| dc.contributor.author |
Papadimitriou, K |
en |
| dc.contributor.author |
Pratsinis, H |
en |
| dc.contributor.author |
Nebe-Von-Caron, G |
en |
| dc.contributor.author |
Kletsas, D |
en |
| dc.contributor.author |
Tsakalidou, E |
en |
| dc.date.accessioned |
2014-06-06T06:47:35Z |
|
| dc.date.available |
2014-06-06T06:47:35Z |
|
| dc.date.issued |
2007 |
en |
| dc.identifier.issn |
00992240 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1128/AEM.01244-06 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/3696 |
|
| dc.subject.other |
Bacteria |
en |
| dc.subject.other |
Cells |
en |
| dc.subject.other |
Cloning |
en |
| dc.subject.other |
Fluorescence |
en |
| dc.subject.other |
Iodine compounds |
en |
| dc.subject.other |
pH effects |
en |
| dc.subject.other |
Probes |
en |
| dc.subject.other |
Sorting |
en |
| dc.subject.other |
Carboxyfluorescein |
en |
| dc.subject.other |
Clonal populations |
en |
| dc.subject.other |
Cytometric viability |
en |
| dc.subject.other |
Ratiometric analysis |
en |
| dc.subject.other |
Bioassay |
en |
| dc.subject.other |
adenosine triphosphatase |
en |
| dc.subject.other |
carboxyfluorescein |
en |
| dc.subject.other |
glucose |
en |
| dc.subject.other |
peptidoglycan |
en |
| dc.subject.other |
phosphoenolpyruvate |
en |
| dc.subject.other |
phosphotransferase |
en |
| dc.subject.other |
propidium iodide |
en |
| dc.subject.other |
vancomycin |
en |
| dc.subject.other |
acid |
en |
| dc.subject.other |
assay |
en |
| dc.subject.other |
bacterium |
en |
| dc.subject.other |
enzyme activity |
en |
| dc.subject.other |
flow cytometry |
en |
| dc.subject.other |
measurement method |
en |
| dc.subject.other |
microbial activity |
en |
| dc.subject.other |
tolerance |
en |
| dc.subject.other |
viability |
en |
| dc.subject.other |
acid tolerance |
en |
| dc.subject.other |
acidity |
en |
| dc.subject.other |
animal cell |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
bacterial cell |
en |
| dc.subject.other |
bacterial growth |
en |
| dc.subject.other |
cell clone |
en |
| dc.subject.other |
cell heterogeneity |
en |
| dc.subject.other |
cell selection |
en |
| dc.subject.other |
cell viability |
en |
| dc.subject.other |
cellular stress response |
en |
| dc.subject.other |
chemical composition |
en |
| dc.subject.other |
culture medium |
en |
| dc.subject.other |
enzyme inhibition |
en |
| dc.subject.other |
flow cytometry |
en |
| dc.subject.other |
fluorescence |
en |
| dc.subject.other |
gene induction |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
pH |
en |
| dc.subject.other |
phenotype |
en |
| dc.subject.other |
protein synthesis |
en |
| dc.subject.other |
Streptococcus |
en |
| dc.subject.other |
Streptococcus macedonicus |
en |
| dc.subject.other |
Adaptation, Physiological |
en |
| dc.subject.other |
Cell Wall |
en |
| dc.subject.other |
Flow Cytometry |
en |
| dc.subject.other |
Fluoresceins |
en |
| dc.subject.other |
Fluorescent Dyes |
en |
| dc.subject.other |
Hydrogen-Ion Concentration |
en |
| dc.subject.other |
Phenotype |
en |
| dc.subject.other |
Propidium |
en |
| dc.subject.other |
Streptococcus |
en |
| dc.subject.other |
Streptococcus macedonicus |
en |
| dc.title |
Acid tolerance of Streptococcus macedonicus as assessed by flow cytometry and single-cell sorting |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1128/AEM.01244-06 |
en |
| heal.publicationDate |
2007 |
en |
| heal.abstract |
An in situ flow cytometric viability assay employing carboxyfluorescein diacetate and propidium iodide was used to identify Streptococcus macedonicus acid tolerance phenotypes. The logarithmic-phase acid tolerance response (L-ATR) was evident when cells were (i) left to autoacidify unbuffered medium, (ii) transiently exposed to nonlethal acidic pH, or (iii) systematically grown under suboptimal acidic conditions (acid habituation). Stationary-phase ATR was also detected; this phenotype was gradually degenerated while cells resided at this phase. Single-cell analysis of S. macedonicus during induction of L-ATR revealed heterogeneity in both the ability and the rate of tolerance acquisition within clonal populations. L-ATR was found to be partially dependent on de novo protein synthesis and compositional changes of the cell envelope. Interestingly, acid-habituated cells were interlaced in lengthier chains and exhibited an irregular pattern of active peptidoglycan biosynthesis sites when probed with BODIPY FL vancomycin. L-ATR caused cells to retain their membrane potential after lethal challenge, as judged by ratiometric analysis with oxonol [DiBAC4(3)]. Furthermore, F-ATPase was important during the induction of L-ATR, but in the case of a fully launched response, inhibition of F-ATPase affected acid resistance only partially. Activities of both F-ATPase and the glucose-specific phosphoenolpyruvate-dependent phosphotransferase system were increased after L-ATR induction, distinguishing S. macedonicus from oral streptococci. Finally, the in situ viability assessment was compared to medium-based recovery after single-cell sorting, revealing that the culturability of subpopulations with identical fluorescence characteristics is dependent on the treatments imposed to the cells prior to acid challenge. Copyright © 2007, American Society for Microbiology. All Rights Reserved. |
en |
| heal.journalName |
Applied and Environmental Microbiology |
en |
| dc.identifier.issue |
2 |
en |
| dc.identifier.volume |
73 |
en |
| dc.identifier.doi |
10.1128/AEM.01244-06 |
en |
| dc.identifier.spage |
465 |
en |
| dc.identifier.epage |
476 |
en |