Interaction of tRNA with safranal, crocetin, and dimethylcrocetin

dc.contributor.author	Kanakis, CD	en
dc.contributor.author	Tarantilis, PA	en
dc.contributor.author	Tajmir-Riahi, H-A	en
dc.contributor.author	Polissiou, MG	en
dc.date.accessioned	2014-06-06T06:47:51Z
dc.date.available	2014-06-06T06:47:51Z
dc.date.issued	2007	en
dc.identifier.issn	07391102	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3819
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-34250714871&partnerID=40&md5=54bb4561ec97ac894908f0f49d33ee31	en
dc.subject	Binding constant	en
dc.subject	Binding sites	en
dc.subject	Conformation	en
dc.subject	Crocetin	en
dc.subject	Crocus sativus L.	en
dc.subject	Dimethylcrocetin	en
dc.subject	FT-IR	en
dc.subject	Saffron	en
dc.subject	Safranal	en
dc.subject	tRNA	en
dc.subject	UV-Visible spectroscopy	en
dc.subject.other	carotenoid	en
dc.subject.other	crocetin	en
dc.subject.other	Crocus sativus extract	en
dc.subject.other	dimethylcrocetin	en
dc.subject.other	safranal	en
dc.subject.other	transfer RNA	en
dc.subject.other	unclassified drug	en
dc.subject.other	aqueous solution	en
dc.subject.other	article	en
dc.subject.other	binding affinity	en
dc.subject.other	complex formation	en
dc.subject.other	Crocus	en
dc.subject.other	crocus sativus	en
dc.subject.other	drug binding	en
dc.subject.other	infrared spectroscopy	en
dc.subject.other	molecular interaction	en
dc.subject.other	priority journal	en
dc.subject.other	RNA conformation	en
dc.subject.other	ultraviolet spectroscopy	en
dc.subject.other	Binding Sites	en
dc.subject.other	Carotenoids	en
dc.subject.other	Cyclohexenes	en
dc.subject.other	Drug Stability	en
dc.subject.other	Models, Molecular	en
dc.subject.other	RNA, Transfer	en
dc.subject.other	Solutions	en
dc.subject.other	Terpenes	en
dc.subject.other	Crocus sativus	en
dc.title	Interaction of tRNA with safranal, crocetin, and dimethylcrocetin	en
heal.type	journalArticle	en
heal.publicationDate	2007	en
heal.abstract	Saffron is the red dried stigmas of Crocus sativus L. flowers and used both as a spice and as a drug in traditional therapeutic. The biological activity of saffron in modern medicine is in development. Its numerous applications as an anti-oxidant and anti-cancer agent are due to its secondary metabolites and their derivatives (safranal, crocins, crocetin, dimethylcrocetin). The aim of this study was to examine the interaction of transfer RNA with safranal, crocetin, and dimethylcrocetin in aqueous solution at physiological conditions. Constant tRNA concentration (6.25 mM) and various drug/tRNA (phosphate) molar ratios of 1/48 to 1/8 were used. FT-IR and UV-Visible difference spectroscopic methods have been applied to determine the drug binding mode, the binding constants and the effects of drug complexation on the stability and conformation of tRNA duplex. External binding mode was observed for safranal crocetin and dimethylcrocetin, with overall binding constants Ksafranal = 6.8 (± 0.34) × 103 M-1, KCRT = 1.4 (± 0.31) × 104 M-1, and KDMCRT = 3.4 (± 0.30) × 104 M-1. Transfer RNA remains in the A-family structure, upon safranal, crocetin and dimethylcrocetin complexation. ©Adenine Press (2007).	en
heal.journalName	Journal of Biomolecular Structure and Dynamics	en
dc.identifier.issue	6	en
dc.identifier.volume	24	en
dc.identifier.spage	537	en
dc.identifier.epage	545	en