Biotechnological valorisation of raw glycerol discharged after bio-diesel (fatty acid methyl esters) manufacturing process: Production of 1,3-propanediol, citric acid and single cell oil

dc.contributor.author	Papanikolaou, S	en
dc.contributor.author	Fakas, S	en
dc.contributor.author	Fick, M	en
dc.contributor.author	Chevalot, I	en
dc.contributor.author	Galiotou-Panayotou, M	en
dc.contributor.author	Komaitis, M	en
dc.contributor.author	Marc, I	en
dc.contributor.author	Aggelis, G	en
dc.date.accessioned	2014-06-06T06:48:22Z
dc.date.available	2014-06-06T06:48:22Z
dc.date.issued	2008	en
dc.identifier.issn	09619534	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.biombioe.2007.06.007	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4110
dc.subject	1,3-Propanediol	en
dc.subject	Bio-diesel	en
dc.subject	Citric acid	en
dc.subject	Clostridium butyricum	en
dc.subject	Fatty acid methyl esters	en
dc.subject	Mortierella isabellina	en
dc.subject	Raw glycerol	en
dc.subject	Single-cell oil	en
dc.subject	Yarrowia lipolytica	en
dc.subject.other	Citric acid	en
dc.subject.other	Concentration (process)	en
dc.subject.other	Esters	en
dc.subject.other	Substrates	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	Fatty acid methyl esters	en
dc.subject.other	Mortierella isabellina	en
dc.subject.other	Yarrowia lipolytica	en
dc.subject.other	Glycerol	en
dc.subject.other	Citric acid	en
dc.subject.other	Concentration (process)	en
dc.subject.other	Esters	en
dc.subject.other	Glycerol	en
dc.subject.other	Substrates	en
dc.subject.other	alcohol	en
dc.subject.other	bacterium	en
dc.subject.other	biofuel	en
dc.subject.other	biotechnology	en
dc.subject.other	carbon	en
dc.subject.other	citric acid	en
dc.subject.other	experimental study	en
dc.subject.other	fatty acid	en
dc.subject.other	fungus	en
dc.subject.other	growth rate	en
dc.subject.other	nitrogen	en
dc.subject.other	substrate	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	Mortierella	en
dc.subject.other	Umbelopsis isabellina	en
dc.subject.other	Yarrowia lipolytica	en
dc.title	Biotechnological valorisation of raw glycerol discharged after bio-diesel (fatty acid methyl esters) manufacturing process: Production of 1,3-propanediol, citric acid and single cell oil	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.biombioe.2007.06.007	en
heal.publicationDate	2008	en
heal.abstract	Raw glycerol, byproduct from bio-diesel production process, is used as carbon substrate in several biotechnological applications. Using Clostridium butyricum F2b, 47.1 g L-1 of 1,3-propanediol was produced in batch anaerobic cultures while substrate uptake rate (rS, expressed in g L-1 h-1) increased with increase in glycerol concentration in the medium. In continuous cultures, microbial behaviour was studied in transitory states after addition of 1,3-propanediol in the chemostat vessel. Microbial growth was not affected by the high 1,3-propanediol (which was added in the chemostat vessel) concentration, while butyric and acetic acids concentrations were increased. In a two-stage continuous culture, 43.5 g L-1 of 1,3-propanediol was produced with a total volumetric productivity of 1.33 g L-1 h-1. Yarrowia lipolytica ACA-DC 50109 was grown in nitrogen-limited aerobic cultures on raw glycerol and it exhibited remarkable biomass production even at high glycerol concentration media, while rS decreased with increase in glycerol concentration. Citric acid was produced after nitrogen depletion in the medium, with the highest quantity of 62.5 g L-1, and yield on glycerol consumed was 0.56 g g-1. Fatty acid analysis of total cellular lipids showed that glycerol concentration increase in the growth medium somehow increased the cellular unsaturated fatty acids content of lipids. Mortierella isabellina ATHUM 2935 exhibited satisfactory growth in nitrogen-limited aerobic cultures with raw glycerol used as sole substrate. When high initial glycerol quantities were employed (e.g. 100 g L-1), 4.4 g L-1 of lipid were accumulated corresponding to around 51% (wt/wt) of lipid in dry weight. rS constantly decreased with increase in glycerol concentration in the medium, and in all cases notable glycerol quantities remained unconsumed in the medium. © 2007 Elsevier Ltd. All rights reserved.	en
heal.journalName	Biomass and Bioenergy	en
dc.identifier.issue	1	en
dc.identifier.volume	32	en
dc.identifier.doi	10.1016/j.biombioe.2007.06.007	en
dc.identifier.spage	60	en
dc.identifier.epage	71	en