| dc.contributor.author |
Vasiliou, EG |
en |
| dc.contributor.author |
Makarovska, YM |
en |
| dc.contributor.author |
Pneumatikos, IA |
en |
| dc.contributor.author |
Lolis, NV |
en |
| dc.contributor.author |
Kalogeratos, EA |
en |
| dc.contributor.author |
Papadakis, EK |
en |
| dc.contributor.author |
Georgiou, CA |
en |
| dc.date.accessioned |
2014-06-06T06:48:01Z |
|
| dc.date.available |
2014-06-06T06:48:01Z |
|
| dc.date.issued |
2007 |
en |
| dc.identifier.issn |
01035053 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/3916 |
|
| dc.relation.uri |
http://www.scopus.com/inward/record.url?eid=2-s2.0-36148989978&partnerID=40&md5=09077670947e01253009d97a2b14487e |
en |
| dc.subject |
Chemiluminescence |
en |
| dc.subject |
Exhaled breath condensate |
en |
| dc.subject |
Flow injection |
en |
| dc.subject |
Hydrogen peroxide |
en |
| dc.subject |
Portable condensing equipment |
en |
| dc.title |
Hydrogen peroxide assessment in exhaled breath condensate: Condensing equipment-rapid flow injection chemiluminescence method |
en |
| heal.type |
journalArticle |
en |
| heal.publicationDate |
2007 |
en |
| heal.abstract |
Several biomolecules in Exhaled Breath Condensate (EBC) can be used as breath biomarkers providing useful clinical information concerning inflammatory lung diseases. Hydrogen peroxide has a special place among biomarkers as it can be directly linked to oxidative stress built up in the human body. Methods for hydrogen peroxide in EBC are essential for a vast number of studies spanning from basic studies to disease diagnosis. Methods currently in use are not automated, time-consuming and tedious. Moreover, most condensing equipment in use is not portable. To address these, we developed portable condensing equipment as an extra alternative and a fully automated Flow Injection (FI) - Chemiluminescence method for hydrogen peroxide determination. The proposed condensing equipment provides collection efficiency better than 86% allowing the collection of 2-3 mL sample in 20 min. The method involves mixing a luminol solution stream that is used as sample carrier with a cobalt(II) stream. Reaction of EBC with luminol in the presence of cobalt(II) catalyst produces light proportional to hydrogen peroxide concentration. The automated FI method features a 10 nmol L -1 detection limit and good linearity (r=0.997). Precision and recoveries are better than 6.0% RSD and 64.9%, respectively. Comparison with the, commonly used for EBC, manual fluorimetric method showed no significant difference, P > 0.001, while correlation of the methods is high, r=0.94. The method provides automation in sample handling, sample throughput of 180 samples h -1 and low detection limit. ©2007 Sociedade Brasileira de Química. |
en |
| heal.journalName |
Journal of the Brazilian Chemical Society |
en |
| dc.identifier.issue |
5 |
en |
| dc.identifier.volume |
18 |
en |
| dc.identifier.spage |
1040 |
en |
| dc.identifier.epage |
1047 |
en |