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Identification and characterization of Enterococcus spp. in greek spontaneous sausage fermentation

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dc.contributor.author Paramithiotis, S en
dc.contributor.author Kagkli, D-M en
dc.contributor.author Blana, VA en
dc.contributor.author Nychas, G-JE en
dc.contributor.author Drosinos, EH en
dc.date.accessioned 2014-06-06T06:48:31Z
dc.date.available 2014-06-06T06:48:31Z
dc.date.issued 2008 en
dc.identifier.issn 0362028X en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/4175
dc.relation.uri http://www.scopus.com/inward/record.url?eid=2-s2.0-45149127245&partnerID=40&md5=f5f1ba17d2cdfaf3cf1129f689ba29e2 en
dc.subject.other RNA 16S en
dc.subject.other animal en
dc.subject.other article en
dc.subject.other classification en
dc.subject.other Enterococcus faecium en
dc.subject.other fermentation en
dc.subject.other food control en
dc.subject.other genetic variability en
dc.subject.other genetics en
dc.subject.other Greece en
dc.subject.other growth, development and aging en
dc.subject.other human en
dc.subject.other isolation and purification en
dc.subject.other meat en
dc.subject.other microbiology en
dc.subject.other phenotype en
dc.subject.other phylogeny en
dc.subject.other pulsed field gel electrophoresis en
dc.subject.other safety en
dc.subject.other Animals en
dc.subject.other Consumer Product Safety en
dc.subject.other Electrophoresis, Gel, Pulsed-Field en
dc.subject.other Enterococcus faecium en
dc.subject.other Fermentation en
dc.subject.other Food Microbiology en
dc.subject.other Greece en
dc.subject.other Humans en
dc.subject.other Meat Products en
dc.subject.other Phenotype en
dc.subject.other Phylogeny en
dc.subject.other RNA, Ribosomal, 16S en
dc.subject.other Variation (Genetics) en
dc.subject.other Enterococcus en
dc.subject.other Enterococcus faecium en
dc.subject.other Listeria monocytogenes en
dc.title Identification and characterization of Enterococcus spp. in greek spontaneous sausage fermentation en
heal.type journalArticle en
heal.publicationDate 2008 en
heal.abstract A total of 108 enterococcal strains previously isolated from spontaneously fermented sausages were identified using phenotypic traits, pulsed-field gel electrophoresis (PFGE), and sequencing of the 16S rRNA gene. The proteolytic and lipolytic activities of these isolates and their ability to decarboxylate lysine, tyrosine, ornithine, and histidine and to produce antimicrobial compounds also were assessed. All strains were identified as Enterococcus faecium, and a lack of correlation between data derived from phenotypic and those derived from genotypic techniques was evident. Wide strain diversity was revealed by both phenotypic properties and PFGE strain typing results. Few strains were present in all batches, suggesting a possible persistence in the respective production plants. Neither proteolytic nor lipolytic activities were detected, and none of the strains decarboxylated lysine, tyrosine, ornithine, or histidine. A total of 42 E. faecium strains inhibited in vitro growth of Listeria monocytogenes, which suggests possible contribution of these strains to the safety of the end product and possible utilization of these strains as protective cultures. Copyright ©, International Association for Food Protection. en
heal.journalName Journal of Food Protection en
dc.identifier.issue 6 en
dc.identifier.volume 71 en
dc.identifier.spage 1244 en
dc.identifier.epage 1247 en


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