| dc.contributor.author |
Ikonomopoulos, J |
en |
| dc.contributor.author |
Liandris, E |
en |
| dc.contributor.author |
Gazouli, M |
en |
| dc.date.accessioned |
2014-06-06T06:48:12Z |
|
| dc.date.available |
2014-06-06T06:48:12Z |
|
| dc.date.issued |
2008 |
en |
| dc.identifier.issn |
04389573 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/4014 |
|
| dc.relation.uri |
http://www.scopus.com/inward/record.url?eid=2-s2.0-77952824285&partnerID=40&md5=00309950de35574f142b95731ef7ecc0 |
en |
| dc.subject |
Diagnosis |
en |
| dc.subject |
Mycobacteria |
en |
| dc.subject |
Nanoparticles |
en |
| dc.subject.other |
DNA topoisomerase (ATP hydrolysing) |
en |
| dc.subject.other |
gold nanoparticle |
en |
| dc.subject.other |
oligonucleotide |
en |
| dc.subject.other |
RNA 16S |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
bacterium detection |
en |
| dc.subject.other |
biosensor |
en |
| dc.subject.other |
crystal |
en |
| dc.subject.other |
DNA isolation |
en |
| dc.subject.other |
microorganism detection |
en |
| dc.subject.other |
Mycobacterium |
en |
| dc.subject.other |
oligonucleotide probe |
en |
| dc.subject.other |
photoluminescence |
en |
| dc.subject.other |
semiconductor |
en |
| dc.subject.other |
Corynebacterineae |
en |
| dc.title |
The design and evaluation of gold nanoparticle-oligonucleotide conjugate probes for the detection of Mycobacteria |
en |
| heal.type |
journalArticle |
en |
| heal.publicationDate |
2008 |
en |
| heal.abstract |
Objective: Nanoparticles are semiconductor crystals whose photoluminescence emission wavelength is proportional to the size of the crystal. Their emission spectra are narrow, which allows multi-wavelength labelling of different sizes of crystals, with little overlap. Within this context, nanoparticles have proved to be very promising for the construction of highly sensitive biosensors that can be incorporated in novel diagnostic assays for the rapid, specific, and multifactorial detection of pathogens. However this requires their combination with biomolecules specifically designed for microbial detection and conjugation. With this goal a set of gold nanoparticle-oligonucleotide conjugates was constructed that allow detection of mycobacterial DNA targets without the need for amplification. Methods: The targets selected for the detection of Mycobacteria were the 16s rRNA, 16s-23 s ITS and gyraseB genes that contain regions highly specific to the mycobacterial species. Oligonucleotide probes were designed using the Primer Premier Software and their specificity was assessed by BLAST Search. The final selection involved a Dot Blot Hybridization assay in order to evaluate the functionality and the specificity of the probes that were hybridized with a panel of DNA isolated from various mycobacterial species and other bacteria that can be found in clinical samples. Results: The conjugates allowed specific detection of the targeted Mycobacteria only. The reaction was demonstrated by a change of colour and was confirmed by spectrophotometry. Conclusions: The diagnostic assay presented here comprises a very simple and reliable alternative for the direct detection of mycobacterial pathogens without the need for amplification or special high-cost equipment. The approach described constitutes a technology platform that could also accommodate the detection of several other microbial pathogens. |
en |
| heal.journalName |
Acta Microbiologica Hellenica |
en |
| dc.identifier.issue |
6 |
en |
| dc.identifier.volume |
53 |
en |
| dc.identifier.spage |
352 |
en |
| dc.identifier.epage |
359 |
en |