Natural antioxidant constituents from selected aromatic plants and their antimicrobial activity against selected pathogenic microorganisms

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dc.contributor.author Proestos, C en
dc.contributor.author Boziaris, IS en
dc.contributor.author Kapsokefalou, M en
dc.contributor.author Komaitis, M en
dc.date.accessioned 2014-06-06T06:48:37Z
dc.date.available 2014-06-06T06:48:37Z
dc.date.issued 2008 en
dc.identifier.issn 13309862 en
dc.relation.uri http://www.scopus.com/inward/record.url?eid=2-s2.0-46349084913&partnerID=40&md5=5b04dd6b2538d77c8d5a5233d4935cf3 en
dc.subject Antimicrobial activity en
dc.subject GC-MS en
dc.subject Plant antioxidants en
dc.subject RP-HPLC en
dc.subject Trimethyisilyi derivatives en
dc.subject.other Anti-microbial activity en
dc.subject.other Folin-ciocalteu assays en
dc.subject.other Gas chromatography-mass spectrometry methods en
dc.subject.other GC-MS en
dc.subject.other Pathogenic microorganisms en
dc.subject.other Reversed-phase high-performance liquid chromatography en
dc.subject.other RP-HPLC en
dc.subject.other Total phenolic content en
dc.subject.other Antioxidants en
dc.subject.other Aromatic compounds en
dc.subject.other Bacteria en
dc.subject.other Gas chromatography en
dc.subject.other Plant extracts en
dc.subject.other Bacteria (microorganisms) en
dc.subject.other Crataegus en
dc.subject.other Filipendula en
dc.subject.other Filipendula ulmaria en
dc.subject.other Helianthus en
dc.subject.other Pelargonium en
dc.subject.other Polygonum en
dc.subject.other Polygonum aviculare en
dc.subject.other Potentilla en
dc.subject.other Potentilla anserina en
dc.title Natural antioxidant constituents from selected aromatic plants and their antimicrobial activity against selected pathogenic microorganisms en
heal.type conferenceItem en
heal.publicationDate 2008 en
heal.abstract Aromatic plants contain natural antioxidant constituents such as phenolic compounds, which have attracted a great deal of public and scientific interest because of their health-promoting effects as antioxidants. Five plants, Filipendula ulmaria (meadow sweet), Cratae-gus monogyna (hawthorn), Polygonum aviculare (polygonum), Potentilla anserina (silverweed), and Pelargonium purpureum (little robin), have been examined in order to determine their phenolic composition. Reversed-phase high performance liquid chromatography (RP-HPLC) was employed for the identification and quantification of phenolic compounds of the aforementioned plants. Gas chromatography-mass spectrometry method (GC-MS) was also used for identification of phenolic compounds after silylation. Analysis of the non-volatile and thermolabile phenolic compounds by GC-MS presupposes their conversion into volatile and thermotolerant derivatives. The derlvatization process was optimized against reagents, temperature and reaction time. The antioxidant capacity was determined in dried plants and in their methanol extracts with the Rancimat test using sunflower oil as substrate. Both pulverized plants and extracts showed antioxidant capacity. Total phenolic content in the extracts was determined spectrometrkally applying the Folin-Ciocalteu assay and it ranged from 7.2 to 28.2 gallic acid equivalents (GAE)/(mg/mL). Antimicrobial activity of the extracts against selected microorganisms was performed using the disk diffusion method. Gram-(+) bacteria were more sensitive to the plant extracts than Gram-(-) bacteria. en
heal.journalName Food Technology and Biotechnology en
dc.identifier.issue 2 en
dc.identifier.volume 46 en
dc.identifier.spage 151 en
dc.identifier.epage 156 en

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