Olivibacter sitiensis gen. nov., sp. nov., isolated from alkaline olive-oil mill wastes in the region of Sitia, Crete

dc.contributor.author	Ntougias, S	en
dc.contributor.author	Fasseas, C	en
dc.contributor.author	Zervakis, GI	en
dc.date.accessioned	2014-06-06T06:47:54Z
dc.date.available	2014-06-06T06:47:54Z
dc.date.issued	2007	en
dc.identifier.issn	14665026	en
dc.identifier.uri	http://dx.doi.org/10.1099/ijs.0.64561-0	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3849
dc.subject.other	bacterial DNA	en
dc.subject.other	cysteine	en
dc.subject.other	cytosine	en
dc.subject.other	fatty acid	en
dc.subject.other	fructose	en
dc.subject.other	galactose	en
dc.subject.other	glucose	en
dc.subject.other	guanine	en
dc.subject.other	histidine	en
dc.subject.other	lactose	en
dc.subject.other	menaquinone	en
dc.subject.other	olive oil	en
dc.subject.other	RNA 16S	en
dc.subject.other	sorbitol	en
dc.subject.other	sucrose	en
dc.subject.other	xylose	en
dc.subject.other	article	en
dc.subject.other	bacterial growth	en
dc.subject.other	bacterial strain	en
dc.subject.other	bacterium colony	en
dc.subject.other	bacterium isolation	en
dc.subject.other	chemotaxonomy	en
dc.subject.other	DNA content	en
dc.subject.other	Gram negative bacterium	en
dc.subject.other	industrial waste	en
dc.subject.other	new genus	en
dc.subject.other	new species	en
dc.subject.other	nonhuman	en
dc.subject.other	nucleotide sequence	en
dc.subject.other	Olivibacter sitiensis	en
dc.subject.other	pH	en
dc.subject.other	phylogeny	en
dc.subject.other	priority journal	en
dc.subject.other	RNA sequence	en
dc.subject.other	sequence homology	en
dc.subject.other	Sphingobacteriaceae	en
dc.subject.other	Sphingobacterium	en
dc.subject.other	Sphingobacterium thalpophilum	en
dc.subject.other	temperature	en
dc.subject.other	Aerobiosis	en
dc.subject.other	Amino Acids	en
dc.subject.other	Bacterial Typing Techniques	en
dc.subject.other	Bacteroidetes	en
dc.subject.other	Base Composition	en
dc.subject.other	Carbohydrate Metabolism	en
dc.subject.other	DNA, Bacterial	en
dc.subject.other	DNA, Ribosomal	en
dc.subject.other	Fatty Acids	en
dc.subject.other	Genes, rRNA	en
dc.subject.other	Greece	en
dc.subject.other	Hydrogen-Ion Concentration	en
dc.subject.other	Industrial Waste	en
dc.subject.other	Molecular Sequence Data	en
dc.subject.other	Movement	en
dc.subject.other	Phylogeny	en
dc.subject.other	Pigments, Biological	en
dc.subject.other	Plant Oils	en
dc.subject.other	Quinones	en
dc.subject.other	RNA, Bacterial	en
dc.subject.other	RNA, Ribosomal, 16S	en
dc.subject.other	Spores, Bacterial	en
dc.subject.other	Temperature	en
dc.subject.other	Bacteria (microorganisms)	en
dc.subject.other	Negibacteria	en
dc.subject.other	Pedobacter	en
dc.subject.other	Sphingobacteriaceae	en
dc.subject.other	Sphingobacterium	en
dc.subject.other	Sphingobacterium thalpophilum	en
dc.title	Olivibacter sitiensis gen. nov., sp. nov., isolated from alkaline olive-oil mill wastes in the region of Sitia, Crete	en
heal.type	journalArticle	en
heal.identifier.primary	10.1099/ijs.0.64561-0	en
heal.publicationDate	2007	en
heal.abstract	A novel, Gram-negative, non-motile, non-sporulating, rod-shaped bacterium isolated from a viscous two-phase olive-oil mill waste ('alpeorujo') is described. The strain, designated AW-6T, is an obligate aerobe, forming irregular, pigmented creamy white colonies. The pH and temperature ranges for growth were pH 5-8 and 5-45 °C, with optimal pH and temperature for growth of pH 6-7 and 28-32 °C, respectively. Strain AW-6T was chemo-organotrophic and utilized mostly D(+)-glucose, protocatechuate and D(+)-xylose, followed by L-cysteine, D(-)-fructose, D(+)-galactose, L-histidine, lactose, sorbitol and sucrose. Menaquinone-7 was detected in the respiratory chain of strain AW-6T. The major fatty acids of strain AW-6T were C16:1ω7c and/or iso-C15:0 2-OH, iso-C15:0, iso-C17:0 3-OH and C16:0. The closest phylogenetic relative of strain AW-6T was clone Blti35 (89.7% 16S rRNA gene sequence similarity), while Sphingobacterium thalpophilum DSM 11723T was the closest recognized relative within the Sphingobacteriaceae (88.2% similarity). Strain AW-6T showed a low level of DNA-DNA relatedness to S. thalpophilum DSM 11723T (33.8-37.0 %). The DNA G + C content of strain AW-6T was 45.6 mol%. Physiological and chemotaxonomic data further confirmed the distinctiveness of strain AW-6T from members of the genera Sphingobacterium and Pedobacter. Thus, strain AW-6T is considered to represent a novel species of a new genus within the family Sphingobacteriaceae, for which the name Olivibacter sitiensis gen. nov., sp. nov. is proposed. The type strain is AW-6T (= DSM 17696T= CECT 7133T). © 2007 IUMS.	en
heal.journalName	International Journal of Systematic and Evolutionary Microbiology	en
dc.identifier.issue	2	en
dc.identifier.volume	57	en
dc.identifier.doi	10.1099/ijs.0.64561-0	en
dc.identifier.spage	398	en
dc.identifier.epage	404	en