A standard addition method to assay the concentration of biologically interesting polyphenols in grape berries by reversed-phase HPLC

dc.contributor.advisor	Haroutounian, Serkos A.	en
dc.contributor.advisor	Χαρουτουνιάν, Σέρκος Α.	el
dc.contributor.author	Sakkiadi, Aphrodite - Victoria	en
dc.contributor.author	Georgiou, Constantinos A.	en
dc.contributor.author	Γεωργίου, Κωνσταντίνος Α.	el
dc.contributor.author	Σακκιάδη, Αφροδίτη - Βικτωρία	el
dc.date.accessioned	2014-06-06T06:47:35Z
dc.date.available	2014-06-06T06:47:35Z
dc.date.issued	2007-09-27	en
dc.identifier.issn	14203049	en
dc.identifier.uri	http://dx.doi.org/10.3390/12092259	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/3695
dc.rights	CC0 1.0 Παγκόσμια
dc.rights.uri	http://creativecommons.org/publicdomain/zero/1.0/
dc.title	A standard addition method to assay the concentration of biologically interesting polyphenols in grape berries by reversed-phase HPLC	en
heal.type	journalArticle	en
heal.keyword	Flavonoids	en
heal.keyword	Grape berries	en
heal.keyword	Polyphenols	en
heal.keyword	Vitis vinifera	en
heal.keyword	Glycoside	en
heal.keyword	Hydrolysis	en
heal.keyword	Metabolism	en
heal.keyword	Antioxidants	en
heal.keyword	Calibration	en
heal.keyword	Phenols	en
heal.identifier.primary	10.3390/12092259	en
heal.recordProvider	Γεωπονικό Πανεπιστήμιο Αθηνών/Γενικό Τμήμα	el
heal.publicationDate	2007-09-27	en
heal.bibliographicCitation	Haroutounian, Serkos A. A standard addition method to assay the concentration of biologically interesting polyphenols in grape berries by reversed-phase HPLC. Molecules, vol. 12 (9), pp. 2259-2269, MDPI 2007	en
heal.abstract	A reversed-phase HPLC method which allows the simultaneous assay of (+)-catechin, (-)-epicatechin, trans-resveratrol, quercetin and quercetin glycosides in grape berries is described. Kromasil 100 served as stationary phase and a gradient of acetic acid, water and methanol was used. The analytical run requires 42 min for complete sample elution. Satisfactory peak resolution was achieved following a novel extraction process and direct injection of a 20 μL sample. The method was used for the analyses of eighteen samples. Linearities were in the range of 0.98 to 0.999 regression coefficient, for all phenolics, while detection limits ranged from 30 μg mL-1 for trans-resveratrol to 1.5 mg mL-1 for (+)-catechin. Recoveries ranged from 95.1 to 98.7% while the method provided good precision, with standard deviations between 3.5 and 6.1%, n=5.	en
heal.publisher	Molecular Diversity Preservation International (MDPI)	en
heal.journalName	Molecules	en
dc.identifier.doi	10.3390/12092259	en