| dc.contributor.author |
Moschopoulou, G |
en |
| dc.contributor.author |
Vitsa, K |
en |
| dc.contributor.author |
Bem, F |
en |
| dc.contributor.author |
Vassilakos, N |
en |
| dc.contributor.author |
Perdikaris, A |
en |
| dc.contributor.author |
Blouhos, P |
en |
| dc.contributor.author |
Yialouris, C |
en |
| dc.contributor.author |
Frosyniotis, D |
en |
| dc.contributor.author |
Anthopoulos, I |
en |
| dc.contributor.author |
Mangana, O |
en |
| dc.contributor.author |
Nomikou, K |
en |
| dc.contributor.author |
Rodeva, V |
en |
| dc.contributor.author |
Kostova, D |
en |
| dc.contributor.author |
Grozeva, S |
en |
| dc.contributor.author |
Michaelides, A |
en |
| dc.contributor.author |
Simonian, A |
en |
| dc.contributor.author |
Kintzios, S |
en |
| dc.date.accessioned |
2014-06-06T06:48:27Z |
|
| dc.date.available |
2014-06-06T06:48:27Z |
|
| dc.date.issued |
2008 |
en |
| dc.identifier.issn |
09565663 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.bios.2008.06.039 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/4157 |
|
| dc.subject |
Bioelectric recognition assay |
en |
| dc.subject |
Cell biosensor |
en |
| dc.subject |
Cucumber green mottle mosaic virus |
en |
| dc.subject |
Membrane-engineering |
en |
| dc.subject |
Tobacco rattle virus |
en |
| dc.subject.other |
Bio sensor |
en |
| dc.subject.other |
Bioelectric recognition assay |
en |
| dc.subject.other |
Cell biosensor |
en |
| dc.subject.other |
Cucumber green mottle mosaic virus |
en |
| dc.subject.other |
Cucumber mosaic virus |
en |
| dc.subject.other |
Engineering processes |
en |
| dc.subject.other |
Fibroblast cells |
en |
| dc.subject.other |
Hyperpolarization |
en |
| dc.subject.other |
Membrane potentials |
en |
| dc.subject.other |
Membrane-engineering |
en |
| dc.subject.other |
Novel concept |
en |
| dc.subject.other |
Tobacco rattle virus |
en |
| dc.subject.other |
Virus detection |
en |
| dc.subject.other |
Antigens |
en |
| dc.subject.other |
Bioelectric potentials |
en |
| dc.subject.other |
Biosensors |
en |
| dc.subject.other |
Calcium |
en |
| dc.subject.other |
Cell culture |
en |
| dc.subject.other |
Cells |
en |
| dc.subject.other |
Computer viruses |
en |
| dc.subject.other |
Cytology |
en |
| dc.subject.other |
Fibroblasts |
en |
| dc.subject.other |
Fluorescence microscopy |
en |
| dc.subject.other |
Imaging techniques |
en |
| dc.subject.other |
Membranes |
en |
| dc.subject.other |
Microelectrodes |
en |
| dc.subject.other |
Microscopic examination |
en |
| dc.subject.other |
Process engineering |
en |
| dc.subject.other |
Technology |
en |
| dc.subject.other |
Tissue |
en |
| dc.subject.other |
Viruses |
en |
| dc.subject.other |
Cell membranes |
en |
| dc.subject.other |
antibody |
en |
| dc.subject.other |
animal cell |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
bioengineering |
en |
| dc.subject.other |
biosensor |
en |
| dc.subject.other |
cell contact |
en |
| dc.subject.other |
cell membrane |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
Cucumber mosaic virus |
en |
| dc.subject.other |
fibroblast |
en |
| dc.subject.other |
fluorescence microscopy |
en |
| dc.subject.other |
membrane potential |
en |
| dc.subject.other |
microelectrode |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
tobacco |
en |
| dc.subject.other |
Vero cell |
en |
| dc.subject.other |
virus detection |
en |
| dc.subject.other |
virus particle |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Antibodies, Viral |
en |
| dc.subject.other |
Biological Assay |
en |
| dc.subject.other |
Biosensing Techniques |
en |
| dc.subject.other |
Cell Membrane |
en |
| dc.subject.other |
Cercopithecus aethiops |
en |
| dc.subject.other |
Cucumovirus |
en |
| dc.subject.other |
Electrochemistry |
en |
| dc.subject.other |
Equipment Design |
en |
| dc.subject.other |
Equipment Failure Analysis |
en |
| dc.subject.other |
Genetic Engineering |
en |
| dc.subject.other |
Reproducibility of Results |
en |
| dc.subject.other |
Sensitivity and Specificity |
en |
| dc.subject.other |
Vero Cells |
en |
| dc.subject.other |
Cucumber green mottle mosaic virus |
en |
| dc.subject.other |
Cucumber mosaic virus |
en |
| dc.subject.other |
Nicotiana obtusifolia |
en |
| dc.subject.other |
Tobacco rattle virus |
en |
| dc.title |
Engineering of the membrane of fibroblast cells with virus-specific antibodies: A novel biosensor tool for virus detection |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.bios.2008.06.039 |
en |
| heal.publicationDate |
2008 |
en |
| heal.abstract |
A novel concept for the assay of viral antigens is described. The methodological approach is based on a membrane-engineering process involving the electroinsertion of virus-specific antibodies in the membranes of fibroblast cells. As a representative example, Vero fibroblasts were engineered with antibodies against Cucumber mosaic virus (CMV) and used for the construction of an ultra-sensitive miniature cell biosensor system. The attachment of a homologous virus triggered specific changes to the cell membrane potential that were measured by appropriate microelectrodes, according to the principle of the bioelectric recognition assay (BERA). No change in the membrane potential was observed upon cell contact with the heterologous cucumber green mottle mosaic virus (CGMMV). Fluorescence microscopy observations showed that attachment of CMV particles to membrane-engineered cells was associated with membrane hyperpolarization and increased [Ca2+]cyt. In an additional field-based application, we were able to detect CMV-infected tobacco plants at an essentially 100% level of accuracy. © 2008 Elsevier B.V. All rights reserved. |
en |
| heal.journalName |
Biosensors and Bioelectronics |
en |
| dc.identifier.issue |
4 |
en |
| dc.identifier.volume |
24 |
en |
| dc.identifier.doi |
10.1016/j.bios.2008.06.039 |
en |
| dc.identifier.spage |
1027 |
en |
| dc.identifier.epage |
1030 |
en |