Cloning and characterization of an alternative transcript of ovine glucose 6-phosphate dehydrogenase gene: Comparative approach between ruminant and non-ruminant species

dc.contributor.author	Laliotis, GP	en
dc.contributor.author	Argyrokastritis, A	en
dc.contributor.author	Bizelis, I	en
dc.contributor.author	Rogdakis, E	en
dc.date.accessioned	2014-06-06T06:47:38Z
dc.date.available	2014-06-06T06:47:38Z
dc.date.issued	2007	en
dc.identifier.issn	03781119	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.gene.2006.10.016	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3728
dc.subject	Adipose tissue	en
dc.subject	cDNA	en
dc.subject	G6PD	en
dc.subject	Protein model	en
dc.subject	Sheep	en
dc.subject.other	complementary DNA	en
dc.subject.other	glucose 6 phosphate dehydrogenase	en
dc.subject.other	og6pda protein	en
dc.subject.other	og6pdb protein	en
dc.subject.other	polypeptide	en
dc.subject.other	protein	en
dc.subject.other	unclassified drug	en
dc.subject.other	amino acid sequence	en
dc.subject.other	article	en
dc.subject.other	binding site	en
dc.subject.other	bovids	en
dc.subject.other	catalysis	en
dc.subject.other	frameshift mutation	en
dc.subject.other	gene expression	en
dc.subject.other	gene structure	en
dc.subject.other	genetic trait	en
dc.subject.other	genetic transcription	en
dc.subject.other	genetic variability	en
dc.subject.other	lipogenesis	en
dc.subject.other	molecular cloning	en
dc.subject.other	nucleotide sequence	en
dc.subject.other	polymerase chain reaction	en
dc.subject.other	priority journal	en
dc.subject.other	protein analysis	en
dc.subject.other	protein isolation	en
dc.subject.other	reverse transcription	en
dc.subject.other	Southern blotting	en
dc.subject.other	stimulus	en
dc.subject.other	Alternative Splicing	en
dc.subject.other	Amino Acid Sequence	en
dc.subject.other	Animals	en
dc.subject.other	Base Sequence	en
dc.subject.other	Blotting, Southern	en
dc.subject.other	Catalytic Domain	en
dc.subject.other	Cloning, Molecular	en
dc.subject.other	DNA	en
dc.subject.other	DNA, Complementary	en
dc.subject.other	Female	en
dc.subject.other	Gene Expression Profiling	en
dc.subject.other	Glucosephosphate Dehydrogenase	en
dc.subject.other	Isoenzymes	en
dc.subject.other	Male	en
dc.subject.other	Models, Molecular	en
dc.subject.other	Molecular Sequence Data	en
dc.subject.other	Protein Structure, Tertiary	en
dc.subject.other	Reverse Transcriptase Polymerase Chain Reaction	en
dc.subject.other	Ruminants	en
dc.subject.other	Sequence Analysis, DNA	en
dc.subject.other	Sequence Homology, Amino Acid	en
dc.subject.other	Sheep	en
dc.subject.other	Species Specificity	en
dc.subject.other	Ovis	en
dc.subject.other	Ovis aries	en
dc.title	Cloning and characterization of an alternative transcript of ovine glucose 6-phosphate dehydrogenase gene: Comparative approach between ruminant and non-ruminant species	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.gene.2006.10.016	en
heal.publicationDate	2007	en
heal.abstract	Glucose 6-phosphate dehydrogenase (G6PD) plays an important role in ruminant's lipogenesis, as it provides necessary compounds of NADPH for the synthesis of fatty acids catalyzing the first committed reaction in the pentose phosphate pathway. In this work the full length ovine glucose 6-phosphate dehydrogenase cDNA was isolated using a polymerase chain reaction based strategy. Two isoforms (OG6PDA and OG6PDB) were detected encoding a protein of 515 and 524 amino acids, respectively. Both deduced amino acid sequences reveal a well conserved protein containing all the important residues for its catalytic role. The extra nine amino acids encoded by OG6PDB cause a frameshift in the polypeptide chain resulting in changes around the area of the potential substrate binding site. A three-dimensional model of ovine G6PD protein shows that this frameshift cause structural changes in the catalytic binding ""pocket"" of the molecule. Southern blot and RT analysis revealed that ovine G6PD appears as a single copy gene while it is expressed, with slight variability, in all tissues analyzed. Moreover, expression analysis of the ovine G6PD isoforms showed that OG6PDB is expressed only in tissues where lipogenesis is high in ruminants. Thus, we hypothesize that in ruminants G6PD may be regulated by the ratio of the two transcripts, according to the existence stimulus. © 2006 Elsevier B.V. All rights reserved.	en
heal.journalName	Gene	en
dc.identifier.issue	1-2	en
dc.identifier.volume	388	en
dc.identifier.doi	10.1016/j.gene.2006.10.016	en
dc.identifier.spage	93	en
dc.identifier.epage	101	en