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Halotalea alkalilenta gen. nov., sp. nov., a novel osmotolerant and alkalitolerant bacterium from alkaline olive mill wastes, and emended description of the family Halomonadaceae Franzmann et al. 1989, emend. Dobson and Franzmann 1996

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dc.contributor.author Ntougias, S en
dc.contributor.author Zervakis, GI en
dc.contributor.author Fasseas, C en
dc.date.accessioned 2014-06-06T06:47:47Z
dc.date.available 2014-06-06T06:47:47Z
dc.date.issued 2007 en
dc.identifier.issn 14665026 en
dc.identifier.uri http://dx.doi.org/10.1099/ijs.0.65078-0 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3802
dc.subject.other fatty acid en
dc.subject.other glucose en
dc.subject.other maltose en
dc.subject.other olive oil en
dc.subject.other RNA 16S en
dc.subject.other sodium chloride en
dc.subject.other ubiquinone en
dc.subject.other article en
dc.subject.other bacterial strain en
dc.subject.other bacterium isolation en
dc.subject.other Carnimonas nigrificans en
dc.subject.other concentration response en
dc.subject.other Gram negative bacterium en
dc.subject.other Halomonadaceae en
dc.subject.other Halotalea alkalilenta en
dc.subject.other industrial waste en
dc.subject.other new genus en
dc.subject.other new species en
dc.subject.other nonhuman en
dc.subject.other nucleotide sequence en
dc.subject.other oil industry en
dc.subject.other olive en
dc.subject.other pH en
dc.subject.other phylogeny en
dc.subject.other priority journal en
dc.subject.other Proteobacteria en
dc.subject.other RNA sequence en
dc.subject.other sludge en
dc.subject.other Zymobacter en
dc.subject.other Aerobiosis en
dc.subject.other Bacterial Typing Techniques en
dc.subject.other Base Composition en
dc.subject.other DNA, Bacterial en
dc.subject.other DNA, Ribosomal en
dc.subject.other Fatty Acids en
dc.subject.other Genes, rRNA en
dc.subject.other Glucose en
dc.subject.other Halomonadaceae en
dc.subject.other Hydrogen-Ion Concentration en
dc.subject.other Industrial Waste en
dc.subject.other Models, Molecular en
dc.subject.other Molecular Sequence Data en
dc.subject.other Nucleic Acid Conformation en
dc.subject.other Nucleic Acid Hybridization en
dc.subject.other Olea en
dc.subject.other Organic Chemicals en
dc.subject.other Osmotic Pressure en
dc.subject.other Phylogeny en
dc.subject.other Quinones en
dc.subject.other RNA, Bacterial en
dc.subject.other RNA, Ribosomal, 16S en
dc.subject.other Sequence Analysis, DNA en
dc.subject.other Sequence Homology, Nucleic Acid en
dc.subject.other Sodium Chloride en
dc.subject.other Bacteria (microorganisms) en
dc.subject.other Carnimonas en
dc.subject.other Carnimonas nigrificans en
dc.subject.other Halomonadaceae en
dc.subject.other Halomonas en
dc.subject.other Negibacteria en
dc.subject.other Zymobacter en
dc.subject.other Zymobacter palmae en
dc.title Halotalea alkalilenta gen. nov., sp. nov., a novel osmotolerant and alkalitolerant bacterium from alkaline olive mill wastes, and emended description of the family Halomonadaceae Franzmann et al. 1989, emend. Dobson and Franzmann 1996 en
heal.type journalArticle en
heal.identifier.primary 10.1099/ijs.0.65078-0 en
heal.publicationDate 2007 en
heal.abstract A novel Gram-negative, motile, nonsporulating, rod-shaped bacterium isolated from alkaline sludge-like wastes ('alpeorujo' or 'alperujo') of two-phase olive oil extraction is described. The strain, designated AW-7T, is an obligate aerobe that is halotolerant (tolerating up to 15% w/v NaCl), sugar-tolerant (tolerating up to 45% and 60% W/V (+),-D-glucose and maltose respectively; these are the highest concentrations tolerated by any known members of the Bacteria domain) and alkalitolerant (growing at a broad pH range of 5-11). Strain AW-7T is chemo-organotrophic. Ubiquinone-9 was detected in the respiratory chain of strain AW-7T. The major fatty acids present are C18:1ω7c, C16:0, C19:0 cyclo ω8c, C12:0 3-OH and C16:1ω7C/iso-C15:0 2-OH. Based on 16S rRNA gene sequence analysis, strain AW-7T showed almost equal phylogenetic distances from Zymobacter palmae (95.6 % similarity) and Carnimonas nigrificans (95.4 % similarity). In addition, low DNA-DNA relatedness values were found for strain AW-7T against Carnimonas nigrificans CECT 4437T (22.5-25.4%) and Z. palmae DSM 10491T (11.9-14.4%). The DNA G+C content of strain AW-7T is 64.4 mol%. Physiological and chemotaxonomic data further confirmed the differentiation of strain AW-7T from the genera Zymobacter and Carnimonas. Thus, strain AW-7T represents a novel bacterial genus within the family Halomonadaceae, for which the name Halotalea gen. nov. is proposed. Halotalea alkalilenta sp. nov. (type strain AW-7T = DSM 17697T = CECT 7134T) is proposed as the type species of the genus Halotalea gen. nov. A reassignment of the descriptive 16S rRNA signature characteristics of the family Halomonadaceae permitted the placement of the novel genus Halotalea into the family; in contrast, the genus Halovibrio possessed only 12 out of the 18 signature characteristics proposed, and hence it was excluded from the family Halomonadaceae. © 2007 IUMS. en
heal.journalName International Journal of Systematic and Evolutionary Microbiology en
dc.identifier.issue 9 en
dc.identifier.volume 57 en
dc.identifier.doi 10.1099/ijs.0.65078-0 en
dc.identifier.spage 1975 en
dc.identifier.epage 1983 en


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