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Discrete roles of a microsomal linoleate desaturase gene in olive identified by spatiotemporal transcriptional analysis

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dc.contributor.author Banilas, G en
dc.contributor.author Nikiforiadis, A en
dc.contributor.author Makariti, I en
dc.contributor.author Moressis, A en
dc.contributor.author Hatzopoulos, P en
dc.date.accessioned 2014-06-06T06:47:40Z
dc.date.available 2014-06-06T06:47:40Z
dc.date.issued 2007 en
dc.identifier.issn 0829318X en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/3749
dc.relation.uri http://www.scopus.com/inward/record.url?eid=2-s2.0-34248368863&partnerID=40&md5=1bce25b7283a37397af4356fe875a2a4 en
dc.subject Drupe development en
dc.subject Fatty acids en
dc.subject Flower en
dc.subject Lipids en
dc.subject Olea europaea en
dc.subject Olive oil en
dc.subject.other acyl coenzyme A desaturase en
dc.subject.other complementary DNA en
dc.subject.other plant DNA en
dc.subject.other biological development en
dc.subject.other developmental stage en
dc.subject.other enzyme activity en
dc.subject.other fatty acid en
dc.subject.other fruit production en
dc.subject.other gene expression en
dc.subject.other physiological response en
dc.subject.other plastid en
dc.subject.other amino acid sequence en
dc.subject.other article en
dc.subject.other endoplasmic reticulum en
dc.subject.other enzymology en
dc.subject.other gene en
dc.subject.other gene expression regulation en
dc.subject.other genetic transcription en
dc.subject.other genetics en
dc.subject.other growth, development and aging en
dc.subject.other in situ hybridization en
dc.subject.other microsome en
dc.subject.other molecular cloning en
dc.subject.other molecular genetics en
dc.subject.other nucleotide sequence en
dc.subject.other olive tree en
dc.subject.other phylogeny en
dc.subject.other sequence homology en
dc.subject.other Amino Acid Sequence en
dc.subject.other Base Sequence en
dc.subject.other Cloning, Molecular en
dc.subject.other DNA, Complementary en
dc.subject.other DNA, Plant en
dc.subject.other Endoplasmic Reticulum en
dc.subject.other Fatty Acid Desaturases en
dc.subject.other Gene Expression Regulation, Developmental en
dc.subject.other Gene Expression Regulation, Enzymologic en
dc.subject.other Gene Expression Regulation, Plant en
dc.subject.other Genes, Plant en
dc.subject.other In Situ Hybridization en
dc.subject.other Microsomes en
dc.subject.other Molecular Sequence Data en
dc.subject.other Olea en
dc.subject.other Phylogeny en
dc.subject.other Sequence Homology, Amino Acid en
dc.subject.other Transcription, Genetic en
dc.subject.other Arabidopsis en
dc.subject.other Lycopersicon esculentum en
dc.subject.other Olea europaea en
dc.title Discrete roles of a microsomal linoleate desaturase gene in olive identified by spatiotemporal transcriptional analysis en
heal.type journalArticle en
heal.publicationDate 2007 en
heal.abstract The relative abundance of α-linolenic (α-LeA) compared with linoleic acid is associated with the developmental stage and the plant species and is proposed to have important physiological effects on both vegetative and reproductive plant development. The enzymes responsible for catalyzing the conversion of linoleic acid to α-LeA, the ω-3 fatty acid desaturases (FADs), are localized in the plastid or the endoplasmic reticulum (ER). Here we present the isolation of an ER-type ω-3 FAD gene (OeFAD3) from olive (Olea europaea L.). Expression patterns of OeFAD3 in different seed tissues and mesocarps during olive fruit development showed that its contribution to olive oil biosynthesis and modification is minimal. Regulation of OeFAD3 differed from that of its plastidial counterpart, being preferentially expressed in proliferating tissues, in concert with the active membrane biogenesis required for cell division. Trienoic acid-deficient Arabidopsis mutants are male sterile, because a-LeA-derived jasmonic acid (JA) is required for pollen development. However, the upregulation of OeFAD3 in different pistil tissues, particularly in vascular bundles and ovaries, rather than in anthers, implies a critical role of α-LeA in female gametophyte development in olive, corroborating results from JA-defective tomato mutants that are female sterile but not male sterile. © 2007 Heron Publishing. en
heal.journalName Tree Physiology en
dc.identifier.issue 4 en
dc.identifier.volume 27 en
dc.identifier.spage 481 en
dc.identifier.epage 490 en


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