Disinfectant test against monoculture and mixed-culture biofilms composed of technological, spoilage and pathogenic bacteria: Bactericidal effect of essential oil and hydrosol of Satureja thymbra and comparison with standard acid-base sanitizers

dc.contributor.author	Chorianopoulos, NG	en
dc.contributor.author	Giaouris, ED	en
dc.contributor.author	Skandamis, PN	en
dc.contributor.author	Haroutounian, SA	en
dc.contributor.author	Nychas, G-JE	en
dc.date.accessioned	2014-06-06T06:48:25Z
dc.date.available	2014-06-06T06:48:25Z
dc.date.issued	2008	en
dc.identifier.issn	13645072	en
dc.identifier.uri	http://dx.doi.org/10.1111/j.1365-2672.2007.03694.x	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4138
dc.subject	Antimicrobial	en
dc.subject	Biofilms	en
dc.subject	Disinfection	en
dc.subject	Essential oil	en
dc.subject	Hydrosol	en
dc.subject	Inhibitors	en
dc.subject	Satureja thymbra	en
dc.subject.other	disinfectant agent	en
dc.subject.other	essential oil	en
dc.subject.other	hydrochloric acid	en
dc.subject.other	lactic acid	en
dc.subject.other	retinol	en
dc.subject.other	sodium hydroxide	en
dc.subject.other	stainless steel	en
dc.subject.other	antimicrobial activity	en
dc.subject.other	assessment method	en
dc.subject.other	bacterium	en
dc.subject.other	biofilm	en
dc.subject.other	comparative study	en
dc.subject.other	disinfection	en
dc.subject.other	essential oil	en
dc.subject.other	herb	en
dc.subject.other	inhibitor	en
dc.subject.other	monoculture	en
dc.subject.other	pesticide	en
dc.subject.other	sanitation	en
dc.subject.other	testing method	en
dc.subject.other	antimicrobial activity	en
dc.subject.other	article	en
dc.subject.other	bactericidal activity	en
dc.subject.other	biofilm	en
dc.subject.other	controlled study	en
dc.subject.other	disinfection	en
dc.subject.other	Lactobacillus fermentum	en
dc.subject.other	Listeria monocytogenes	en
dc.subject.other	monoculture	en
dc.subject.other	nonhuman	en
dc.subject.other	pH	en
dc.subject.other	Pseudomonas putida	en
dc.subject.other	Salmonella enterica	en
dc.subject.other	satureja thymbra	en
dc.subject.other	savory	en
dc.subject.other	Staphylococcus simulans	en
dc.subject.other	Bacteria	en
dc.subject.other	Biofilms	en
dc.subject.other	Disinfectants	en
dc.subject.other	Disinfection	en
dc.subject.other	Equipment Contamination	en
dc.subject.other	Humans	en
dc.subject.other	Hydrochloric Acid	en
dc.subject.other	Hydrogels	en
dc.subject.other	Hydrogen-Ion Concentration	en
dc.subject.other	Industrial Microbiology	en
dc.subject.other	Lactic Acid	en
dc.subject.other	Oils, Volatile	en
dc.subject.other	Plant Preparations	en
dc.subject.other	Satureja	en
dc.subject.other	Stainless Steel	en
dc.subject.other	Bacteria (microorganisms)	en
dc.subject.other	Lactobacillus fermentum	en
dc.subject.other	Listeria monocytogenes	en
dc.subject.other	Pseudomonas putida	en
dc.subject.other	Salmonella enterica	en
dc.subject.other	Satureja thymbra	en
dc.subject.other	Staphylococcus simulans	en
dc.title	Disinfectant test against monoculture and mixed-culture biofilms composed of technological, spoilage and pathogenic bacteria: Bactericidal effect of essential oil and hydrosol of Satureja thymbra and comparison with standard acid-base sanitizers	en
heal.type	journalArticle	en
heal.identifier.primary	10.1111/j.1365-2672.2007.03694.x	en
heal.publicationDate	2008	en
heal.abstract	Aims: To assess the antimicrobial action of three natural-derived products (essential oil, decoction and hydrosol of Satureja thymbra) against biofilms, composed of useful, spoilage and pathogenic bacteria (formed as monoculture or/and mixed-culture), and to compare their efficiency with three standard acid and alkaline chemical disinfectants. Methods and Results: Two acids (hydrochloric and lactic, pH 3), one alkali (sodium hydroxide, pH 11), the essential oil of S. thymbra (1% v/v) and the two by-products of the essential oil purification procedure (the decoction and the hydrosol fraction of essential oil, 100%), were tested against biofilms formed by five bacterial species, either as monospecies, or as mixed-culture of all species. The tested bacterial species were Staphylococcus simulans and Lactobacillus fermentum (useful technological bacteria), Pseudomonas putida (spoilage bacterium), Salmonella enterica and Listeria monocytogenes (pathogenic bacteria). Biofilms were left to be formed on stainless steel coupons for 5 days at 16°C, before the application of disinfection treatments, for 60 and 180 min. The disinfection efficiency was evaluated by detaching the remaining viable biofilm cells and enumerating them by agar plating, as well as by automated conductance measurements (using Rapid Automated Bacterial Impedance Technique). Both these methods revealed that the essential oil and the hydrosol of S. thymbra exhibited a strong antimicrobial action against both monospecies and mixed-culture biofilms. Surprisingly, the efficiency of the other three acid-base disinfectants was not adequate, although a long antimicrobial treatment was applied (180 min). Conclusions: The essential oil of S. thymbra (1%), as well as its hydrosol fraction (100%), presents sufficient bactericidal effect on bacterial biofilms formed on stainless steel. Significance and Impact of the Study: Use of natural antimicrobial agents could provide alternative or supplemented ways for the disinfection of microbial-contaminated industrial surfaces. © 2008 The Authors.	en
heal.journalName	Journal of Applied Microbiology	en
dc.identifier.issue	6	en
dc.identifier.volume	104	en
dc.identifier.doi	10.1111/j.1365-2672.2007.03694.x	en
dc.identifier.spage	1586	en
dc.identifier.epage	1596	en