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Microbiological and biochemical profile of cv. Conservolea naturally black olives during controlled fermentation with selected strains of lactic acid bacteria

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dc.contributor.author Panagou, EZ en
dc.contributor.author Schillinger, U en
dc.contributor.author Franz, CMAP en
dc.contributor.author Nychas, G-JE en
dc.date.accessioned 2014-06-06T06:48:35Z
dc.date.available 2014-06-06T06:48:35Z
dc.date.issued 2008 en
dc.identifier.issn 07400020 en
dc.identifier.uri http://dx.doi.org/10.1016/j.fm.2007.10.005 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/4199
dc.subject Conservolea en
dc.subject Fermentation en
dc.subject Lactobacillus pentosus en
dc.subject Lactobacillus plantarum en
dc.subject Naturally black olives en
dc.subject Organic acids en
dc.subject Volatile compounds en
dc.subject.other lactic acid en
dc.subject.other volatile fatty acid en
dc.subject.other article en
dc.subject.other bacterial count en
dc.subject.other fermentation en
dc.subject.other food contamination en
dc.subject.other food control en
dc.subject.other food handling en
dc.subject.other growth, development and aging en
dc.subject.other high performance liquid chromatography en
dc.subject.other Lactobacillus en
dc.subject.other Lactobacillus plantarum en
dc.subject.other metabolism en
dc.subject.other methodology en
dc.subject.other microbiology en
dc.subject.other olive tree en
dc.subject.other pH en
dc.subject.other safety en
dc.subject.other species difference en
dc.subject.other time en
dc.subject.other volatilization en
dc.subject.other Chromatography, High Pressure Liquid en
dc.subject.other Colony Count, Microbial en
dc.subject.other Consumer Product Safety en
dc.subject.other Fatty Acids, Volatile en
dc.subject.other Fermentation en
dc.subject.other Food Contamination en
dc.subject.other Food Handling en
dc.subject.other Food Microbiology en
dc.subject.other Hydrogen-Ion Concentration en
dc.subject.other Lactic Acid en
dc.subject.other Lactobacillus en
dc.subject.other Lactobacillus plantarum en
dc.subject.other Olea en
dc.subject.other Species Specificity en
dc.subject.other Time Factors en
dc.subject.other Volatilization en
dc.subject.other Bacteria (microorganisms) en
dc.subject.other Lactobacillus en
dc.subject.other Lactobacillus pentosus en
dc.subject.other Lactobacillus plantarum en
dc.subject.other Manihot esculenta en
dc.subject.other Negibacteria en
dc.subject.other Oleaceae en
dc.title Microbiological and biochemical profile of cv. Conservolea naturally black olives during controlled fermentation with selected strains of lactic acid bacteria en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.fm.2007.10.005 en
heal.publicationDate 2008 en
heal.abstract The effect of controlled fermentation processes on the microbial association and biochemical profile of cv. Conservolea naturally black olives processed by the traditional anaerobic method was studied. The different treatments included (a) inoculation with a commercial starter culture of Lactobacillus pentosus, (b) inoculation with a strain of Lactobacillus plantarum isolated from a fermented cassava product and (c) uninoculated spontaneous process. Microbial growth, pH, titratable acidity, organic acids and volatile compounds were monitored throughout the fermentation. The initial microbiota consisted of Gram-negative bacteria, lactic acid bacteria and yeasts. Inhibition of Gram-negative bacteria was evident in all processes. Both starter cultures were effective in establishing an accelerated fermentation process and reduced the survival period of Gram-negative bacteria by 5 days compared with the spontaneous process, minimizing thus the likelihood of spoilage. Higher acidification of the brines was observed in inoculated processes without any significant difference between the two selected starter cultures (113.5 and 117.6 mM for L. plantarum and L. pentosus, respectively). L. pentosus was also determined as the major species present during the whole process of spontaneous olive fermentation. It is characteristic that lactic acid fermentation was also initiated rapidly in the spontaneous process, as the conditions of fermentation, mainly the low salt level (6%, w/v) favored the dominance of lactic acid bacteria over yeasts. Lactic, acetic and propionic were the organic acids detected by HPLC in considerable amounts, whereas citric and malic acids were also present at low levels and degraded completely during the processes. Ethanol, methanol, acetaldehyde, ethyl acetate were the major volatile compounds identified by GC. Their concentrations varied among the different treatments, reflecting varying degrees of microbial activity in the brines. The results obtained from this study could help the Greek table olive industry to improve the existing processing schemes in order to increase product consistency and quality expanding the international market for naturally black olives. © 2007 Elsevier Ltd. All rights reserved. en
heal.journalName Food Microbiology en
dc.identifier.issue 2 en
dc.identifier.volume 25 en
dc.identifier.doi 10.1016/j.fm.2007.10.005 en
dc.identifier.spage 348 en
dc.identifier.epage 358 en


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