc.subject.other	drug effect	en
dc.subject.other	gene expression profiling	en
dc.subject.other	genetics	en
dc.subject.other	methodology	en
dc.subject.other	molecular genetics	en
dc.subject.other	nucleic acid amplification	en
dc.subject.other	physiology	en
dc.subject.other	Bacteria (microorganisms)	en
dc.subject.other	Streptococcus macedonicus	en
dc.subject.other	Acids	en
dc.subject.other	Adaptation, Physiological	en
dc.subject.other	Bacterial Proteins	en
dc.subject.other	Cell Membrane	en
dc.subject.other	Cell Wall	en
dc.subject.other	DNA, Bacterial	en
dc.subject.other	Fatty Acids	en
dc.subject.other	Gene Expression Profiling	en
dc.subject.other	Molecular Sequence Data	en
dc.subject.other	Nucleic Acid Amplification Techniques	en
dc.subject.other	Polysaccharides, Bacterial	en
dc.subject.other	RNA, Bacterial	en
dc.subject.other	Sequence Analysis, DNA	en
dc.subject.other	Spectroscopy, Fourier Transform Infrared	en
dc.subject.other	Streptococcus	en
dc.title	RNA arbitrarily primed PCR and fourier transform infrared spectroscopy reveal plasticity in the acid tolerance response of Streptococcus macedonicus	en
heal.type	journalArticle	en
heal.identifier.primary	10.1128/AEM.00315-08	en
heal.publicationDate	2008	en
heal.abstract	We have previously reported that an acid tolerance response (ATR) can be induced in Streptococcus macedonicus cells at mid-log phase after autoacidification, transient exposure to acidic pH, or acid habituation, as well as at stationary phase. Here, we compared the transcriptional profiles of these epigenetic phenotypes, by RNA arbitrarily primed PCR (RAP-PCR), and their whole-cell chemical compositions, by Fourier transform infrared spectroscopy (FT-IR). RAP-PCR fingerprints revealed significant differences among the phenotypes, indicating that gene expression during the ATR is influenced not only by the growth phase but also by the treatments employed to induce the response. The genes coding for the mannose-specific IID component, the 1,2-diacylglycerol 3-glucosyltransferase, the 3-oxoacyl-acyl carrier protein, the large subunit of carbamoylphosphate synthase, and a hypothetical protein were found to be induced at least under some of the acid-adapting conditions. Furthermore, principal component analysis of the second-derivative-transformed FT-IR spectra segregated S. macedonicus phenotypes individually in all spectral regions that are characteristic for major cellular constituents like the polysaccharides of the cell wall, fatty acids of the cell membrane, proteins, and other compounds that absorb in these regions. These findings provide evidence for major changes in cellular composition due to acid adaptation that were clearly different to some extent among the phenotypes. Overall, our data demonstrate the plasticity in the ATR of S. macedonicus, which reflects the inherent ability of the bacterium to adjust the response to the distinctiveness of the imposed stress condition, probably to maximize its adaptability. Copyright © 2008, American Society for Microbiology. All Rights Reserved.	en
heal.journalName	Applied and Environmental Microbiology	en
dc.identifier.issue	19	en
dc.identifier.volume	74	en
dc.identifier.doi	10.1128/AEM.00315-08	en
dc.identifier.spage	6068	en
dc.identifier.epage	6076	en