Cost-effective production of a vaginal protein microbicide to prevent HIV transmission

dc.contributor.advisor	Ramessar, Koreen	en
dc.contributor.author	Rademacher, Thomas	en
dc.contributor.author	Sack, Markus	en
dc.contributor.author	Stadlmann, Johannes	en
dc.contributor.author	Platis, Dimitris	en
dc.contributor.author	Stiegler, Gabriela	en
dc.contributor.author	Labrou, Nikos	en
dc.contributor.author	Altmann, Fritz	en
dc.contributor.author	Ma, Julian	en
dc.contributor.author	Stoger, Eva	en
dc.contributor.author	Capell, Teresa	en
dc.contributor.author	Christou, Paul	en
dc.contributor.author	Πλατής, Δημήτρης	el
dc.contributor.author	Χρήστου, Παύλος	el
dc.contributor.author	Λάμπρου, Νίκος	el
dc.date.accessioned	2014-06-06T06:48:24Z
dc.date.available	2014-06-06T06:48:24Z
dc.date.issued	2008-03-11	en
dc.identifier.issn	00278424	en
dc.identifier.uri	http://dx.doi.org/10.1073/pnas.0708841104	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4129
dc.rights	CC0 1.0 Παγκόσμια
dc.rights.uri	http://creativecommons.org/publicdomain/zero/1.0/
dc.title	Cost-effective production of a vaginal protein microbicide to prevent HIV transmission	en
heal.type	journalArticle	en
heal.keyword	Monoclonal antibody	en
heal.keyword	Staphylococcus protein A	en
heal.keyword	CHO cell	en
heal.keyword	Cricetulus	en
heal.keyword	Human immunodeficiency	en
heal.keyword	Virus infection	en
heal.keyword	Metabolism	en
heal.keyword	Polyacrylamide gel	en
heal.keyword	Electrophoresis	en
heal.keyword	Serodiagnosis	en
heal.keyword	Glycopeptides	en
heal.keyword	HIV Antibodies	en
heal.keyword	HIV Antigens	en
heal.keyword	HIV Infections	en
heal.identifier.primary	10.1073/pnas.0708841104	en
heal.recordProvider	Universitat de Lleida/Departament de Produccio "Vegetal i Cie" ncia Forestal	it
heal.recordProvider	Institute for Molecular Biotechnology, Biology VII, Aachen, Germany	en
heal.recordProvider	University of Natural Resources and Applied Life Sciences, Vienna/Department of Chemistry, Glycobiology Division	en
heal.recordProvider	Γεωπονικό Πανεπιστήμιο Αθηνών/ Τμήμα Γεωπονικής Βιοτεχνολογίας	el
heal.recordProvider	Polymun Scientific Vienna	en
heal.recordProvider	Saint George’s University of London	en
heal.recordProvider	Institucio Catalana de Recerca i Estudis Avancats Barcelona, Spain	it
heal.publicationDate	2008-03-11	en
heal.bibliographicCitation	Ramessar, Koreen. Cost-effective production of a vaginal protein microbicide to prevent HIV transmission, PNAS, vol. 105 (10), pp. 3727-3732, The National Academy of Sciences USA, 2008	en
heal.abstract	A series of small-molecule microbicides has been developed for vaginal delivery to prevent heterosexual HIV transmission, but results from human clinical trials have been disappointing. Proteinbased microbicides, such as HIV-specific monoclonal antibodies, have been considered as an alternative approach. Despite their promising safety profile and efficacy, the major drawback of such molecules is the economy of large-scale production in mammalian cells, the current system of choice. Here, we show that an alternative biomanufacturing platform is now available for one of the most promising anti-HIV antibodies (2G12). Our data show that the HIV-neutralization capability of the antibody is equal to or superior to that of the same antibody produced in CHO cells. We conclude that this protein production system may provide a means to achieve microbicide ingredient manufacture at costs that would allow product introduction and manufacture in the developing world. © 2008 by The National Academy of Sciences of the USA.	en
heal.publisher	The National Academy of Sciences USA	en
heal.journalName	Proceedings of the National Academy of Sciences of the United States of America (PNAS)	en
dc.identifier.doi	10.1073/pnas.0708841104	en