Isolation and characterization of ZePES and AtPES, the pescadillo orthologs from Zinnia and Arabidopsis

dc.contributor.author	Zografidis, A	en
dc.contributor.author	Kapolas, G	en
dc.contributor.author	Kitsios, G	en
dc.contributor.author	McCann, M	en
dc.contributor.author	Roberts, K	en
dc.contributor.author	Milioni, D	en
dc.contributor.author	Haralampidis, K	en
dc.date.accessioned	2014-06-06T06:47:52Z
dc.date.available	2014-06-06T06:47:52Z
dc.date.issued	2007	en
dc.identifier.issn	01689452	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.plantsci.2007.06.009	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3825
dc.subject	β-Glucuronidase	en
dc.subject	GFP	en
dc.subject	Nucleolus	en
dc.subject	Pescadillo	en
dc.subject	Yeast complementation	en
dc.subject	Zinnia	en
dc.subject.other	Glucuronidase	en
dc.subject.other	Nucleolus	en
dc.subject.other	Pescadillo	en
dc.subject.other	Yeast complementation	en
dc.subject.other	Zinnia elegans (ZePES)	en
dc.subject.other	Cells	en
dc.subject.other	Enzymes	en
dc.subject.other	Genes	en
dc.subject.other	Growth kinetics	en
dc.subject.other	Yeast	en
dc.subject.other	Plants (botany)	en
dc.subject.other	Animalia	en
dc.subject.other	Arabidopsis	en
dc.subject.other	Arabidopsis thaliana	en
dc.subject.other	Danio rerio	en
dc.subject.other	Zinnia	en
dc.subject.other	Zinnia elegans	en
dc.title	Isolation and characterization of ZePES and AtPES, the pescadillo orthologs from Zinnia and Arabidopsis	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.plantsci.2007.06.009	en
heal.publicationDate	2007	en
heal.abstract	The pescadillo gene was originally identified and characterized through a mutational screen in zebrafish, Danio rerio, affecting embryonic development. Subsequent reports have shown that the yeast and animal homologs are implicated in ribosome biogenesis, cell cycle progression and carcinogenesis, while disruption of its function leads to growth arrest. At present, no orthologs of the pescadillo gene have been characterized in plants. Here we report, for the first time, the isolation and characterization of the pescadillo orthologs from Zinnia elegans (ZePES) and Arabidopsis thaliana (AtPES). ZePES, which is expressed differentially throughout tracheary element differentiation of Zinnia mesophyll cells, is upregulated during G1/S and G2/M transition. Both ZePES and AtPES are able to complement the temperature-sensitive phenotype of yeast degron strain Y40047, impaired in the NOP7/YPH1 gene (pescadillo ortholog), indicating a functional conservation between the yeast and plant gene products. We also show that AtPES-GFP fusion protein is localized in the nucleolus of plant cells. Analysis of transgenic plants reveals that AtPES is expressed during vegetative and reproductive development predominantly in meristematic tissues and actively dividing cells, while overexpression of AtPES does not lead to any phenotypic changes during growth and development. © 2007 Elsevier Ireland Ltd. All rights reserved.	en
heal.journalName	Plant Science	en
dc.identifier.issue	3	en
dc.identifier.volume	173	en
dc.identifier.doi	10.1016/j.plantsci.2007.06.009	en
dc.identifier.spage	358	en
dc.identifier.epage	369	en