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Hydrolysis of Tifton 85 bermudagrass in a pressurized batch hot water reactor

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dc.contributor.author Brandon, SK en
dc.contributor.author Eiteman, MA en
dc.contributor.author Patel, K en
dc.contributor.author Richbourg, MM en
dc.contributor.author Miller, DJ en
dc.contributor.author Anderson, WF en
dc.contributor.author Peterson, JD en
dc.date.accessioned 2014-06-06T06:48:08Z
dc.date.available 2014-06-06T06:48:08Z
dc.date.issued 2008 en
dc.identifier.issn 02682575 en
dc.identifier.uri http://dx.doi.org/10.1002/jctb.1824 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3980
dc.subject Bermudagrass en
dc.subject Ethanol en
dc.subject Lignocellulosic biomass en
dc.subject Pressurized batch hot water en
dc.subject Pretreatment en
dc.subject.other Biomass en
dc.subject.other Ethanol en
dc.subject.other Fermentation en
dc.subject.other Hydrolysis en
dc.subject.other Bermudagrass en
dc.subject.other Lignocellulosic biomass en
dc.subject.other Pressurized batch hot water reactor en
dc.subject.other Batch reactors en
dc.subject.other alcohol en
dc.subject.other cellulase en
dc.subject.other ferulic acid en
dc.subject.other para coumaric acid en
dc.subject.other sugar en
dc.subject.other Batch reactors en
dc.subject.other Biomass en
dc.subject.other Ethanol en
dc.subject.other Fermentation en
dc.subject.other Hydrolysis en
dc.subject.other alcohol production en
dc.subject.other article en
dc.subject.other bacterial strain en
dc.subject.other batch reactor en
dc.subject.other bermudagrass en
dc.subject.other biotechnological production en
dc.subject.other controlled study en
dc.subject.other digestion en
dc.subject.other dry weight en
dc.subject.other Escherichia coli en
dc.subject.other fermentation en
dc.subject.other grass en
dc.subject.other hydrolysis en
dc.subject.other nonhuman en
dc.subject.other pressure en
dc.subject.other pressurized batch hot water reactor en
dc.subject.other reaction time en
dc.subject.other temperature en
dc.subject.other Cynodon (angiosperm) en
dc.subject.other Escherichia coli en
dc.title Hydrolysis of Tifton 85 bermudagrass in a pressurized batch hot water reactor en
heal.type journalArticle en
heal.identifier.primary 10.1002/jctb.1824 en
heal.publicationDate 2008 en
heal.abstract Background: Ethanol production from grass is desirable due to the large amount of biomass it produces. However, a pretreatment is necessary before fermentation to increase ethanol yield. Tifton 85 bermudagrass was treated with a newly designed pressurized batch hot water reactor. Multiple temperatures, pressures, and reaction times were evaluated, and reducing sugars liberated during enzymatic hydrolysis were determined. Results: Pressure had a negligible effect on digestibility of the grass, and a reaction temperature of 230°C for 2 min was the most effective in releasing reducing sugars. Fermentations were conducted with untreated grass and with grass treated for 2 min at 200°C or 230°C to confirm that the increase in reducing sugar concentration resulted in an increased ethanol yield. Following hydrolysis with 2 filter paper units (FPU) of a mixed cellulase enzyme cocktail per gram dry weight of grass, fermentations were performed with engineered Escherichia coli strain LY01. Grass treated at 230°C produced 14.7g L -1 of ethanol, which was significantly higher than 200°C treated grass (11.0 g L -1) and untreated grass (9.0 g L -1). Ferulic and para-coumaric acids were also released during the fermentations. Conclusion: Pressurized batch hot water reactor pretreatment is effective in increasing ethanol yield of grass in fermentations. © 2008 Society of Chemical Industry. en
heal.journalName Journal of Chemical Technology and Biotechnology en
dc.identifier.issue 4 en
dc.identifier.volume 83 en
dc.identifier.doi 10.1002/jctb.1824 en
dc.identifier.spage 505 en
dc.identifier.epage 512 en


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