Modeling the effect of storage atmosphere on growth-no growth interface of Listeria monocytogenes as a function of temperature, sodium lactate, sodium diacetate, and NaCl

dc.contributor.author	Skandamis, PN	en
dc.contributor.author	Stopforth, JD	en
dc.contributor.author	Yoon, Y	en
dc.contributor.author	Kendall, PA	en
dc.contributor.author	Sofos, JN	en
dc.date.accessioned	2014-06-06T06:47:53Z
dc.date.available	2014-06-06T06:47:53Z
dc.date.issued	2007	en
dc.identifier.issn	0362028X	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3836
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-35348896682&partnerID=40&md5=8fadd49208cebdfef76c08f7e4241026	en
dc.subject.other	acetic acid	en
dc.subject.other	food preservative	en
dc.subject.other	lactate sodium	en
dc.subject.other	oxygen	en
dc.subject.other	sodium chloride	en
dc.subject.other	article	en
dc.subject.other	bacterial count	en
dc.subject.other	biological model	en
dc.subject.other	dose response	en
dc.subject.other	drug combination	en
dc.subject.other	drug effect	en
dc.subject.other	drug potentiation	en
dc.subject.other	food contamination	en
dc.subject.other	food handling	en
dc.subject.other	food preservation	en
dc.subject.other	growth, development and aging	en
dc.subject.other	Listeria monocytogenes	en
dc.subject.other	metabolism	en
dc.subject.other	methodology	en
dc.subject.other	pH	en
dc.subject.other	risk assessment	en
dc.subject.other	temperature	en
dc.subject.other	Colony Count, Microbial	en
dc.subject.other	Dose-Response Relationship, Drug	en
dc.subject.other	Drug Combinations	en
dc.subject.other	Drug Synergism	en
dc.subject.other	Food Contamination	en
dc.subject.other	Food Handling	en
dc.subject.other	Food Preservation	en
dc.subject.other	Food Preservatives	en
dc.subject.other	Hydrogen-Ion Concentration	en
dc.subject.other	Listeria monocytogenes	en
dc.subject.other	Models, Biological	en
dc.subject.other	Oxygen	en
dc.subject.other	Risk Assessment	en
dc.subject.other	Sodium Acetate	en
dc.subject.other	Sodium Chloride	en
dc.subject.other	Sodium Lactate	en
dc.subject.other	Temperature	en
dc.subject.other	Listeria monocytogenes	en
dc.title	Modeling the effect of storage atmosphere on growth-no growth interface of Listeria monocytogenes as a function of temperature, sodium lactate, sodium diacetate, and NaCl	en
heal.type	journalArticle	en
heal.publicationDate	2007	en
heal.abstract	The effect of aerobic and anaerobic conditions on growth initiation by a 10-strain composite of Listeria monocytogenes (104 CFU/ml) was evaluated in tryptic soy broth with 0.6% yeast extract (TSBYE) as a function of 220 combinations of pH (3.82 to 7.42), sodium lactate (SL) (0 to 10%, vol/vol), and sodium diacetate (SD) (0 to 0.5%, wt/vol) at 10 or 30°C (a slightly abusive and the optimal growth temperature, both above the growth limiting range of 0 to 3°C for L. monocytogenes) in 96-well microplates. In addition, four probability-of-growth models were developed to quantify the effect of 346 aerobic and 346 anaerobic combinations of temperature (4 to 30°C), SL (0 to 6%, vol/vol), and SD (0 to 0.5%, wt/vol) in the presence of NaCl (0.5 or 2.5%, wt/vol) on the growth-no growth responses of the same L. monocytogenes strain composite, with a microplate reader. Growth responses were evaluated turbidimetrically (620 nm) every 5 days for a total of 40 days. Data were modeled with logistic regression to determine the growth-no growth interfaces. The minimum pH values at which growth of L. monocytogenes occurred were higher under anaerobic than under aerobic conditions, and this difference was more evident at 100C or at higher SL and SD concentrations. The MIC of SD decreased with increasing SL levels. Anaerobic storage reduced the levels of SL-SD, allowing the growth of L. monocytogenes compared with aerobic storage, especially at low temperatures. In the presence of 2.5% NaCl, the MICs for SD were lower than those obtained with 0.5% NaCl, especially at 4 and 10°C, or in the presence of 5 to 6% SL. The developed models for anaerobic incubation showed good performance (80% successful predictions; i.e., in 40 of 50 comparisons) with independent data from studies on survival-growth of L. monocytogenes on meat products. The study provides quantitative data on the antimicrobial activity of SL (0 to 10%) and SD (0 to 0.5%), temperature (4 to 30°C), and pH (3.82 to 7.42) and on the probability of growth of L. monocytogenes under anaerobic or aerobic conditions in the presence of 0.5 or 2.5% NaCl, and hence, addresses important needs for risk assessment activities. Copyright ©, International Association for Food Protection.	en
heal.journalName	Journal of Food Protection	en
dc.identifier.issue	10	en
dc.identifier.volume	70	en
dc.identifier.spage	2329	en
dc.identifier.epage	2338	en