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Morpho-histological study on shoot bud regeneration in cotyledon cultures of pepper (Capsicum annuum)

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dc.contributor.author Mezghani, N en
dc.contributor.author Jemmali, A en
dc.contributor.author Elloumi, N en
dc.contributor.author Gargouri-Bouzid, R en
dc.contributor.author Kintzios, S en
dc.date.accessioned 2014-06-06T06:47:53Z
dc.date.available 2014-06-06T06:47:53Z
dc.date.issued 2007 en
dc.identifier.issn 00063088 en
dc.identifier.uri http://dx.doi.org/10.2478/s11756-007-0146-9 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3843
dc.subject Adventitious organogenesis en
dc.subject Cotyledon explant en
dc.subject Histology en
dc.subject Pepper en
dc.subject Protuberances en
dc.subject.other Capsicum annuum en
dc.title Morpho-histological study on shoot bud regeneration in cotyledon cultures of pepper (Capsicum annuum) en
heal.type journalArticle en
heal.identifier.primary 10.2478/s11756-007-0146-9 en
heal.publicationDate 2007 en
heal.abstract Adventitious organogenetic structures were produced in vitro using cotyledon explants excised from 14 day-old pepper seedlings. The best response was observed on MS medium containing 5.7 μM indole-3-acetic acid and 8.8 μM 6-benzylaminopurine. However, when transferred onto elongation medium (MS + 2.8 μM gibberellic acid), these structures frequently developed into leaf-like features rather than into normal shoots. Interestingly, the histological study conducted on the cut end of the cotyledonary petiole revealed a direct induction of numerous teratological protuberances that arise around the cut end of the explant. On the contrary, typical organized bud meristems were rarely observed. Thus, the low number of plants obtained after transfer onto elongation medium seems to be a consequence of this teratological developmental process, frequently associated with fasciated and degenerative meristems, rather than a defect in shoot development from available meristems. © 2007 Institute of Molecular Biology, Slovak Academy of Sciences. en
heal.journalName Biologia - Section Botany en
dc.identifier.issue 6 en
dc.identifier.volume 62 en
dc.identifier.doi 10.2478/s11756-007-0146-9 en
dc.identifier.spage 704 en
dc.identifier.epage 710 en


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