Characterization of spermidine and spermine synthases in Lotus japonicus: Induction and spatial organization of polyamine biosynthesis in nitrogen fixing nodules

dc.contributor.author	Efrose, RC	en
dc.contributor.author	Flemetakis, E	en
dc.contributor.author	Sfichi, L	en
dc.contributor.author	Stedel, C	en
dc.contributor.author	Kouri, ED	en
dc.contributor.author	Udvardi, MK	en
dc.contributor.author	Kotzabasis, K	en
dc.contributor.author	Katinakis, P	en
dc.date.accessioned	2014-06-06T06:48:22Z
dc.date.available	2014-06-06T06:48:22Z
dc.date.issued	2008	en
dc.identifier.issn	00320935	en
dc.identifier.uri	http://dx.doi.org/10.1007/s00425-008-0717-1	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4114
dc.subject	Lotus	en
dc.subject	Polyamines	en
dc.subject	Spermidine synthase	en
dc.subject	Spermine synthase	en
dc.subject	Symbiotic nitrogen fixation	en
dc.subject.other	polyamine	en
dc.subject.other	spermidine synthase	en
dc.subject.other	spermine synthase	en
dc.subject.other	vegetable protein	en
dc.subject.other	amino acid sequence	en
dc.subject.other	article	en
dc.subject.other	enzymology	en
dc.subject.other	genetic complementation	en
dc.subject.other	genetic transcription	en
dc.subject.other	genetics	en
dc.subject.other	in situ hybridization	en
dc.subject.other	Lotus	en
dc.subject.other	metabolism	en
dc.subject.other	molecular genetics	en
dc.subject.other	nodulation	en
dc.subject.other	reverse transcription polymerase chain reaction	en
dc.subject.other	sequence homology	en
dc.subject.other	Amino Acid Sequence	en
dc.subject.other	Genetic Complementation Test	en
dc.subject.other	In Situ Hybridization	en
dc.subject.other	Lotus	en
dc.subject.other	Molecular Sequence Data	en
dc.subject.other	Plant Proteins	en
dc.subject.other	Polyamines	en
dc.subject.other	Reverse Transcriptase Polymerase Chain Reaction	en
dc.subject.other	Root Nodules, Plant	en
dc.subject.other	Sequence Homology, Amino Acid	en
dc.subject.other	Spermidine Synthase	en
dc.subject.other	Spermine Synthase	en
dc.subject.other	Transcription, Genetic	en
dc.subject.other	Lotus corniculatus var. japonicus	en
dc.title	Characterization of spermidine and spermine synthases in Lotus japonicus: Induction and spatial organization of polyamine biosynthesis in nitrogen fixing nodules	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00425-008-0717-1	en
heal.publicationDate	2008	en
heal.abstract	The biosynthesis of the polyamines spermidine (Spd) and spermine (Spm) from putrescine (Put) is catalysed by the consequent action of two aminopropyltransferases, spermidine synthase (SPDS EC: 2.5.1.16) and spermine synthase (SPMS EC: 2.5.1.22). Two cDNA clones coding for SPDS and SPMS homologues in the nitrogen-fixing nodules of the model legume Lotus japonicus were identified. Functionality of the encoded polypeptides was confirmed by their ability to complement spermidine and spermine deficiencies in yeast. The temporal and spatial expression pattern of the respective genes was correlated with the accumulation of total polyamines in symbiotic and non-symbiotic organs. Expression of both genes was maximal at early stages of nodule development, while at later stages the levels of both transcripts declined. Both genes were expressed in nodule inner cortical cells, vascular bundles, and central tissue. In contrast to gene expression, increasing amounts of Put, Spd, and Spm were found to accumulate during nodule development and after maturity. Interestingly, nodulated plants exhibited systemic changes in both LjSPDS and LjSPMS transcript levels and polyamine content in roots, stem and leaves, in comparison to uninoculated plants. These results give new insights into the neglected role of polyamines during nodule development and symbiotic nitrogen fixation (SNF). © 2008 Springer-Verlag.	en
heal.journalName	Planta	en
dc.identifier.issue	1	en
dc.identifier.volume	228	en
dc.identifier.doi	10.1007/s00425-008-0717-1	en
dc.identifier.spage	37	en
dc.identifier.epage	49	en