DNA interaction with saffron's secondary metabolites safranal, crocetin, and dimethylcrocetin

dc.contributor.author	Kanakis, CD	en
dc.contributor.author	Tarantilis, PA	en
dc.contributor.author	Tajmir-Riahi, H-A	en
dc.contributor.author	Polissiou, MG	en
dc.date.accessioned	2014-06-06T06:47:41Z
dc.date.available	2014-06-06T06:47:41Z
dc.date.issued	2007	en
dc.identifier.issn	10445498	en
dc.identifier.uri	http://dx.doi.org/10.1089/dna.2006.0529	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3751
dc.subject.other	crocetin	en
dc.subject.other	crocin	en
dc.subject.other	Crocus sativus extract	en
dc.subject.other	dimethylcrocetin	en
dc.subject.other	DNA	en
dc.subject.other	safranal	en
dc.subject.other	unclassified drug	en
dc.subject.other	aqueous solution	en
dc.subject.other	article	en
dc.subject.other	binding affinity	en
dc.subject.other	Crocus	en
dc.subject.other	DNA drug complex	en
dc.subject.other	drug binding site	en
dc.subject.other	drug DNA interaction	en
dc.subject.other	drug structure	en
dc.subject.other	infrared spectroscopy	en
dc.subject.other	metabolite	en
dc.subject.other	nonhuman	en
dc.subject.other	pistil stigma	en
dc.subject.other	priority journal	en
dc.subject.other	ultraviolet spectroscopy	en
dc.subject.other	Animals	en
dc.subject.other	Carotenoids	en
dc.subject.other	Cattle	en
dc.subject.other	Crocus	en
dc.subject.other	Cyclohexenes	en
dc.subject.other	DNA	en
dc.subject.other	Flowers	en
dc.subject.other	Nucleic Acid Conformation	en
dc.subject.other	Plant Preparations	en
dc.subject.other	Solutions	en
dc.subject.other	Spectrophotometry, Ultraviolet	en
dc.subject.other	Spectroscopy, Fourier Transform Infrared	en
dc.subject.other	Terpenes	en
dc.subject.other	Water	en
dc.subject.other	Crocus sativus	en
dc.title	DNA interaction with saffron's secondary metabolites safranal, crocetin, and dimethylcrocetin	en
heal.type	journalArticle	en
heal.identifier.primary	10.1089/dna.2006.0529	en
heal.publicationDate	2007	en
heal.abstract	Saffron comes from the dried red stigmas of the Crocus sativus L. flower. Except for its use in cooking and in traditional medicine, it has numerous applications as an antitoxic, antioxidant, and anticancer agent due to its secondary metabolites and their derivatives (safranal, crocins, crocetin, dimethylcrocetin). However, there has been no information on the interactions of these secondary metabolites with individual DNA at molecular level. This study was designed to examine the interaction of safranal, crocetin (CRT), and dimethylcrocetin (DMCRT) with calf-thymus DNA in aqueous solution at physiological conditions, using constant DNA concentration (6.25 mM) and various drug/DNA(phosphate) molar ratios from 1/48 to 1/2. FTIR and UV-visible difference spectroscopic methods are used to determine the drug binding sites, the binding constants, and the effects of carotenoids and safranal complexation on the stability and conformation of DNA duplex. Both intercalative and external binding modes were observed, with overall binding constants K safranal = 1.24 × 10 3 M -1, K CRT = 6.2 × 10 3 M -1 and K DMCRT = 1.85 × 10 5 M -1 A partial B- to A-DNA transition occurs at high carotenoids and safranal concentrations. © Mary Ann Liebert, Inc.	en
heal.journalName	DNA and Cell Biology	en
dc.identifier.issue	1	en
dc.identifier.volume	26	en
dc.identifier.doi	10.1089/dna.2006.0529	en
dc.identifier.spage	63	en
dc.identifier.epage	70	en