| dc.contributor.author |
Zhu, Y |
en |
| dc.contributor.author |
Eiteman, MA |
en |
| dc.contributor.author |
Altman, E |
en |
| dc.date.accessioned |
2014-06-06T06:48:08Z |
|
| dc.date.available |
2014-06-06T06:48:08Z |
|
| dc.date.issued |
2008 |
en |
| dc.identifier.issn |
01415492 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1007/s10529-008-9775-5 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/3983 |
|
| dc.subject |
Dissolved oxygen |
en |
| dc.subject |
Lactate |
en |
| dc.subject |
Succinate |
en |
| dc.subject |
Ultrafiltration |
en |
| dc.subject.other |
Biochemical engineering |
en |
| dc.subject.other |
Concentration (process) |
en |
| dc.subject.other |
Dissolution |
en |
| dc.subject.other |
Ethanol |
en |
| dc.subject.other |
Cell recycling |
en |
| dc.subject.other |
Dissolved oxygen |
en |
| dc.subject.other |
Lactate |
en |
| dc.subject.other |
Succinate |
en |
| dc.subject.other |
Ultrafiltration |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
acetic acid derivative |
en |
| dc.subject.other |
alcohol |
en |
| dc.subject.other |
lactic acid derivative |
en |
| dc.subject.other |
oxygen |
en |
| dc.subject.other |
succinic acid |
en |
| dc.subject.other |
anaerobic growth |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
cytology |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
fermentation |
en |
| dc.subject.other |
growth, development and aging |
en |
| dc.subject.other |
metabolism |
en |
| dc.subject.other |
time |
en |
| dc.subject.other |
ultrafiltration |
en |
| dc.subject.other |
Acetates |
en |
| dc.subject.other |
Anaerobiosis |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
Ethanol |
en |
| dc.subject.other |
Fermentation |
en |
| dc.subject.other |
Lactates |
en |
| dc.subject.other |
Oxygen |
en |
| dc.subject.other |
Succinic Acid |
en |
| dc.subject.other |
Time Factors |
en |
| dc.subject.other |
Ultrafiltration |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.title |
Indirect monitoring of acetate exhaustion and cell recycle improve lactate production by non-growing Escherichia coli |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1007/s10529-008-9775-5 |
en |
| heal.publicationDate |
2008 |
en |
| heal.abstract |
A two-phase, lactate fermentation by Escherichia coli ALS974 generates succinate and ethanol anaerobically from acetate. These by-products can be minimized by monitoring acetate concentration indirectly with dissolved O 2 (DO) during the initial aerobic cell-growth phase. Without DO monitoring, 3 g succinate/l and 1 g ethanol/l were generated while, with monitoring, less than 1 g succinate/l and no detectable ethanol were generated with 130 g lactate/l being produced. Furthermore, using a cell-recycle fermentation with ultrafiltration prolonged the anaerobic lactate production phase from 22 to 34 h, thereby achieving a lactate productivity of 4.2 g/l h, nearly 20% greater than the productivity of the fed-batch process. © 2008 Springer Science+Business Media B.V. |
en |
| heal.journalName |
Biotechnology Letters |
en |
| dc.identifier.issue |
11 |
en |
| dc.identifier.volume |
30 |
en |
| dc.identifier.doi |
10.1007/s10529-008-9775-5 |
en |
| dc.identifier.spage |
1943 |
en |
| dc.identifier.epage |
1946 |
en |